The influence of citrates Ag and Cu on the lipid composition of the tissues of the honey bees body and bee bread under the condition of their added to the feeding in the spring period


Keywords: honey bee, bee bread, lipids, silver and copper nanoparticles citrate-based

Abstract

Experimental data of the lipid composition of the honey bees and bee bread in the spring period under the conditions of feeding sugar syrup with Ag and Cu citrates are presented in the article. The studies were conducted on 3 groups of bee colonies, three bee colonies each. Control group (I) received sugar syrup at a concentration of 1:1 (1000 ml per week), experimental (II) group received under similar conditions with sugar syrup 0.2 mg of Argentum and 0.2 mg of Cuprum in citrate form. The third experimental group was fed with sugar syrup Ag and Cu citrates at doses of 0.5 mg each per 1000 ml of sugar syrup per week. The study was lasted 36 days. Samples of tissues of the whole organism of honey bees in the amount of 30–35 pieces were selected for the study. from each bee colony and bee bread to determine the total lipid content by the Folch method and their separate fractions: phospholipids, mono- and diacylglycerols, free cholesterol, non-esterified fatty acids, triacylglycerols, esterified cholesterol glycol cholesterol using thin layer chromatography using silica gel plates Sorbfil. According to the results of the research, we found intergroup differences of total lipid content in the tissues of whole organism of the honey bees and bee bread of experimental groups in comparison with the control group. Higher levels of phospholipids, esterified cholesterol, and lower levels of free cholesterol, mono- and diacylglycerols and triacylglycerols were found in the tissue of bees II and III of the experimental group. Introduction of Ag and Cu citrates into the components of bee feeding in the bee bread the level of phospholipids (P ˂ 0.01), mono and diacylglycerols (group III), triacylglycerols increased against the decrease of free cholesterol (P ˂ 0.05) and esterified cholesterol (P ˂ 0.01).

References

Alaux, C., Ducloz, F., Crauser, D., & Le Conte, Y. (2010). Diet effects on honeybee immunocompetence. Biol. Lett., 6(4), 562–565. doi: 10.1098/rsbl.2009.0986.

Anđelković, B., Jevtić, G., Mladenović, M., Petrović, M., & Vasić, T. (2011). Influence of spring feed on the strength of honey bee colonies during spring develop-ment. Biotechnology in Animal Husbandry, 27(4), 1757–1760. doi: 10.2298/BAH1104757A.

Chen, H. (2018). Metal based nanoparticles in agricultur-al system: behavior, transport, and interaction with plants. Chemical Speciation & Bioavailability, 30(1), 123–134. doi: 10.1080/09542299.2018.1520050.

Fedoruk, R.S., & Romaniv, L.I. (2014). Umist zahalnykh lipidiv u tkanynakh medonosnykh bdzhil za zghod-ovuvannia boroshna z bobiv natyvnoi ta henetychno modyfikovanoi soi. Visnyk ahrarnoi nauky, 1(731), 30–32 (in Ukrainian).

Fedoruk, R.S., & Romaniv, L.I. (2014). Vmist zahalnykh lipidiv i spivvidnoshennia yikh fraktsii u tkanynakh medonosnykh bdzhil za zghodovuvannia boroshna natyvnoi soi z dodavanniam khlorydu ta tsytratu khromu. Biolohiia tvaryn, 16(1), 160–168 (in Ukrain-ian).

Feregrino-perez, A.A., Magaña-lópez, E., Guzmán, C., & Esquivel, K. (2018). A general overview of the benefits and possible negative effects of the nanotechnology in horticulture. Sci. Hortic., 238, 126–137. doi: 10.1016/j.scienta.2018.03.060.

Hladun, K., Di, N., Liu, T.X., & Trumble, J. (2016). Metal contaminant accumulation in the hive: consequences for whole colony health and brood production in the honey bee (Apis mellifera L.). Environ. Toxicol. Chem., 35(2), 322–329. doi: 10.1002/etc.3273.

Kalashnikova, M.V., & Sidorova, K.A. (2016). Vozmozhnosti povyshenija bioresursnogo potenciala pchel. Molodoj uchenyj, 12, 1083–1085 (in Russian).

Kather, R., Drijfhout, F.P., & Martin, S.J. (2011). Task group differences in cuticular lipids in the honey bee Apis mellifera. Journal of Chemical Ecology, 37(2), 205–212. doi: 10.1007/s10886-011-9909-4.

Kovalska, L.M., Kyryliv, Ya.I., & Kovalskyi, Yu.V. (2009). Lipidnyi sklad okremykh tkanyn medonosnykh bdzhil u vikovomu aspekti. Naukovo-tekhnichnyi biuleten Instytutu biolohii tvaryn Derzhavnoho naukovo-doslidnoho kontrolnoho insty-tutu vetpreparativ ta kormovykh dobavok. Lviv, 10(1–2), 51 (in Ukrainian).

