Non-waste biotechnology of obtaining a symbiotic and a metabolite on the basis of Bifidobacterium longum – Ya 3 and Propionibacterium shermanii – 4


Keywords: propionic acid bacteria, bifidobacteria, supernatant, metabolites, probiotic

Abstract

In order to eliminate dysbiotic violations of microbiota of the human gastrointestinal tract probiotics are used. However, in recent years data has been accumulated on the reduction of the effectiveness of classical probiotics, especially against antibiotic therapy, so a promising group of probiotics of a metabolic type is gaining popularity. Metabiotics contain metabolic products or structural components of probiotic microorganisms. The purpose of experimental work was to develop a technology of non-waste production using a culture fluid of probiotic microorganisms as raw material for the creation of a cell-free probiotic. The objects of the study were cultures of microorganisms of the Museum of the Department of Biochemistry, Microbiology and Nutrition Physiology ONAFT Вifidobacterium longum – Ya 3, Propionibacterium shermanii – 4, the culture fluid of these probiotic strains. Subject of research - structural elements of metabolic activity of probiotic microorganisms, organoleptic, physico-chemical and microbiological indices. After co-cultivating B. longum – I 3 + P. shermanii-PS 4 in a lactose medium with the addition of soy serum at a temperature of (34 ± 1) °C for 24 hours with a titre of at least 1 1010 CFU/cm3, a culture medium supernatant was obtained by centrifugation at 8000 rpm for 20 minutes and further filtration because of bacterial filters in aseptic conditions. The bifidogenic growth stimulator was purified from the microbial biomass residue by vacuum filtration using bacterial filters (Millipore, 0.22 μm). Its determination was carried out by gas-liquid chromatography using the GC-16A «Shimadzu» (Japan) gas chromatograph with the possibility of temperature programming up to 330 °C, flame-ionization detector and software «GC solution». The content of 1,4-dihydroxy-2-naphthoic acid in the supernatant of the consortium Вifidobacterium longum – Ya 3, Propionibacterium shermanii – 4 in the amount of 4.1 mg/liter was determined. The principle of technological schemes of non-waste production of biologically active additives based on the classical and metabolic probiotic developed.

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Published
2018-03-02
How to Cite
Krupytska, L., Kaprelyants, L., Kylymenchuk, O., & Velichko, T. (2018). Non-waste biotechnology of obtaining a symbiotic and a metabolite on the basis of Bifidobacterium longum – Ya 3 and Propionibacterium shermanii – 4. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Food Technologies, 20(85), 148-154. https://doi.org/10.15421/nvlvet8527