Biochemical criteria for evaluation of functional status of dogs bone marrow
AbstractBone marrow is a complex of cells, that support hematopoiesis in an organism of animal. The bone marrow cells (BMC) have high therapeutic potential, it gives an opportunity to use them for treatment of different destructions of hematopoiesis and after chemotherapy. Thus, a clinical requirement in marrow increases constantly and requires creation of pool of biomaterial. In clinical veterinary and transplantation there is a requirement to create long-term storage of bone marrow cells by freezing technology. Using cryoprotectant preserves cell structure close to the control, but research is conducted, to study the mechanisms and the fight against the negative effects of cryodestruction. Despite the fact that these days the hematopoietic system cells are studied actively many aspects of their biochemistry are still uncertain and deserve special attention. The glucose-6-phosphate, ATP, pyruvic acid and lactic acid in the dogs` bone marrow cells before and after cryopreservation with dimethylsulfoxide have studied. It was found that 7% DMSO was the most effective cryoprotectant and survive 83.51 ± 1.9% of cells. The ATP, pyruvic and lactic acids levels in dogs` bone marrow cells after freezing-thawed with DMSO were near to the control measure.
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