Indicators of functional and antioxidant liver status of rats under oxidative stress conditions and on the action of the liposomal drug “Butaselmevit”
The purpose of the work was to study the parameters of the functional and antioxidant state of the liver in rats under conditions of oxidative stress and at the actions of the liposomal drug “Butaselmevit”. The research was carried out on young white laboratory male rats of the Vistar line with a body weight of 180-200 g, which were kept in the standard conditions of the institute vivarium of the State Scientific Research Control Institute of Veterinary Preparations and Feed Additives. Animals were divided into three groups of 20 animals in each: 1st group (K) intact animals; 2nd group (D1) – rats, affected with tetrachloromethane; group 3 (D2) – rats, affected with tetrachloromethane and treated with Botselmevit liposomal preparation. Toxic lesion of rats was caused by intramuscular administration of 50% tetrachloromethane at a dose of 0.25 ml per 100 g of animal body weight for the first and third day of the study. Animals of the experimental group D2 on the first and third day of the study, an hour after the introduction of tetrachloroethane, additionally administered a liposomal preparation at a dose of 2 ml per 1 kg of body weight of the animal. The composition of this preparation includes the following substances: butafosfan, selenium, methionine, thistle injection and vitamins A, E and D3. The development of oxidative stress in rats caused by intramuscular administration of tetrachloromethane was accompanied by suppression of protein synthesis of the liver, as evidenced by the low level of total protein and the decrease in the albumin-globulin factor. Low levels of albumin and high levels of globulins in the blood of diseased rats indicate an albumin-globulin disproportion. The indicators of liver function were quite high, namely: the level of creatinine increased by 46%, urea by 74% and total bilirubin by 34%. When applying the liposomal drug "Butaselmevit" to rats, under conditions of oxidative stress during research, the normalization of biochemical parameters occurs in the blood. It has been proven that modeling of the stress response in rats of the experimental group leads to a probable decrease in the level of reduced glutathione and the activity of glutathione peroxidase in the second and fifth days of the experiment. When applying the liposomal drug Butaselmevit to rats, under conditions of oxidative stress during the studies, activation of the glutathione linkage of the antioxidant system occurs in the blood, indicating an increase in the level of reduced glutathione and the activity of glutathione peroxidase in the blood of animal data. At the 14th day of the research, the level of reduced glutathione and the activity of glutathione peroxidase in blood of the experimental group D2 were the highest. The results of the research indicate the antioxidant properties of the new liposomal drug “Butaselmevit”.
Gutiy, B.V. (2012). Vplyv hlorydu kadmiyu na intensivnist protsesiv perekisnogo okysnennya lipidiv ta stan sistemy antyoksydantnogo zahystu organizmu shchuriv. Visnyk Sumskogo natsionalnogo agrarnogo universitetu, 7(31), 31–34 (in Ukrainian).
Gutyj, B.V. (2016). Osobennosti funkcionirovanija sistemy antioksidantnoj zashhity organizma krys pri kadmievom toksikoze. Nauchno-prakticheskij zhurnal. Uchenye Zapiski, 52(2), 24–28 (in Russian).
Calabrese, E., Leonard, D., & Zhao, X. (1996). Role of tissue repair in carbon tetrachloride hepatotoxicity in male and female Sprague-Dawley and Wistar rats. Int. J. Toxicol., 15, 62–69. doi: 10.3109/10915819609008707.
Cherkashina, D.V., & Petrenko, A.Y. (2006). Hepatopro-tective effect of fetal tissue cytosol and its thermosta-ble fraction in rats with carbon tetrachloride-induced hepatitis. B. Exp. Biol. Med., 141(4), 544–547. doi: 10.1007/s10517-006-0216-y.
Abragamovich, O.O., Grabovska, O.I., & Terletska, O.I. (2000). Protsesy lipidnoyi peroksidatsiyi pry hronichnyh urazhennyah pechinky. Medichna himiya, 2(1), 5–8 (in Ukrainian).
