Estimation of efficiency of application of technological feed supplement of hepasorbex in vitro
Abstract
The study of the effectiveness of the application of the technological feed additive Hepasorbex from the functional group of substance in order to reduce the contamination of feed with mycotoxins in laboratory conditions has been carried out. The aim of the work was to establish in vitro the effectiveness of the use of the feed supplements Hepasorbex by confirming its ability to inhibit or reduce the absorption of mycotoxin aflatoxin B1. The effectiveness of the specific action of the feed additive was determined quantitatively at different pH values simulating changes in the acidity of environment in the digestive tract of animals. The study was conducted in two stages: screening – using the solution of mycotoxin and the main – using a forage containing mycotoxin. The feed supplement Hepasorbex was introduced into a buffer solution containing Aflatoxin B1 in an amount of 200 μg/ml at the screening stage. The investigated feed supplement Hepasorbex was introduced into samples of compound feed containing Aflatoxin B1 at a rate of 1, 2, 5 g/kg and carefully homogenized samples in the main experiment. The determination of adsorption (in acidic medium) and desorption (in alkaline medium) was carried out. The criterion for evaluating the effectiveness was the practical efficiency, which was calculated by establishing the difference between the amount of mycotoxin introduced in the buffer solution or the amount of mycotoxin found in feed and the amount of adsorbed mycotoxin after incubation in the acidic environment, taking into account the size of the desorption after incubation in alkaline environment. It was established that in the screening studies, the practical factor of the beneficial effect of the feed supplement on Aflatoxin B1 was 89.5%. It was found in the main study that the practical benefit factor of the feed supplement, depending on the dose, was 75.0–84.4%. The obtained results confirm that the investigated feed additive possesses clear adsorption properties in relation to aflatoxin B1.
References
Diaz, D.E., & Smith, T.K. (2005). Mycotoxin Sequestering Agents: Practical Tools for the Neutralization of Mycotoxins. The Mycotoxin Blue Book.
Fruhauf, S., Schwartz, H., Ottner, F., Krska, R., & Vekiru, E. (2012). Yeast cell based feed additives: studies on aflatoxin B1 and zearalenone. Food Additives and Contaminants, 29(2), 217–231. doi: 10.1080/19440049.2011.630679.
Golovnja, E. (2014). Nuzhno li ocenivat' jeffektivnost' sorbentov. Kombikorma, 4, 67–68 ( in Russian).
Kocyumbas, I.Ya., Bisyuk, I.Yu., Gorzheyev, V.M., & Maly`k, O.G. (2013). Klinichni doslidzhennya vet-ery`narny`h preparativ ta kormovy`h dobavok. L`viv (in Ukrainian).
Kong, C., Shin, Y., & Gyun, B.K. (2014). Evaluation of mycotoxin sequestering agents for aflatoxin and deoxynivalenol: an in vitro approach. SpringerPlus, 3, 346. doi: 10.1186/2193-1801-3-346.
Levytskyy, T.R. (2018). Efficiency of use technological feed additive. Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnolo-gies, 20(84), 143–147. doi: 10.15421/nvlvet8426.
Lysko, S.B. (2013). Sorbcionnaja aktivnost' novogo sorbenta prirodnogo proishozhdenija v otnoshenii T-2 toksina. Veterinarija i kormlenie, 6, 28–29 (in Rus-sian).
Malkov, M.A., Bogomolov, V.V., Dan'kova, T.V., & Krasnov, K.A. (2012). Mikotoksiny – strategija ustranenija ih vlijanija na organizm sel'skohozjajstvennyh zhivotnyh i pticy. Cenovik, 1, 74–75 (in Russian).
Pappas, A.C. Tsiplakou, E., Georgiadou, M., Anagnostopoulos, C., Markoglou, A.N., Liapis, K., & Zervas, G. (2014). Bentonite binders in the presence of mycotoxins: Results of in vitro preliminary tests and an in vivo broiler trial. Applied Clay Science, 99, 48–53. doi: 10.1016/j.clay.2014.06.009.
Thieu, N., & Pettersson, H. (2008). In vitro evaluation of the capacity of zeolite and bentonite to adsorb aflatoxin B1 in simulated gastrointestinal fluids. Mycotoxin Research, 24(3), 124–129. doi: 10.1007/BF03032338.
Vekiru, E., Fruhauf, S., Sahin, M., Ottner, F., Schatzmayr, G., & Krska, R. (2007). Investigation of various adsorbents for their ability to bind Aflatoxin B1 Mycotoxin Research, 23(1), 27–33. doi: 10.1007/BF02946021.
Zakon Ukrayiny` “Pro bezpeku ta gigiyenu kormiv”, Golos Ukrayiny`, № 12, 19.01.2018 (in Ukrainian).
Abstract views: 82 PDF Downloads: 98