Determination of complex forming ability of mixed-ligand organic systems relative to the metal ions
It is shown that classical and specific methods for determining the complex forming ability of mixed-ligand organic systems relative to the metal ions is not perfect. Determination of complex-forming ability of mixed-ligand organic systems relative to the metal ions using the method of turbidimetry for media containing biometal chlorides, mixed-ligand systems and sodium carbonate is proposed. As mixed-ligand systems used the culture fluid Bifidobacterium bifidum AC-1670 (mixed-ligand system I), the culture fluid of the composition of probiotic bacteria (mixed-ligand system II), the culture fluid of the composition of probiotic bacteria with the introduction of exogenous chelating agents (mixed-ligand system III), culture fluid of probiotic bacteria composition and products of their cell walls processing (mixed-ligand system IV). A solution of metal chloride (magnesium or calcium, or cuprum, or manganese, or ferrum or zinc) was added discretely to the aliquots of the solution of the organic mixed-ligand system. The mixture was stirred and kept for 30 minutes at 40 °C, then a solution of sodium carbonate was added to the aliquots and discretely measured turbidity of the solution by turbidimetric method at a wavelength of 450 nm. When the increasing of the turbidity magnitude system by 0.02 opt. un, a conclusion about the maximum value of the mixed-ligand organic systems complex forming capacity relative to the metal ion was made. Further increase in the turbidity of the system indicates an increase in the metal content in inorganic form, respectively the complex formation potential of the mixed-ligand system is exhausted. It is determined that the highest complex forming ability in relation to ions of biometals has mixed-ligand system III. The proposed method allows precisely to determine the complex formation capacity of mixed-ligand organic systems in relation to metal ions without the use of aggressive reagents capable of destroying chelate bonds and without involving high-cost rare equipment.
Kapustian, A., Antipina, O., & Budiak, R. (2018). Obtain-ing and characteristic of the magnesium organic forms on the basis of products of bifidobacteria processing and their metabolites. Food Science and Technology, 12(3), 4–12. doi: 10.15673/fst.v12i3.1054.
Kapustian, A., Cherno, N., & Nikulina, О. (2018). Ob-taining and characteristics of calcium organic forms on the basis of metabolites and processing products of probiotic bacteria. Food Science and Technology, 12(2), 4–12. doi: 10.15673/fst.v12i2.944
Karkishchenko, N.N., Karkishchenko, V.N., Lyublinskiy, S.L., Kapanadze, G.D., Shustov, E.B., Revyakin, A.O. (2013). Rol mikroelementov v sportivnom pitanii i be-zopasnost metallokhelatov. Biomeditsina, 2, 12–41 (in Russian).
Klenin, V.I., Shchegolev, S.Yu., & Lavrushin, V.I. (1977). Kharakteristicheskiye funktsii svetorasseyaniya dispersnykh sistem. Saratov: Izd-vo Sarat. un-ta (in Russian).
Kornev, V.I., & Keppel, N.V. (2009). Smeshanoligand-nyye kompleksy medi (ІІ) s nitrilotriuksusnoy i limonnoy kislotami v vodnom ras tvore. Vestnik ud-murtskogo universiteta, 2, 25–31 (in Russian).
Mahmoud, N.A.Al-Jibouri, Salam, A.H.Al-Ameri, Wessal, M.Al-Jibouri, & Mohammed, A.K.Al-Souz. (2013). Spectroscopic study of the effect of a new metal chelate on the stability of PVC. Journal of the Association of Arab Universities for Basic and Applied Sciences, 14, 67–74. doi: 10.1016/j.jaubas.2012.11.003.
Nikolayeva, L.S., Semenov, A.N., & Burova, L.I. (2011). Smeshanoligandnoye kompleksoobrazovaniye ionov kaltsiya i magniya s geparinom i glitsinom. Zhurnal neorganicheskoy khimii, 56(4), 689–696 (in Russian).
Rabindra, P., & Mohan, A. (2000). Synthesis and charac-terization of mixed ligand complexes of bio-metals with pyrimidine nucleoside (uridine) and amino acids. Proc. Indian Acad. Sci. (Chem. Sci.), 112(6), 593–600. doi: 10.1007/BF02704366.
Rusu, D., Stanila, A., MarianI, O., & Marian, C.O. (2009). Synthesis and Characterization of Some Cobalt (II) Complexes with Amino Acids Having Biological Activities. Rev. Chim., 60(9), 939–943.
Shcheglova, N.V., Pechnikova, A.S., & Shevchenko, A.I. (2014). Smeshannoligandnyye kompleksy kobalta (ІІІ) s etilendiaminom i etilendiamintetrauksusnoy kislotoy v vodnykh rastvorakh. Vestnik Kazanskogo tekhnologicheskogo universiteta, 17, 56–59 (in Rus-sian).
Shcheglova, N.V., Pechnikova, A.S., Shevchenko, A.I., Smotrina, T.V., Popova, T.V., & Shkodich, V.F. (2014). Smeshannoligandnyye kompleksy kobalta (III) s etilendiaminom i etilendiamintetrauksusnoy kislotoy v vodnykh rastvorakh. Vestnik Kazanskogo tekhnologicheskogo universiteta, 17, 56–59 (in Rus-sian).
Siggia, S., Eichlin, D.W., & Rheinhart, R.C. (1955). Po-tentiometric Titrations Involving Chelating Agents, Metal Ions, and Metal Chelates. Anal. Chem., 27(11), 1745–1749. doi:10.1021/ac60107a019.
Tripathi, I.P., & Kamal, Aarti. (2015). Synthesis, Charac-terization of Some Complexes of Copper (II) with L-Asparginine, L-Histidine, L-Lysine. American Journal of Advanced Drug Delivery, 3(1), 95–103.
Verma, Sh., Singh, D., Kumar, R., Shukla, B.K., & Krishna, V. (2015). Equilibrium study and Stability constants of mixed Ligand complexes of Biomole-cules and Amino acids with Metal ions by Potentiom-etric method. Journal of Chemical Sciences, 5(3), 42–48. http://www.isca.in/rjcs/Archives/v5/i3/9.ISCA-RJCS-2015-032.php.
Abstract views: 1 PDF Downloads: 0