Using time lapse monitoring of embryo development (TLMED) in reproductive biotechnology


  • M. Sharan Institute of animal biology NAAS
  • S. Shalovylo Lviv National University of Veterinary Medicine and Biotechnologies named after S.Z. Gzhytskyi
  • C. Grymak Institute of animal biology NAAS
Keywords: Time–Lapse, embryo, morphokinetics, assisted reproductive techniques, reproductive biotechnology

Abstract

An increasing interest in assisted reproductive technologies and their applications in biotechnology of animal reproduction is currently observed. The development of in vitro fertilization (IVF) has led to the emergence of new techniques as ICSI (Intracytoplasmic Sperm Iniection), 1MSI (Morphologically Selected Intracytoplasmic Sperm Iniection) and PGS (Pre–Implantation Genetic Screening). Recently a new technology TLMED (Time Lapse Monitoring of Embryo Development), which allows to observe dynamics of embryo development is being introduced to practice. The application of this technique allows to determine the morphokinetics parameters of normal embryos and to observe its development more accurately. Currently, there are four systems in the market based on this technique: Primo Vision (Vitrolife, Sweden), EEVA (Auxogyn, USA), Embryoscope (Vitrolife, Sweden), MIRI (Esco, Denmark), which conducting the morphokinetics analysis of embryos, which ensures selection of the best embryos and increases the effectiveness of in vitro fertilization. The first positive results of the use of system TLMED in agriculture biotechnology on the example of the Biomedical Research Center of the Warsaw University of Life Sciences predicts its introduction into the everyday practice of veterinary clinics. The aim of this paper is to present each system and review the existing information about the possible application of TLMED and the usefulness in animal reproductive biotechnology.

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Published
2016-10-28
How to Cite
Sharan, M., Shalovylo, S., & Grymak, C. (2016). Using time lapse monitoring of embryo development (TLMED) in reproductive biotechnology. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Agricultural Sciences, 18(2), 274–280. https://doi.org/10.15421/nvlvet6760