Determination of the aminotransferase activity in tissues of infected with ectoparasites yearling carp
The paper presents data on changes in the activity of amіnotransferase (aspartate aminotransferase – AST, alanine aminotransferase – ALT) in various organs and tissues of carp at different intensity of infestation with ectoparasites. The material for studying the activity of aminotransferases was one-year-old carp, spontaneously invasive with ectoparasites. For this purpose, twelve groups of fish were formed for 6 individuals in each, with a body weight of 38.0 ± 4.8 g. In four groups of fish (control and three experimental) for the defeat by ectoparasites L. cyprinacea, D. vastator and for mixed infestation. Our studies have shown that the activity of aminotransferases in hepatopancreas and skeletal muscles of carp significantly changes in the lesion of fish by lereneae. In particular, the activity of ALT in hepatopancreas was significantly higher compared to the control group. The tendency to increase was observed in tissues of skeletal muscles. In the study of AST activity, its growth in hepatopancreas and skeletal muscle was determined. For the defeat of fish by dactylosurus, the activity of aminotransferases in carp gills varies considerably. In particular, ALT activity in gills of fish of groups 3 and 4 was significantly higher compared to the control group. At the same time, the activity of ALT in the tissues of the hepatopancreas of the 4th experimental group was likely to increase. The tendency to increase is established in tissues of hepatopancreas and gills in the study of activity of AST. As a result of fish invasion by dactylosurus, the activity of AST in hepatopancreas increased by 1.2 times. At the same time, the growth of the AST index in the gills of the first year of carp in the 3rd and 4th groups was established. In the study of the activity of aminotransferases for mixed infestation, it was found that the activity of ALT and AST in tissues of hepatopancreas, skeletal muscles and gills of groups 2, 3 and 4 was significantly higher compared to the control group.
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