Larionova, O.S. (2005). Dinamika soderzhanija zhira v organizme rabochih pchel v uslovijah estestvennoj sredy pri razlichnyh vidah podkormok i masse otvod-kov. Sovremennye problemy intensifikacii proizvod-stva v APK: tr. Vseros. NII kontrolja standartizacii i sertifikacii veterinarnyh preparatov, 88–89 (in Rus-sian).

Madras–Majewska, B., Zygmunt, Ja., & Gąbka, J. (2009). Effect of early supplemental feeding honey-bee colonies with a substiute of bee bread made of pollen and honey on colony strengh. Naukovyi visnyk LNUVMBT imeni S.Z. Gzhytskoho, 11, 3(42), 337–341.

Mamonova, I.A., Matasov, M.D., Babushkina, I.V., Lo-sev, O.Je., Chebotareva, E.G., Gladkova, E.V., & Bo-rodulina, E.V. (2013). Izuchenie fizicheskih svojstv i biologicheskoj aktivnosti nanochastic medi. Rossijs-kie nanotehnologii, 8(5–6), 25–29 (in Russian).

Manning, R. (2016). Artificial feeding of honeybees based on an understanding of nutritional principles. Animal Production Science, 58(4), 689–703. doi: 10.1071/AN15814.

Manning, R., Rutkay, A., Eaton, L., & Dell, B. (2007). Lipid-enhanced pollen and lipid-reduced flour diets and their effect on the longevity of honeybees (Apis mellifera L.). Australian Journal of Entomology, 46, 251–257. doi: 10.1111/j.1440-6055.2007.00598.x.

Pashchenko, A.H., Kovalchuk, I.I., & Romaniv, L.I. (2017). Vmist zahalnykh lipidiv i spivvidnoshennia okremykh yikh klasiv u tkanynakh orhanizmu ta produktsii medonosnykh bdzhil za umov pidhodivli tsytratamy kobaltu ta nikeliu. Visnyk Zhytomyrskoho natsionalnoho ahroekolohichnoho universytetu. Zhy-tomyr, 3, 2(63), 143–148 (in Ukrainian).

Rai, M., Kon, K., Ingle, A., Duran, N., Galdiero, S., & Galdiero, M. (2014). Broad-spectrum bioactivities of silver nanoparticles: the emerging trends and future prospects. Applied Microbiology and Biotechnology, 98(5), 1951–1961. doi: 10.1007/s00253-013-5473-x.

Romaniv, L.I., Kovalchuk, I.I., Fedoruk, R.S., & Pash-chenko, A.H. (2018). Umist lipidiv u tkanynakh orhanizmu medonosnykh bdzhil za zghodovuvannia boroshna soi, tsukrovoho syropu i tsytrativ Co ta Ni. Biolohiia tvaryn, 20(3), 84–92 (in Ukrainian).

Samantarai, S.K., & Achakzai, A.K.K. (2014). Applica-tion of nanotechnology in agriculture and food pro-duction: opportunity and challenges. Middle-East J. Sci., 22, 499–501.

Sharma, V.K., Siskova, K.M., Zboril, R. et al. (2014). Organic-coated silver nanoparticles in biological and environmental conditions: fate, stability and toxicity. Adv Colloid Interface Sci., 204, 15–34. doi: 10.1016/j.cis.2013.12.002.

Sinclair, B.J., & Marshall, K.E. (2018). The many roles of fats in overwintering insects. Journal of Experimental Biology, 221: jeb161836. doi: 10.1242/jeb.161836.

Trofimova, S.A. (2015). Metodologicheskie podhody k ocenke biologicheskogo dejstvija nanomaterialov. Journal of Biomedical Technologies, 1, 38–44. doi: 10.15393/j6.art.2015.3283 (in Russian).

Ulutaş, K., & Özkirim, A. (2018). Importance of Nutrition for Honey Bee Health. Mellifera, 18(1), 30–35. https://dergipark.org.tr/en/download/article-file/586520.

Zarić, N.M., Ilijević, K., Stanisavljević, L, & Gržetić, I. (2016). Metal concentrations around thermal power plants, rural and urban areas using honeybees (Apis mellifera L.) as bioindicators. International Journal of Environmental Science and Technology, 13(2), 413–422. doi: 10.1007/s13762-015-0895-x.

Abstract views: 0
PDF Downloads: 0
Published
2019-11-06
How to Cite
Dvylyuk, I., & Kovalchuk, I. (2019). The influence of citrates Ag and Cu on the lipid composition of the tissues of the honey bees body and bee bread under the condition of their added to the feeding in the spring period. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Agricultural Sciences, 21(91), 123-127. https://doi.org/10.32718/nvlvet-a9122