Hariv, M.I., & Gutyj, B.V. (2016). Influence of the lipo-somal preparation Butaintervite on protein synthesis function in the livers of rats under the influence of carbon tetrachloride poisoning. Visnyk of Dniprope-trovsk University. Biology, medicine, 7(2), 123–126. doi: 10.15421/021622.
Sato, S., Dai, W., Liu, X.-L., Asano, G. (1999). The protec-tive effect of hepatocyte growth-promoting factor (pHGF) against carbon tetrachloride-induced acute liver injury in rats: An ultrastructural study. Med. Elec-tron Microsc., 32(3), 184–192. doi: 10.1007/s007950050026.
Yatsenko, O.Iu., & Maloshtan, O.L. (2004). Vplyv ry-biflanu na funktsionalnyi stan pechinky shuriv pry khronichnomu urazhenni tetrakhlormetanom. Visnyk farmatsii, 1(37), 67–70 (in Ukrainian).
Batakov, E.A. (2001) Effect of Silibum marianum oil and legalon on lipid peroxidation and liver antioxidant systems rats intoxicates with carbon tetrachloride. Eksp. Klin. Farmakol., 64(4), 53–55. https://www.ncbi.nlm.nih.gov/pubmed/11589112.
Gutyj, B.V., Murs'ka, S.D., Gufrij, D.F., Hariv, I.I., Levkivs'ka, N.D., Nazaruk, N.V., Gajdjuk, M.B., Pryjma, O.B., Bilyk, O.Ja., & Guta, Z.A. (2016). Influ-ence of cadmium loading on the state of the antioxi-dant system in the organism of bulls. Visnyk of Dnipropetrovsk University. Biology, ecology, 24(1), 96–102. doi: 10.15421/011611.
Gutyj, B., Lavryshyn, Y., Binkevych, V., Binkevych, O., Paladischuk, О., Strons'kyj, J., & Hariv, I. (2016). In-fluence of «Metisevit» on the activity of enzyme and nonenzyme link of antioxidant protection under the bull’s body cadmium loading. Scientific Messenger LNUVMBT named after S.Z. Gzhytskyj, 18, 2(66), 52–58. doi: 10.15421/nvlvet6612.
Gutyj, B., Paska, M., Levkivska, N., Pelenyo, R., Nazaruk, N., & Guta, Z. (2016). Study of acute and chronic toxicity of ‘injectable mevesel’ investigational drug. Biological Bulletin of Bogdan Chmelnitskiy Me-litopol State Pedagogical University, 6(2), 174–180. doi: 10.15421/201649.
Gutyj, B.V., Hufriy, D.F., Hunchak, V.M., Khariv, I.I., Levkivska, N.D., & Huberuk, V.О. (2016). The influ-ence of metisevit and metifen on the intensity of lipid per oxidation in the blood of bulls on nitrate load. Sci-entific Messenger LNUVMBT named after S.Z. Gzhytskyj, 18, 3(70), 67–70 doi: 10.15421/nvlvet7015.
Khariv, M., Gutyj, B., Butsyak, V., & Khariv, I. (2016). Hematological indices of rat organisms under condi-tions of oxidative stress and liposomal preparation ac-tion. Biological Bulletin of Bogdan Chmelnitskiy Meli-topol State Pedagogical University, 6(1), 276–289. doi: 10.15421/201615.
Martyshuk, T.V., Gutyj, B.V., & Vishchur, O.I. (2016). Level of lipid peroxidation products in the blood of rats under the influence of oxidative stress and under the action of liposomal preparation of ‘‘Bu-taselmevit’’, Biological Bulletin of Bogdan Chmelnitskiy Melitopol State Pedagogical University, 6 (2), 22–27. doi: 10.15421/201631.
