Hydrocarbon metabolism in mice during preclinical studies of modified starch


Keywords: liver, serum, modified starch, laboratory animals, glycogen, glucose, lactic acid, pyruvic acid

Abstract

Preclinicl studies of new nutritional supplements include the establishment of not only harmlessness, lethal or semi-lethal dose, but also biochemical signs of the laboratory animals at certain doses of the substance. New food additives include modified starch. Of scientific interest are the studies of white mice hydrocarbon metabolism considering the safety of modified starch. The study of modified starch safety was performed according to the established requirements. Eighteen 2-month-old laboratory mice (females) were used for the study, with an average body weight of 19–21 g. Groups were formed using the randomization method. Each group consisted of six experimental animals. Experiments on the harmlessness of modified starch on white mice were performed according to the scheme, where animals from the control group were intramuscularly administered 0.3 cm3 of 0.9% NaCI solution. Mice from the first experimental group were led with a 5.0% solution of modified starch. Animals from experimental II were guided by a 10.0% solution of modified starch. The volume of solutions of modified starch was 0.3 cm3. Blood serum of laboratory mice was tested for glucose, lactic and pyruvic acid levels, and the liver was used to determine glycogen level. All those actions were performed according to current bioethics requirements. It was experimentally proven that on the eleventh day from the beginning of injecting the 0.3 cm3 5.0% and 10.0% solution of modified starch in the blood serum of the laboratory mice glucose content did not differ from those obtained in control animals, which were injected with saline. When using the test solutions, itt was found that the levels of lactic and pyruvic acid in the serum of experimental animals were within the physiological standards. In those mice that were injected intragastrically the increased doses of modified starch the level of glycogen in the liver was about 39.740.1 g/kg which corresponded to physiological standards. Thus, a single injection of 0.3 cm3 5.0% and 10.0% solution of modified starch does not cause disorder of hydrocarbon metabolism in mice.

References

Nafikov, R., & Beitz, D. (2007). Carbohydrate and Lipid Metabolism in Farm Animals. Journal of Nutrition, 137(3), 702–705. doi: 10.1093/jn/137.3.702.

Aschenbach, J.R., Kristensen, N.B., Donkin, S.S., Hammon, H.M., & Penner, G.B. (2010). Gluconeo-genesis in dairy cows: The secret of making sweet milk from sour dough. IUBMB Life, 62(12), 869–877. doi: 10.1002/iub.400.

De Koster, J.D., & Opsomer, G. (2013). Insulin resistance in dairy Cows. Vet Clin North Am Food Anim Pract., 29(2), 299–322. doi: 10.1016/j.cvfa.2013.04.002.

Gorjacheva, M.A., & Makarova, M.N. (2016). Osoben-nosti provedenija gljukozotolerantnogo testa u melkih laboratornyh gryzunov (myshi i krysy). Mezhdu-narodnyj vestnik veterinarii, 3, 155–160. https://elibrary.ru/item.asp?id=26740182 (in Russian).

Cody, G.D., Boctor, N.Z., Filley, T.R. et al. (2000). Pri-mordial Carbonylated Iron-Sulfur Compounds and the Synthesis of Pyruvate. Science New Series, 289, 1337–1340. doi: 10.1126/science.289.5483.1337.

Sharkey, L.C., & Wellman, M.L. (2015). Use of Lactate in Small Animal Clinical Practice. Clin Lab Med., 35(3), 567–577. doi: 10.1016/j.cll.2015.05.006.

Di Mauro, F.M., & Schoeffler, G.L. (2016). Point of Care Measurement of Lactate. Top Companion Anim Med., 31(1), 35–43. doi: 10.1053/j.tcam.2016.05.004.

Goodwin, M.L., Harris, J.E., Hernández, A., & Gladden, L.B. (2007). Blood lactate measurements and analy-sis during exercise: a guide for clinicians. Journal of Diabetes Science and Technology, 1(4), 558–569. doi: 10.1177/193229680700100414.

Katunina, N.P., Stratienko, E.N., & Ceeva, F.N. (2015). Vlijanie novyh himicheskih soedinenij, ostroj gipoksii s giperkapniej i ih sochetannogo dejstvija na nekotorye pokazateli glikoliticheskogo puti obmena uglevodov u myshej. Vestnik Brjanskogo gosudarstvennogo universiteta, 39–40 (in Russian).

Offermanns, S., Colletti, S.L., & Lovenberg, T.W. (2011). International Union of Basic and Clinical Pharmacol-ogy. LXXXII: Nomenclature and Classification of Hydroxy-carboxylic Acid Receptors (GPR81, GPR109A, and GPR109B). Pharmacological Reviews, 63(2), 269–290. doi:10.1124/pr.110.003301.

Offermanns, S., Colletti, S.L., & IJzerman, A.P. (2018). Hydroxycarboxylic acid receptors. IUPHAR/BPS Guide to Pharmacology. International Union of Basic and Clinical Pharmacology, 13.

Roach, P.J., Depaoli-Roach, A.A., Hurley, T.D., & Taglia-bracci, V.S. (2012). Glycogen and its metabolism: some new developments and old themes. Biochem J., 441(3), 763–787. doi: 10.1042/BJ20111416.

Garverick, H.A., Harris, M.N., & Vogel-Bluel, R. et al. (2013). Concentrations of nonesterified fatty acids and glucose in blood of periparturient dairy cows are indicative of pregnancy success at first insemination. J Dairy Sci., 96(1), 181–188. doi: 10.3168/jds.2012-5619.

Izumida, Y., Yahagi, N., Takeuchi, Y. et al. (2013). Gly-cogen shortage during fasting triggers liver–brain–adipose neurocircuitry to facilitate fat utilization. Na-ture Communications, 4, 2316. doi: 10.1038/ncomms3316.

Chechetkin, A.V., Voronjanskij, V.I., Pokusaj, G.G., Kartashov, N.I., Doktorovich, N.L., & Kirichenko, I.V. (1980). Praktikum po biohimii sel'skohozjajstvennyh zhivotnyh. M.: Vysshaja shkola (in Russian).

Kushnir, O.Ju. (2010). Vplyv melatoninu na pokaznyky vuglevodnogo obminu v shhuriv z aloksanovym diabetom: avto- ref. dys. na zdobuttja nauk. stupenja kand. med nauk: spec. 03.00.04 “Biohimija”. Instytut gerontologii' im. D.F. Chebotar'ova. K. (in Ukrainian).

Instrukcija do naboru reaktyviv dlja vyznachennja gljukozy v biologichnyh ridynah po kol'orovij reakcii' z orto-tolui'dynovym reaktyvom (kat. № NR009.01). Zatverdzhena Instytutom hirurgii' ta transplantologii' AMN Ukrai'ny vid 10 zhovtnja 2003 r. K., 2003, 2 (in Ukrainian).

Plohinskij, N.A. (1969). Rukovodstvo po biometrii dlja zootehnikov. Moskva: Kolos (in Russian).

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Published
2019-11-06
How to Cite
Vovkohon, A. (2019). Hydrocarbon metabolism in mice during preclinical studies of modified starch. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Agricultural Sciences, 21(91), 33-36. https://doi.org/10.32718/nvlvet-a9106