Using time lapse monitoring of embryo development (TLMED) in reproductive biotechnology

  • M. Sharan Institute of animal biology NAAS
  • S. Shalovylo Lviv National University of Veterinary Medicine and Biotechnologies named after S.Z. Gzhytskyi
  • C. Grymak Institute of animal biology NAAS
Keywords: Time–Lapse, embryo, morphokinetics, assisted reproductive techniques, reproductive biotechnology


An increasing interest in assisted reproductive technologies and their applications in biotechnology of animal reproduction is currently observed. The development of in vitro fertilization (IVF) has led to the emergence of new techniques as ICSI (Intracytoplasmic Sperm Iniection), 1MSI (Morphologically Selected Intracytoplasmic Sperm Iniection) and PGS (Pre–Implantation Genetic Screening). Recently a new technology TLMED (Time Lapse Monitoring of Embryo Development), which allows to observe dynamics of embryo development is being introduced to practice. The application of this technique allows to determine the morphokinetics parameters of normal embryos and to observe its development more accurately. Currently, there are four systems in the market based on this technique: Primo Vision (Vitrolife, Sweden), EEVA (Auxogyn, USA), Embryoscope (Vitrolife, Sweden), MIRI (Esco, Denmark), which conducting the morphokinetics analysis of embryos, which ensures selection of the best embryos and increases the effectiveness of in vitro fertilization. The first positive results of the use of system TLMED in agriculture biotechnology on the example of the Biomedical Research Center of the Warsaw University of Life Sciences predicts its introduction into the everyday practice of veterinary clinics. The aim of this paper is to present each system and review the existing information about the possible application of TLMED and the usefulness in animal reproductive biotechnology.


Payne, D., Flaherty, S.P., Barry, M.F., Matthews C.D. (1997). Preliminary observations on polar body extrusion and pronuclear formation in human oocytes using time–lapse video cinematography. Hum. Reprod. 12, 532–541.

Pribenszky, C., Matyas, S., Kovacs, P. (2010). Preg-nancy achieved by transfer of a single blastocyst selected by time–lapse monitoring. Reprod. Biomed Online. 21, 533–536.

Wong, C.C., Loewke, K.E., Bossert, N.L. (2010). Non–invasive imaging of human embryos before embry-onic genome activation predicts development to the blastocyst stage. Nat. Biotechnol. 28(10), 1115–1121.

Shufaro, Y., Laufer, N. (2013). Epigenetic concerns in assisted reproduction: update and critical review of the current literature. Fertil. Steril. 99(3), 605–606.

Cutting, R., Morroll, D., Roberts, S.A. (2008). Elective single embryo transfer for practice. British Fertility Society and Association of Clinical Embryolo-gists.Hum. Fertil. 11(3), 131–146.

Fisch, J., Rodriguez, H., Ross, R. (2001). The graduated embryo score predicts blastocyst formation and pregnancy rate from cleavage stage embryos. Hum. Reprod. 16, 1970–1975.

Meseguer, M., Rubio, I., Cruz, C. (2012). Embryo incubation and selection in a time–lapse monitoring system improves pregnancy outcome compared with a standard incubator: a retrospective cohort study. Fertil. Steril. 98(6), 1481–1489.

Rubio, I., Kuhlmann, R., Agerholm, I. (2012). Limited implantation success of direct–cleaved human zy-gotes: a time–lapse study. Fertil. Steril. 98(6), 1458–1463.

Wong, C.C., Loewke, K.E., Bossert, N.L. (2010). Non–invasive imaging of human embryos before embry-onic genome activation predicts development to the blastocyst stage. Nat. Biotechnol. 28(10), 1115–1121.

Meseguer, M., Herrero, J., Tejera, A. (2011). The use of morphokinetics as a predictor of embryo implan-tation. Hum. Reprod. 26(10), 2658–2671.

Campbell, A., Fishel, S., Bowman, N. (2013). Model-ling a risk classification of aneuploidy in human embryos using non–invasive morphokinetics. Re-prod. BioMed Online. 26(5), 477–485.

Campbell, A., Fishel, S., Bowman, N. (2013). Retrospective analysis of outcome after using an aneuploidy risk model derived from time–lapse imaging without PCS. Reprod. Biomed. Online. 27(10), 140–146.

Herrero, J., Alberto, T., Ramsing, N.B. (2010). Unking successful implantation with the exact timing of cell division events obtained by time–lapse system in the EmbryoScope. Fertil. Steril. 94(4), 149.

Cruz, M., Perez–Cano, I., Cadea, B. (2011). Time–lapse video analysis provides a correlation between early embryo division kinetics and subsequent blastocyst formation and quality. Hum. Reprod. 26, 115.

Chamayou, S., Patrizio, P., Storaci, C. (2013). The use of morphokinetic parameters to select all embryos with full capacity to implant. J. Assist. Reprod. Genet. 30, 703–710.

Campbell, A., Fishel, S., Duffy, S. (2013). Embryo selection model defined using morphokinetic data from human embryos to predict implantation and live birth. ASRM. 24, 1228.

Basile, N. (2014). Increasing the probability of select-ing chromosomally normal embryos by time–lapse morphokinetics analysis. Fertil. Steril. 101(3), 699–704.

Leibenthron, J., Montag, M., Koster, M. (2012). Influ-ence of age and AMH on early embryo development realised by time–lapse imaging. Hum. Reprod. 27, 135.

Bellver, J., Mifsud, A., Crau, N. (2013). Similar morphokinetic patterns in embryos derived from obese and normoweight infertile women: a time–lapse study. Hum Reprod. 28(3), 794–800.

Ciray, H.N., Aksoy, T., Coktas, C. (2012). Time–lapse evaluation of human embryo development in single versus sequential culture media – a sibling oocyte study. J. Assist. Reprod. Genet. 29(9), 891–900.

Dąbrowski, S., Faundez, R., Petrajtis–Gołobus, M., Gajewski, Z. (2015). Poklatkowa analiza rozwoju zarodka; zastosowanie w biotechnologii rozrodu. Rozrod i mastitis u bydla. Warszawa, 6–13.

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How to Cite
Sharan, M., Shalovylo, S., & Grymak, C. (2016). Using time lapse monitoring of embryo development (TLMED) in reproductive biotechnology. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Agricultural Sciences, 18(2), 274–280.