Association of allele variants of IGF-I, GH and PIT-1 genes with production traits of Birkivska Barvista chicken breed


  • R. O. Kulibaba Institute of animal science NAAS
Keywords: chicken, polymorphism, locus, allele, gene, genotype, molecular genetic markers, population, line, productivity

Abstract

The article considers the questions about association between allelic variants of insulin-like growth factor I (IGF-I), growth hormone (GH) and pituitary transcription factor-1 (PIT-1) genes with the egg productivity of the Borkovskaya Barvistaya chicken breed. Using classical PCR and PCR-RFLP methods, the PstI-polymorphism in the 5'UTR fragment of the IGF-I gene, HinfI-polymorphism in the promoter fragment of IGF-I gene, AluI-polymorphism in the fourth intron of the GH gene and indel polymorphism in the second intron of the PIT-1 gene were determined. As a result of the studies the significant differences in egg weight values on 30 weeks of life for HinfI-polymorphism in the promoter fragment of the IGF-I gene were revealed. For individuals with the C/C genotype was specific the large values of this index compared to A/A (51,7 ± 0,61 vs. 47.9 ± 0,92 respectively). For PstI-polymorphism in the 5'UTR of the IGF-I gene was shown the prevalence in the number of eggs for 12 weeks of productivity for individuals with the C1C1 genotype compared to C2C2 (65,2 ± 3,77 vs. 60,9 ± 1,33), but in this case the differences are not significantly, what can be caused by a small number of individuals with C1C1 genotype (12). There were no significant differences for other loci.

References

Wolc, A. (2014). Understanding genomic selection in poultry breeding. World's Poultry Sci. J. 70, 309–314.

Fulton, J.E. (2008). Molecular genetics in a modern poul-try breeding organization. World’s Poultry Sci. J. 64, 171–176.

Khlestkina, E.K. (2014). Molecular markers in genetic studies and breeding. Russ J Genetics. 4(3), 236–244.

Saxena, V.K., Sachdev, A.K., Gopal, R., Pramod, A.B. (2009). Roles of important candidate genes on broiler meat quality. World's Poultry Sci. J. 65, 37–50.

Li, H., Zhu, W., Chen, K., Wu, X., Tang, Q., Gao, Y. (2008). Associations between GHR and IGF-1 gene polymorphisms, and reproductive traits in Wenchang chickens. Turk. J. Vet. Anim. Sci. 32(4), 281–285.

Shahnaz, S., Shadma, F., Rank, D.N., Khanna, K., Joshi, C.G. (2008). Growth hormone gene polymorphism and its correlation with different traits in Bantam and Leghorn chicken. Indian journal of poultry science. 43(2), 123–127.

Enayati, B., Rahimi-Mianji, G. (2009). Genomic growth hormone, growth hormone receptor and transforming growth factor β-3 gene polymorphism in breeder hens of Mazandaran native fowls. African journal of bio-technology. 8(14), 3154–3159.

Nie, Q., Lei, M., Ouyang, J., Zenq, H., Yang, G., Zhang, X. (2005). Identification and characterization of single nucleotide polymorphisms in 12 chicken growth-correlated genes by denaturing high performance liq-uid chromatography. Genet. Sel. Evol. 37(3), 339–360.

Liu, H.C., Kung, H.J., Fulton, J.E., Morgan, R.W., Cheng, H.H. (2001). Growth hormone interacts with the Ma-rek's disease virus SORF2 protein and is associated with disease resistance in chicken. PNAS USA. 98(16), 9203–9208.

Bhattacharya, T.K., Chatterjee, R.N., Priyanka, M. (2012). Polymorphisms of Pit-1 gene and its associa-tion with growth traits in chicken. Poultry Science. 91, 1057–1064.

Nagaraja, S.C., Aggrey, S.E., Yao, J., Zadworny, D., Fair-full, R.W., Kuhnlein, U. (2000). Trait association of a genetic marker near the IGF-I gene in egg-laying chickens. J. Hered. 91, 150–156.

Li, H., Zhu, W., Chen, K., Song, W., Shu, J., Han, W. (2010). Effects of the polymorphisms of GHR gene and IGF-1 gene on egg quality in Wenchang chicken. Research Journal of Poultry Sciences. 3(2), 19–22.

Zhou, H., Mitchell, A.D., McMurtry, J.P., Ashwell, C.M., Lamont, S.J. (2005). Insulin-Like Growth Factor-I Gene Polymorphism Associations with Growth, Body Composition, Skeleton Integrity, and Metabolic Traits in Chickens. Poultry Science. 84, 212–219.

Kulibaba, R.A., Liashenko, Y.V., Yurko, P.S. (2017). Novel AluI-polymorphism in the fourth intron of chicken growth hormone gene. Cytol. Genet. 51(1), 54–59.

Nie, Q., Fang, M., Xie, L., Zhou, M, Liang, Z., Luo, Z., Wang, J., Bi, W., Liang, C., Zhang, W., Zhang, X. (2008). The PIT1 gene polymorphisms were associat-ed with chicken growth traits. BMC Genet. 9, 20–24.

Abstract views: 42
PDF Downloads: 62
Published
2017-10-30
How to Cite
Kulibaba, R. (2017). Association of allele variants of IGF-I, GH and PIT-1 genes with production traits of Birkivska Barvista chicken breed. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Agricultural Sciences, 19(79), 53–57. https://doi.org/10.15421/nvlvet7911