Huberuk, V., Gutyj, B., Gufriy, D., Binkevych, V., Hariv, I., Binkevych, O., & Salata, R. (2017). Impact of an-tioxidants on enzym activities of glutatione system of bulls bodies antioxidant defense under acute nitrate and nitrite toxicity. Scientific Messenger LNUVMBT named after S.Z. Gzhytskyj, 19(77), 220–224. https://nvlvet.com.ua/index.php/journal/article/view/1212.
Lavryshyn, Y.Y., Varkholyak, I.S., Martyschuk, T.V., Guta, Z.А., Ivankiv, L.B., Paladischuk, О.R., Murska, S.D., Gutyj, B.V., & Gufriy, D.F. (2016). The biological significance of the antioxidant defense system of an-imals body. Scientific Messenger LNUVMBT named after S.Z. Gzhytskyj, 18, 2(66), 100–111. doi: 10.15421/nvlvet6622.
Khariv, M., Gutyj, B., Ohorodnyk, N., Vishchur, O., Khariv, I., Solovodzinska, I., Mudrak, D., Grymak, C., & Bodnar, P. (2017). Activity of the T- and B-system of the cell immunity of animals under conditions of oxidation stress and effects of the liposomal drug. Ukrainian Journal of Ecology, 7(4), 536–541. doi: 10.15421/2017_157.
Lee, J.Y., Lee, J.H., & Kim, H.J. (2004). The preventive inhibition of chondroitin sulfate against the CCI4-induced oxidative stress of subcellular level. Arch. Pharm. Res., 27(3), 340–345. https://www.ncbi.nlm.nih.gov/pubmed/15089041.
Skry`pny`k, I.M. (2007). Gepatoprotetorni zasoby` v suchasnij gepatologiyi. Consilium Medicumllkraina, 1(5), 11–15 (in Ukrainian).
Saba, A.B., Oyagbemi, A.A., & Azeez, O.I. (2010). Ame-lioration of carbon tetrachloride-induced hepatotox-icity and haemotoxicity by aqueous leaf extract of Cnidoscolus aconitifolius in rats. Nig. J. Physiol. Sci., 25(2), 139–147. https://www.ncbi.nlm.nih.gov/ pubmed/22314953.
Antonyak, G.L., Babich, N.O., & Sologub, L.I. (2000). Utvorennya aktivnih form kisnyu ta sistema antioksidantnogo zahistu v organizmi tvarin. Biologiya tvaryn, 2(2), 34–43 (in Ukrainian).
Belenichev, I.F. Levitskiy, E.L., & Kovalenko, S.I. (2002) Antioksidantna sistema zahistu organizmu (oglyad). Sovremennye problemy toksikologii, 3, 29–31 (in Russian).
Gutyj, B., Khariv, I., Binkevych, V., Binkevych, O., Lev-kivska, N., Levkivskyj, D., & Vavrysevich, Y. (2017). Research on acute and chronic toxity of the experi-mental drug Аmprolinsyl. Regulatory Mechanisms in Biosystems, 8(1), 41–45. doi: 10.15421/021708.
Gutyj, B., Grymak, Y., Drach, M., Bilyk, O., Matsjuk, O., Magrelo, N., Zmiya, M., & Katsaraba, O. (2017). The impact of endogenous intoxication on biochemical indicators of blood of pregnant cows. Regulatory Mechanisms in Biosystems, 8(3), 438–443. doi: 10.15421/021768.
Gutyj, B., Martyshchuk, T., Bushueva, I., Semeniv, B., Parchenko, V., Kaplaushenko, A., Magrelo, N., Hirkovyy, A., Musiy, L., & Murska, S. (2017). Mor-phological and biochemical indicators of blood of rats poisoned by carbon tetrachloride and subject to ac-tion of liposomal preparation. Regulatory Mecha-nisms in Biosystems, 8(2), 304–309. doi: 10.15421/021748.
Vlizlo, V.V., Fedoruk, R.S., & Ratych, I.B. (2012). Laboratory methods of investigation in biology, stock-breeding and veterinary. A reference book. Ed. by VV Vlizlo. Lviv: Spolom (in Ukrainian).
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