Determination of the parameters of acute toxicity of «Brovermectin-granulate» on earlings carp


  • Yu. V. Loboiko Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv, Ukraine
  • M. M. Danko Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv, Ukraine
  • O. V. Krushelnytska Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv, Ukraine
  • S. I. Kravets Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv, Ukraine
Keywords: carp, macrocyclic lactones, brovermectin-granulate, acute toxicity

Abstract

The paper presents the results of research to determine the parameters of acute toxicity of «Brovermectin-granulate». The material for the study of acute toxicity were of carp earlings. In experiment used drug «Brovermectin-granulate» (1 g of the drug contains: active actiion substance ivermectin – 3.5 mg, tocopherol acetate – 20 mg). The introduction of the drug  was carried out orally using a probe in anterior intestine of fish. The drug was administered to fish in the form of a homogeneous suspension produced 1 % starch solution individually, in doses of 1000, 4000, 8000, 12000, 16000 and 20000 mg/kg of body weight. For settings acute toxicity determined the general condition and death of fish; for a dose of the drug (DL50) calculated by methods G. Kerber, H. Pershyn, least squares analysis of probit mortality curves for V.B. Prozorovskyj, means three points for B.M. Shtabskyj. At this stage of research it was found lethal (DL100) and maximum tolerated (DL0) dose of «Brovermectin-granulate» for earlings carp. As a result of studies found that 100% death of fish following the dose of 20000 mg/kg (DL100), and for the drug at a dose of 4000 mg/kg (DL0) set to survival of 100% fish. Established that the value of average lethal dose of «Brovermectin-granulate» for fish regardless of the method of calculation mainly coincided and were 10932,8–11200 mg/kg for earlings carp. Thus, the drug «Brovermectin-granulate» for oral administration to fish belongs to grade 4 toxicity – low toxic substances.

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Published
2017-03-03
How to Cite
Loboiko, Y., Danko, M., Krushelnytska, O., & Kravets, S. (2017). Determination of the parameters of acute toxicity of «Brovermectin-granulate» on earlings carp. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Agricultural Sciences, 19(74), 20-23. https://doi.org/10.15421/nvlvet7405