Experimental evaluation of the acute toxicity and irritant effect of “BTF plus” – a veterinary medicinal product for the normalization of metabolic processes in animals and poultry

R. M. Sachuk; B. V. Gutyj; T. A. Velesyk; S. M. Lyko; O. A. Katsaraba; V. O. Pepko; O. I. Portukhai; O. O. Yakuta

doi:10.32718/nvlvet-a9903

R. M. Sachuk Rivne State University of the Humanities, Rivne, Ukraine https://orcid.org/0000-0003-4532-4220
B. V. Gutyj Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies, Lviv, Ukraine https://orcid.org/0000-0002-5971-8776
T. A. Velesyk Rivne State University of the Humanities, Rivne, Ukraine https://orcid.org/0000-0003-3201-9323
S. M. Lyko Rivne State University of the Humanities, Rivne, Ukraine https://orcid.org/0000-0002-0470-4836
O. A. Katsaraba Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies, Lviv, Ukraine https://orcid.org/0000-0001-8520-224X
V. O. Pepko Ternopil research station of the Institute of Veterinary Medicine of the National Academy of Sciences, Ternopil, Ukraine https://orcid.org/0000-0003-2687-1958
O. I. Portukhai Rivne State University of the Humanities, Rivne, Ukraine https://orcid.org/0000-0002-9078-0658
O. O. Yakuta Rivne State University of the Humanities, Rivne, Ukraine

DOI: https://doi.org/10.32718/nvlvet-a9903

Keywords: “BTF plus”, mice, rats, acute toxicity, dose, lethality, toxicity.

Abstract

Laboratory studies were conducted to determine the acute toxicity of the veterinary drug “BTF plus” on white rats and white mice. The preparation “BTF plus” is a complex vitamin and mineral preparation, which is used to normalize and correct metabolic processes in poultry and animals. The drug is used for various types of animals and poultry as a stimulating, tonic and general strengthening agent for: obstetric pathologies (difficult births, postpartum complications, paresis, eclampsia, sexual cycle disorders); metabolic disorders caused by irrational feeding, malnutrition, asthenic syndrome, etc.; anemia with helminthiasis; secondary anemias, as an additional means in the treatment of magnesium and calcium deficiency; to increase muscle activity, with significant loads, overstrain and exhaustion in animals; to increase the body’s resistance to various pathogens; to stimulate growth, development and live weight gain in young animals; as an additional means in the treatment of diseases caused by various factors (infectious and non-infectious origin). Based on the results of determining the parameters of the acute toxicity of the drug “BTF plus” in the case of a single intragastric administration to white female rats, it was not possible to calculate the LD₅₀, since the death of laboratory animals was not detected within 14 days after administration. At the same time, the maximum injected dose (based on the absolute weight of the drug) was 40000.0 mg/kg of body weight, which allows the drug to be classified as VI class of toxicity – the substances are relatively harmless (LD₅₀> 15000.0 mg/kg of body weight), and according to the degree of danger to IV class – low-hazardous substances (LD₅₀> 5000.0 mg/kg of body weight). Based on the results of determining the parameters of the acute toxicity of the drug “BTF plus” in the case of a single subcutaneous injection to white female rats and male mice, it was not possible to calculate the LD₅₀, since the death of laboratory animals was not detected within 14 days after administration. At the same time, the maximum injected dose (based on the absolute weight of the drug) was 20000,0 and 40000.0 mg/kg of body weight for both species of animals, respectively, which allows it to be classified as VI class – relatively harmless substances (LD_50Subcut > 4500 mg/kg of body weight). Further studies will be the next stage of pre-registration tests aimed at studying the embryotoxic effect of “BTF plus”, which is mandatory material of the “Safety and residue studies” section of the dossier for this drug.

References

Council Directive 86/609/EEC of 24 November 1986 on the approximation of laws, regulations and adminis-trative provisions of the Member States regarding the protection of animals used for experimental and other scientific purposes. Offical Journal of the European Communities L 358. 1986. Р. 1–29. URL: https://op.europa.eu/en/publication-detail/-/publication/cc3a8ccb-5a30-4b6e-8da8-b13348caeb0c/language-en.

European convention for the protection of vertebrate animals used for experimental and other scientific purposes. Council of Europe. Strasbourg, 1986. 53 p. URL: https://rm.coe.int/168007a67b.

Gutyj, B., Khariv, I., Binkevych, V., Binkevych, O., Lev-kivska, N., Levkivskyj, D., & Vavrysevich, Y. (2017). Research on acute and chronic toxity of the experi-mental drug Аmprolinsyl. Regulatory Mechanisms in Biosystems, 8(1), 41–45. DOI: 10.15421/021708.

Gutyj, B., Martyshuk, T., Jankowski, M., Karpovskyi, V., & Postoi, R. (2022). Effect of the Feed Additive Bu-taselmevit-Plus on the Antioxidant Status of the Rat Body Due to Cadmium and Lead Intoxication. Ukrainian Journal of Veterinary Sciences, 13(2), 9–15. DOI: 10.31548/ujvs.13(2).2022.9-15.

