THE APPLICATION OF THE COMPLEX OF MOLECULAR-GENEIC METHOD FOR IDENTIFICATION OF LACTIC ACID BACTERIA


  • І. M. Slyvka Lviv National University of Veterinary Medicine and Biotechnologies named after S. Z. Gzhytskyj, Lviv
  • O. Y. Tsisaryk Lviv National University of Veterinary Medicine and Biotechnologies named after S. Z. Gzhytskyj, Lviv
  • T. Bocer University of Rzeszow, Kolbuszowa
Keywords: lactic acid bacteria, molecular genetic methods, identification, polymerase chain reaction amplification, heterogeneity

Abstract

Lactic acid bacteria belong to the group of microorganisms, that are basis for creation of preparations of probiotic, that positively influence on a health man. They are widely used in food industry, participating in the process of production and to support of freshness of product and also reduce a requirement in the use at artificial preservatives.

Our purpose was to identify LAB, isolated from ewe’s cheese produced in the alpine mountain valley of Putyla district of Chernivtsi region. the random amplification of polymorphic DNA(RAPD-PCR) and amplification of region of intergenic area of 16S – 23 S rRNA have been used to achieve this purpose.

The analysis of results testifies that bacterial flora that participates in the production of cheese various enough. 8 different genetic profiles that specify on existence of eight groups of bacteria we succeeded to get. Investigated LAB can not be attributed to a specific type because of the lack of similar genetic profiles of reference strains.

The method RAPD-PCR is appropriate in determining the diversity LAB quantitatively, but not sufficient in terms of qualitative analysis. Since lactic flora, which is isolated from sheep cheese Carpathian region of Ukraine, has not been studied, the use of RAPD-PCR method is the first step in its study.

Author Biographies

І. M. Slyvka, Lviv National University of Veterinary Medicine and Biotechnologies named after S. Z. Gzhytskyj, Lviv
аспирант
O. Y. Tsisaryk, Lviv National University of Veterinary Medicine and Biotechnologies named after S. Z. Gzhytskyj, Lviv
д. с/х н., профессор

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Published
2015-04-07
How to Cite
SlyvkaІ. M., Tsisaryk, O. Y., & Bocer, T. (2015). THE APPLICATION OF THE COMPLEX OF MOLECULAR-GENEIC METHOD FOR IDENTIFICATION OF LACTIC ACID BACTERIA. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Agricultural Sciences, 17(1), 201-210. Retrieved from https://nvlvet.com.ua/index.php/agriculture/article/view/3577