Changes in the concentrations of myrystynova end palmitic fatty acids of bee’s pollen during polliniferous season
The article reflects the basic laws of bonds, affecting the production, development of the bee family. To accomplish this, during the 2008–2010 period, Polyphlore sampled pollen in the Kharkiv region, Volchansk district, which is a typical part of the plants and crops of fodder and industrial crops in the Eastern Ukraine. From experimental materials was obtained fatty acid esters. Lipid extraction was conducted by chloroform – methanol mixture (2 : 1), followed by highlights of sodium methylate. Identification of fatty acids was carried out by gas–liquid chromatography with the following operating conditions: Detector temperature – 260 °C, Injector temperature – 250 °C, initial column temperature – 170 °C, final column temperature – 210 °C, carrier gas – nitrogen (flow rate 2 ml / min.). The column is filled with the polar liquid phase (etilenglikolsuktsinat). The results obtained are statistically processed. The spectrum of fatty acids, which attracts bees and is attractants. It is characterized by the presence of meristic, palmitic, oleic, linoleic and linolenic acids. The article traced dynamics of meristic and palmitic fatty acids depending on the collection period. The dynamics of changes in the fatty acid content of bee pollen with seasonal variations and biological characteristics between years. The article describes the impact of these pollen pattern of fatty acids on the development of bee colonies and set the average rate of their needs in the forest–steppe zone every ten days. Obtained regression equations to correct the concentration of these acids. It was found that bee pollen is most enriched with fatty acids in the late spring and early summer (the period of development of bee nests). While analysing the quality of the pollen brought bees it was found that the most intense period of growth of colonies workpiece coincides with the period of pollen with a high level of fatty acids.
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