Estimation of efficiency of application of technological feed supplement of hepasorbex in vitro
The study of the effectiveness of the application of the technological feed additive Hepasorbex from the functional group of substance in order to reduce the contamination of feed with mycotoxins in laboratory conditions has been carried out. The aim of the work was to establish in vitro the effectiveness of the use of the feed supplements Hepasorbex by confirming its ability to inhibit or reduce the absorption of mycotoxin aflatoxin B1. The effectiveness of the specific action of the feed additive was determined quantitatively at different pH values simulating changes in the acidity of environment in the digestive tract of animals. The study was conducted in two stages: screening – using the solution of mycotoxin and the main – using a forage containing mycotoxin. The feed supplement Hepasorbex was introduced into a buffer solution containing Aflatoxin B1 in an amount of 200 μg/ml at the screening stage. The investigated feed supplement Hepasorbex was introduced into samples of compound feed containing Aflatoxin B1 at a rate of 1, 2, 5 g/kg and carefully homogenized samples in the main experiment. The determination of adsorption (in acidic medium) and desorption (in alkaline medium) was carried out. The criterion for evaluating the effectiveness was the practical efficiency, which was calculated by establishing the difference between the amount of mycotoxin introduced in the buffer solution or the amount of mycotoxin found in feed and the amount of adsorbed mycotoxin after incubation in the acidic environment, taking into account the size of the desorption after incubation in alkaline environment. It was established that in the screening studies, the practical factor of the beneficial effect of the feed supplement on Aflatoxin B1 was 89.5%. It was found in the main study that the practical benefit factor of the feed supplement, depending on the dose, was 75.0–84.4%. The obtained results confirm that the investigated feed additive possesses clear adsorption properties in relation to aflatoxin B1.
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