Progesterone receptor (PRG) gene polymorphism and association with litter size in the california rabbit breed
Modern approaches to genetic improvement of species based on genotype-depth evaluation of farm animals and genetic diversity of populations using marker technology. Determination of genotype of animals was carried out using PCR-RFLP. A 558-bp sequence of the rabbit progesterone receptor promoter region (PGR) gene was obtained for 60 animals of the California breed from High Litter Size (HLS) and Low Litter Size (LLS) groups. Found that in individuals with multiple groups rabbit dominated by individuals with heterozygous genotype (0.700), while in animals with low levels litter size – individuals with genotype AA. In addition, the rabbit and the second group were characterized by very high actual heterozygosity (Ho = 0,700), which significantly exceeds the expected heterozygosity (No = 0.455). Among the animals that had low bahatoplidnosti differences between actual and expected assessments heterozygosity less significant (0.400 and 0.320, respectively). Thus, in both groups, there is an excess of heterozygosity, accompanied by negative assessments fixation index. PCR-RFLP for PGR gene (using the Eco31I restriction endonuclease) revealed two genotypes of AA and GA, and the genotype AA yielding a single 558 bp band, and the heterozygote genotype GA yielding all the three bands of 558, 416 and 142 bp. The 2464G > A SNP for PGR gene was not fixed in the HLS and LLS groups, the allele G frequency being 0.350 in the HLS and 0.200 in the LLS group. Whatever belong to a group marked predominance on the number of rabbits for a rabbit genotype GA on individuals who had genotype AA. More significant, the difference was for the rabbit of the I-th group (9.1 and 7.0 rabbits, respectively). The difference in litter size between the AA and GA genotypes was found for rabbits from as the HLS, as LLS group.
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