Features of the enzyme composition of Jerusalem artichoke tubers


Keywords: Jerusalem artichoke, oxidizing enzymes, oxidoreductases, polyphenol oxidase, peroxidase, ascorbate oxidase, ascorbic acid, microwave processing

Abstract

The article deals with different methods of preparing Jerusalem artichoke tubers to stabilize the color of semi-finished products and finished products. The varieties Interés and Violet de Rennes were chosen as objects of study. The purpose of this work is to study the activity of the enzyme system of Jerusalem artichoke tubers, followed by the use of raw materials as an ingredient in the preparation of culinary dishes with high nutritional and biological value. In accordance with the objective, the characteristics of the enzymatic composition of the material under study were researched. It was found that of the oxidoreductases present, the most active enzyme is polyphenol oxidase. It has been established that in the tubers of Jerusalem artichoke, the activity of polyphenol oxidase is in 1.8 times lower, peroxidase – in 1.34 times higher; ascorbate oxidase is in 2.79 times higher than that of Violet de Rennes tubers. A comparative analysis was performed on the reduction of the activity of enzymes and the preservation of the mass fraction of L-ascorbic acid in heat treatment and microwave processing. It has been established that the most successful method of treating Jerusalem artichoke tubers in order to inactivate the enzyme system and to preserve L-ascorbic acid is a microwave processing of 600 W in 1 minute. Studies have shown that with such preparation, the semi-finished product does not change color, the activity of the enzyme polyphenol oxidase decreases by 20 times, and the maintenance of the content of L-ascorbic acid is 68.4%. In further studies, it is important to study the technologies for preparing culinary dishes based on Jerusalem artichoke tubers using the results obtained to stabilize the color and preserve the biologically active substances of the raw materials.

References

Bach, V., Clausen, M., & Edelenbos, M. (2015). Produc-tion of Jerusalem Artichoke (Helianthus tuberosus L.) and Impact on Inulin and Phenolic Compounds. Pro-cessing and Impact on Active Components in Food, 6, 97–102. doi: 10.1016/B978-0-12-404699-3.00012-3.

Bach, V., Jensen, S., Clausen, M.R., & Bertram, H.C. (2013). Enzymatic browning and after-cooking dark-ening of Jerusalem artichoke tubers (Helianthus tu-berosus L.) M. Edelenbos Food Chem, 141(2), 1445–1450. doi: 10.1016/j.foodchem.2013.04.028.

Bach, V., Kidmose, U., Kjeldsen, G.B., & Edelenbos, M. (2012). Effects of harvest time and variety on sensory quality and chemical composition of Jerusalem arti-choke (Helianthus tuberosus L.) tubers Food Chem, 133(1), 82–89. doi: 10.1016/j.foodchem.2011.12.075.

Bezusov, A.T., Pylypenko, I.V., & Srednytska, Z.Iu. (2009). Vyvchennia fermentatyvnykh system top-inamburu dlia otrymannia inulinu ta inulinopodibnykh rechovyn in vitro. Naukovi pratsi ONAKhT, 36(2), 34–37 (in Ukrainian).

Bezusov, A.T., Verkhivker, Ya.H., & Storozhuk, V.M. (2017). Tekhnolohiia konservovanykh produktiv. lab. Praktykum. navch. posib. ta in. Odesa. Osvita Ukrainy (in Ukrainian).

Bilenka, I., Golinskaya, Y., Dzyuba, N., & Martirosian, H. (2018). Efect of pre-treatment on Qualitative indices of weite Roots. Food Science and Technology, 12(1), 34–42. doi: 10.15673/fst.v12i1.838.

Bilenka, I.R., & Lazarenko, N.A. (2015). Ozdorovchi produkty na osnovi topinambura: monohrafiia. Odes. nats. akad. kharch. tekhnolohii. Odesa (in Ukrainian).

Cherevko, O.I., Dudenko, N.V., Horban, V.H., Pavlotska, L.F., & Zhohlo, V.I. (2007). Sposib zakhystu ochyshchenykh bulb topinambura vid potemninnia: pat. na korysnu model 19897 Ukraina: MPK A 23 V 7/ 005/– № 200602841; zaiav. 13.03.2006; opubl. 15.01.2007, Biul. № 1 (in Ukrainian).

Gedrovica, I., Karklina, D., Fras, A., Jablonka, O., & Boros, D. (2011). The non–starch polysaccharides quantity changes in pastry products where Jerusalem artichoke (Helianthus tuberosus L.) added. Procedia Food Sci, 1, 1638–1644. doi: 10.1016/j.profoo.2011.09.242.

Kvitajlo, I.V. (2011). Razrabotka tehnologii ohlazhdennyh i zamorozhennyh kombinirovannyh salatov funkcional'nogo naznachenija. dis. kand. tehn. nauk: 05.18.01. Krasnodar (in Russian).

Pavlyuk, R., Bessarab, O., Pogarskaya, V., Balalai, K., & Loseva, S. (2015). Development of cryogenic tech-nology for the production of nano-powders from top-inambour using liquid and gaseous nitrogen. Eastern-European journal of enterprise technologie, 6/10(78), 4–10. doi: 10.15587/1729-4061.2015.56170.

Polygalina, G.V., Cherednichenko, V.S., & Rimareva, L.V. (2003). Opredelenie aktivnosti fermentov: spravochnik. M. DeLi print (in Russian).

Radovanovic, A., Stojceska, V., Plunkett, A., Jankovic, S., Milovanovic, D., & Cupara, S., (2015). The use of dry Jerusalem artichoke as a functional nutrient in de-veloping extruded food with low glycaemic index. A. Radovanovic. Food Chem, 177(15), 81–88. doi: 10.1016/j.foodchem.2014.12. 096.

Takeuchi, J., & Nagashima, T. (2011). Preparation of dried chips from Jerusalem artichoke (Helianthus tuberosus) tubers and analysis of their functional properties. Food Chem, 126(1), 922–926. doi: 10.1016/j.foodchem.2010.11.080.

Tchone, M. (2003). Über Polyphenole in Topinambur (Helianthus tuberosus L.) und andere gesundheitsrelevante Inhaltsstoffe. Doctoral Thesis. Tchone Michel. Berlin, 281 doi: 10.14279/depositonce-670.

Tchoné, M., Bärwald, G., Annemüller, G., & Fleischer, L.G. (2006). Separation and identification of phenolic compounds in Jerusalem artichoke (Helianthus tu-berosus L.) Sci. Aliments, 26, 394–408. doi: 10.3166/sda.26.394-408.

Telezhenko, L.N., & Bezusov, A.T. (2004). Biologicheski aktivne veshhestva fruktov i ovoshhej i ih soranenie pri pererabotke. Odessa. Optimus (in Russian).

Zawistowski, J., Blank, G., Murray, E.D. (1986). Poly-phenol Oxidase Activity in Jerusalem Artichokes. (Helianthus tuberosus L.) Food Res. Int., 19, 210–214. doi: 10.1016/S0315-5463(86)71669-6.

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Published
2019-04-23
How to Cite
Bilenka, I., Lazarenko, N., Zolovska, O., & Golinskaya, Y. (2019). Features of the enzyme composition of Jerusalem artichoke tubers. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Food Technologies, 21(91), 14-19. https://doi.org/10.32718/nvlvet-f9103