Pollen analysis included rectum of honey bees
When consuming bees with honey and pollen, the necessary biologically active substances enter into the body. At the same time, in their rectum, during the period of the pituitary gland, a considerable mass of non-permeable remnants accumulate. The main part of them is pollen grains. Covering the issue of forage for wintering bees. Materials for laboratory studies were specimens of honey of different botanical origin, as well as containing rectum honey bees. The article analyzed the possibility of application in the diet of honey bees, except for honey, sugar syrup. Organoleptic evaluation of the main varieties of honey is given. It has been established that consumption of honey of different botanical origin by bees leads to an increase in fecal load during the period of hypobiosis up to 32.0% (P ˂ 0.001). Studies have shown that when consuming sugar syrup in the rectum, isolated pollen grains were detected. With this method of forage can improve the course of physiological processes in the winter. Untreated pollen grains of different honey plants were detected in honey from experimental groups when using honey. However, the dominant number was the grain of honey, which consumed bees in a bleak period. Detailed analysis of the morphological structure of pollen grains isolated from the contents of the rectum of bees during the consumption of such honey: robinium common (acacia), linden small-leaf, rape, sunflower, buckwheat. In 1 g of natural honey contains about 3000 (varies from 60 to 28000) pollen grains of plants, usually 20 species. The largest number of pollen grains is found in buckwheat (about 5–6 thousand in 1 g) of honey, the least in acacia and lime (about 15–20 pcs in 1 g). The pollen grains of entomophilic plants are usually large, sticky, have a pronounced form. The morphological description of pollen grains is carried out at an increase of 1350 times, and measurements – 400 times. To calculate the amount of pollen grains a drop of honey solution is placed on the camera glass Goryaev. The following groups of pollen grains sizes are determined, depending on the length of the axis: very fine pollen grains are 10 microns, small ones are 11–25 microns, medium ones are 26–50 microns, large ones are 51–100 microns, and very large ones are 101–200 microns.
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