Toxicokinetics metal nanoparticles in day-old chicks provided administration laying hens with feed nanocomposite metals (Ag, Fe, Cu and Mn dioxide)


  • A.L. Orobchenko National Scientific Center «Institute of Experimental and Clinical Veterinary Medicine»
  • A.T. Kutsan National Scientific Center «Institute of Experimental and Clinical Veterinary Medicine»
  • A.A. Shmatko National Aerospace University «Kharkiv Aviation Institute»
Keywords: toxicokinetics, nanocomposite metal dose, laying hens, day-old chicks, scanning electron microscopy

Abstract

The article presents data on the toxicokinetics of nanoparticles (NP) of metals in the body day-old chicks in the conditions of introduction of laying hens with feed nanocomposite (Ag, Cu, Fe, Mn dioxide). The sample of nanocomposite comprises metal nanoparticles (NрMe) of silver (31.5 ± 0.9 nm), iron (100.0 ± 10.0 nm), copper (70.0 ± 4.0 nm) and manganese dioxide (50.0 ± 3.0 nm) in relation to aliquot at a final concentration of 100 mg/sm3 for each metal. The experiment was conducted on roosters and laying hens breed Hajseks White. By the principle of analogues 3 groups were formed of birds with a sex ratio of roosters to hens 1:6. Laying hens in the control group was further introduced the saline into feed, birds of experimental groups for 37 days had been administered daily feed additive: the first group – in the biotic NрMe dose (0.3 mg/kg), II – NрMe in toxic dose (4.0 mg/kg). Starting from the 30th day of the birds in each group during the week laid eggs were collected and laid them on the incubation. After hatching performed euthanasia and selection of organs and tissues: yolk sac, muscular stomach, gastrointestinal tract, liver, lungs, heart, brain, muscles, bones and skin with a pen. Determination of nanoparticles and corresponding metal identification performed by the scanning electron microscope. It is found that the metal nanoparticles distributed in organs and tissues of chickens with the highest concentration in liver at a dose 4.0 mg/kg body weight (max iron 139.71 nр/kg, copper 71.78 and manganese dioxide 13.21 nр/kg ) and heart (max iron 217.07 nр/kg, copper 100.89 and manganese dioxide 3.84 nр/kg). Thus, regardless of the dose NP almost identically (except manganese) used with the yolk sac. A large concentration of NP has been identified in the brain and lungs day-old chicks, which may indicate a tropism NP to these tissues. It is possible that the accumulation of iron NP, copper, and manganese dioxide in the bones with muscles and skin with a pen (dose of 4.0 mg/kg body weight) resulted in violations of the musculoskeletal system, installed by us in previous studies.

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Published
2017-02-10
How to Cite
Orobchenko, A., Kutsan, A., & Shmatko, A. (2017). Toxicokinetics metal nanoparticles in day-old chicks provided administration laying hens with feed nanocomposite metals (Ag, Fe, Cu and Mn dioxide). Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 19(73), 19-24. https://doi.org/10.15421/nvlvet7304