Hunchak, Yu. R., Gutyj, B. V., Sachuk, R. M., & Stravsky, Ya. S. (2020). Study of the parameters of acute toxicity of the drug “Devimectin 1 %” with a single subcutaneous injection in white rats. Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnologies. Series: Veterinary sci-ences, 22(100), 28–31. DOI: 10.32718/nvlvet10005.

Katsaraba, O. A., Sachuk, R. M., Gutyj, B. V., Velesyk, T. A., Radzykhovskyi, M. L., Sharandak, P. V., & Pepko, V. O. (2022). Pharmacological studies of the veteri-nary medicinal product “Dibutalastin Ointment”. Ukrainian Journal of Veterinary and Agricultural Sci-ences, 5(2), 43–48. DOI: 10.32718/ujvas5-2.07.

Katsaraba, O. A., Sachuk, R. M., Stravskyi, Ya. S., & Kostyshyn, L.-M. Ye. (2021). Novyi vitaminno-mineralnyi preparat dlia profilaktyky akusherskoi patolohii tvaryn “Biotan 3Z” [New vitamin-mineral preparation for the prevention of obstetric pathology of animals “Biotan 3Z”]. Naukovo-tekhnichnyi biuleten Derzhavnoho naukovo-doslidnoho kontrolnoho instytutu veterynarnykh preparativ ta kormovykh dobavok i Instytutu biolohii tvaryn, 22(2), 141–148. DOI: 10.36359/scivp.2021-22-2.17.

Kotsiumbas, I. Ia., Malyk, O. H., Patereha, I. P. ta in. (2006). Doklinichni doslidzhennia veterynarnykh likarskykh zasobiv [Preclinical studies of veterinary medicinal products]. Lviv: Triada plius (in Ukrainian).

Martyshuk, T. V., Gutyj, B. V., & Khalak, V. I. (2021). System of antioxidant protection of the body of pig-lets under the action of feed additive “Butaselmevit-plus”. Ukrainian Journal of Veterinary and Agricultur-al Sciences, 4(2), 38–43. DOI: 10.32718/ujvas4-2.07.

Martyshuk, T., Gutyj, B., Vyshchur, O., Paterega, I., Kushnir, V., Bigdan, O., et al. (2022). Study of Acute and Chronic Toxicity of “Butaselmevit” on Laborato-ry Animals. Arch Pharm Pract, 13(3), 70–75. DOI: 10.51847/XHwVCyfBZ3.

Sachuk, R. M. (2019). Determination of the vitamin-mineral preparation “Energolit” stability for the treatment of metabolic disorders in animals. Journal for Veterinary Medicine, Biotechnology and Biosafe-ty, 5(1), 10–13. URL: http://jvmbbs.kharkov.ua/archive/2019/volume5/issue1/oJVMBBS_2019051_010-012.pdf.

Sachuk, R. M., Gutyj, B. V., Velesyk, T. A., Stravskyy, Y. S., Katsaraba, O. A., Pepko, V. O., & Vasiv, R. O. (2022). Effectiveness of the drug Kolidev 8M (powder for oral use) for the treatment of bacterial infections in decorative birds and European fallow deer. Theo-retical and Applied Veterinary Medicine, 10(3), 3‒12. DOI: 10.32819/2022.10011.

Sachuk, R. M., Zhyhaliuk, S. V., Stravskyi, Ya. S., & Chaikovska, O. I. (2019). Novyi mineralnyi preparat dlia veterynarnoi praktyky “Kalfomin” [New mineral preparation for veterinary practice “Kalfomin”]. Nau-kovo-tekhnichnyi biuleten Derzhavnoho naukovo-doslidnoho kontrolnoho instytutu veterynarnykh pre-parativ ta kormovykh dobavok i Instytutu biolohii tvaryn, 20(2), 390–399. DOI: 10.36359/scivp.2019-20-2.50.

Sachuk, R., Stravskyy, Y., Gutyj, B., Velesyk, T., Katsaraba, O., & Zhyhaliuk, S. (2021). Study of acute toxicity of the drug “Kolidev 8M” with a single intra-gastric injection in laboratory animals. ScienceRise: Biological Science, 2(27), 44–48. DOI: 10.15587/2519-8025.2021.235952.

Varkholiak, I. S., & Gutyj, B. V. (2018). Determination of acute toxicity of “Bendamin” drug in laboratory ani-mals. Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnologies, 20(92), 209–212. DOI: 10.32718/nvlvet9243.

Varkholiak, I. S., Gutyj, B. V., Gufriy, D. F., Sachuk, R. M., Mylostyvyi, R. V., Radzykhovskyi, M. L., Sedilo, H. M., & Izhboldina, O. O. (2021). The effect of the drug “Bendamine” on the clinical and morphological parameters of dogs in heart failure. Ukrainian Journal of Veterinary and Agricultural Sciences, 4(3), 76–83. DOI: 10.32718/ujvas4-3.13.

Experimental evaluation of the acute toxicity and irritant effect of “BTF plus” – a veterinary medicinal product for the normalization of metabolic processes in animals and poultry

Abstract

References

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