Etiological structure of bacteriosis of animals in the Dnipropetrovsk region for 2014–2016

Dnipropetrovsk Regional State Laboratory of Veterinary Medicine, Oleksandra Polya Ave., 48, Dnipro, 49000, Ukraine. Glebenyuk, V.V., Borovik, I.V., Kuchuk, T.V., & Litvinenko, O.O. (2018). Etiological structure of bacteriosis of animals in the Dnipropetrovsk region for 2014–2016. Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnologies. 20(83), 260–263. doi: 10.15421/nvlvet8351

The epizootic situation with regard to some infectious diseases in the Dnipropetrovsk region remains as complicated and strained.Timely and accurate diagnosis determines the effectiveness of the realized preventive measures of infectious diseases and establishes the peculiarities and changes of the epizootic situation in certain areas.The laboratory examinations, including the bacteriological method, have the crucial importance for the correct assessment of prevention and antiepizootic measures.Objective of this review: was to determine the etiological structure of infectious diseases in the Dnipropetrovsk region for 2014-2016, based on the results of bacteriological examinations.As the material for the examinations were the data of the veterinary reports of the State district veterinary medicine laboratories of the Dnipropetrovsk region and the results of our own examinations conducted on the basis of the Dnipropetrovsk Regional State Laboratory of the Veterinary Medicine (DRSLVM).As of 2014 year, in the Dnipropetrovsk region, the livestock capita in all categories of farms was: horned cattle -130.527, small cattle -44.739, pigs -520.801, horses -1.781, poultries -15.342,087, including chickens -14.816.157, waterfowl -525.930. During 2014cattle -130.527, small cattle -44.739, pigs -520.801, horses -1.781, poultries -15.342,087, including chickens -14.816.157, waterfowl -525.930. During -2016 by means of laboratories of veterinary medicine in the Dnipropetrovsk region have been conducted 18.474 examinations on the infectious diseases of the animals.142 positive results were received in total.According to the results of the bacteriological examinations in the Dnipropetrovsk region, for the years 2014-2016 were registered diplococcosis, malignant edema, pasteurellosis, colibacteriosis, salmonellosis, pullorosis, staphylococcosis, streptococcosis andswine erysipelas, poultry pseudomonosis, aeromonosis of fish, parafoul brood (caused by Bacillus paraalvei) and proteose of bees.4.472 examinations were conducted on colibacteriosis and 78 positive results were obtained.21 cultures were obtained from the pigs, from poultries -44 cultures, from embryos -6 cultures, from small cattle -3 cultures, from fur animals -2 cultures, from dogs -2 cultures.Cultures of Escherichia coli, isolated from pigs are represented by the serological variants: O139, O26, O9, O78, O1, O8 and atypical variants, and from poultries -O115, O119, O78, O15, O86, K88 AB and atypical variants. For salmonella,7.578

Introduction
The modern path of development of the Ukraine causes appearance of complex requirements and tasks in the field of animal husbandry, directed on the provision of the population with high-quality agricultural production in accordance with international standards of quality and security.Such production of animal origin should not contain pathogenic microorganisms and can only be obtained from healthy animals (Khomenko et al., 1995).
The epizootic situation with regard to some infectious diseases in the Dnipropetrovsk region remains as complicated and strained (Tkachenko et al., 2007;Ukhovs'kij et al., 2014;Bіla et al., 2014;Hlebenjuk, 2014;Hlebenjuk and Telіzhenko, 2015;Borovyk, 2016).Timely and accurate diagnosis determines the effectiveness of the realized preventive measures of infectious diseases and establishes the peculiarities and changes of the epizootic situation in certain areas (Konopatkin et al., 1984;Yarchuk et al., 2002).
The laboratory examinations, including the bacteriological method, have the crucial importance for the correct assessment of prevention and antiepizootic measures (Vasylieva, 2016;Ushkalov, 2017).
Objective of this review: was to determine the etiological structure of infectious diseases in the Dnipropetrovsk region for 2014-2016, based on the results of bacteriological examinations.

Materials and methods
As the material for the examinations were the data of the veterinary reports of the State district veterinary medicine laboratories of the Dnipropetrovsk region and the results of our own examinations conducted on the basis of the Dnipropetrovsk Regional State Laboratory of the Veterinary Medicine (DRSLVM).

Results and discussion
As of 2014 year, in the Dnipropetrovsk region, the live-stock capita in all categories of farms was: horned cattle -130.527,small cattle -44.739, pigs -520.801, horses -1.781, poultries -15.342.087, including chickens -14.816.157, waterfowl -525.930.As can be seen from the table, 71 samples were examined on swine erysipelas, including 5 samples from wild fauna.The positive result was found in 5 animals from Kryvorizkyi and Petrykivskyi districts.
From the 745 examinations on pasteurellosis, the diagnosis was confirmed in 3 animals (2 rabbits from the city of Dnipro and 1 lamb from Pavlogradskyi district).
By means of bacteriological examinations were determined the etiological significance of 12 staphylococcal cultures in the occurrence of inflammatory processes (vaginitis, conjunctivitis, dermatitis, etc.).Diplococcosis was recorded in 1 animal (Magdalinіvs'kij district).
Streptococcosis of pigs has been confirmed by 4 animals (Sinel'nikіvs'kij district).For malignant edema of swines, 1 examination was conducted and the positive result was obtained (Pyatyhatskyi district).
On pseudomonosis of the poultries, 73 examinatios were conducted and 6 positive results were obtained (Dniprovskyi and Pyatyhatskyi districts).In addition, the culture of Pseudomonas aeruginosa was isolated from the dog and lamb biomaterial.
From the 360 examinations on fish aeromonosis, the diagnosis was confirmed in 5 samples (Novomoskovs'kij and Dniprovskyi districts).
Among the diseases of bees, laboratory tests have been recorded parafoul brood and proteose of bees.

Conclusion
Causative agents of 13 infectious animal diseases have been identified in the Dnipropetrovsk region by means of bacteriological investigations.Most commonly isolated pathogens of intestinal infections (colibacteriosis and salmonellosis).

Fig.
Fig. Number of the bacteriological examinations on bacteriosis of the animals in the Dnipropetrovsk region for 2014-2016 (data, received from DRSLVM) According to the results of the bacteriological examinations in the Dnipropetrovsk region, for the years 2014-2016 were registered diplococcosis, malignant edema, pasteurellosis, colibacteriosis, salmonellosis, pullorosis, staphylococcosis, streptococcosis and swine erysipelas, poultry pseudomonosis, aeromonosis of fish, parafoul brood (caused by Bacillus paraalvei) and proteose of bees.