Biochemical criteria for evaluation of functional status of dogs bone marrow


  • L.A. Vodopyanova
  • K.D. Yugay
  • O.N. Bobritskaya
  • S.L. Antipin
Keywords: bone marrow, blood, cryodestruction, cryopreservation, glucose-6-phosphate, adenosinetriphosphate, pyruvic acid, lactic acid

Abstract

Bone marrow is a complex of cells, that support hematopoiesis in an organism of animal. The bone marrow cells (BMC) have high therapeutic potential, it gives an opportunity to use them for treatment of different destructions of hematopoiesis and after chemotherapy. Thus, a clinical requirement in marrow increases constantly and requires creation of pool of biomaterial. In clinical veterinary and transplantation there is a requirement to create long-term storage of bone marrow cells by freezing technology. Using cryoprotectant preserves cell structure close to the control, but research is conducted, to study the mechanisms and the fight against the negative effects of cryodestruction. Despite the fact that these days the hematopoietic system cells are studied actively many aspects of their biochemistry are still uncertain and deserve special attention. The glucose-6-phosphate, ATP, pyruvic acid and lactic acid in the dogs` bone marrow cells before and after cryopreservation with dimethylsulfoxide have studied. It was found that 7% DMSO was the most effective cryoprotectant and survive 83.51 ± 1.9% of cells. The ATP, pyruvic and lactic acids levels in dogs` bone marrow cells after freezing-thawed with DMSO were near to the control measure.

Downloads

Download data is not yet available.

References

Warry, E.E., Willcox, J.L., Suter, S.E. (2014). Autologous peripheral blood hematopoietic cell transplantation in dogs with T-cell lymphoma. Vet. Intern. Med. 28(2), 529–37.

Вaust, J.G., Baust, J.M. (2007). Advances in biopreservation. Ed. by - By Tayloor & Francis Group. LLC, 15–62.

Mamprin, M.E., Vega, F., Rodrigues, J.V. (2005). Adenosine 5 triphoshate transport and accumulation during the cold preservation of rat hepatocytes in University of Wisconsin solution. World J. Gastroenterol. 11(13), 1957–1964.

Gajduk, M.B., Gutyj, B.V., Gufrij, D.F. (2016). Therapeutic effectiveness of the drug RBS – DOG as immune modulating means in the treatment of dogs with wounds at hypo ergic type of inflammation. Scientific Messenger LNUVMBT named after S.Z. Gzhytskyj, 18, 2(66), 35–39.

Goltsev, A.M., Dubrava, T.G., Kozlova, Yu.A., Gurina, T.M., Ostankov, M.V. (2005). Ocenka gemopojeticheskogo potenciala stvolovyh krovetvornyh kletok kostnogo mozga s izmenennym ishodnym statusom pod dejstviem faktorov kriokonservirovanija. Problemy kriobiologii. 15(3), 320–323 (in Russian).

Kawano, Y., Lee, C.L., Watanabe, T., Abe, T. (2004). Cryopreservation of mobilized blood stem cells at a higher cell concentration without the use of a programmed freezer. Ann. Hematol. 83(1), 50–54.

Cucaeva, A.A., Agranenko, V.A., Fedorova, L.I. (1983). Kriokonservirovanie kletochnyh suspenzij. Pod red. A.A. Cucaevoj. – K.: Nauk. dumka, 240 (in Russian).

Vodop'janova, L.A., Zhegunov, G.F. (2006). Sohrannost' kletok kostnogo mozga sobak posle jekspozicii s krioprotektorami i zamorazhivanija v zhidkom azote. Problemy kriobiologii. 16(2), 207-210 (in Russian).

Idei, K., Matsuura, S., Fujino, Y. (2008). Investigation of Various Methods for the Cryopreservation of Canine Bone Marrow- Derived CD34+ Cells. J. Vet. Med. Sci. 70(11), 1211–1217.

Prohorova, M.I., Zolotova, L.A., Flerov, M.A. (1982). (1982). Metody biohimicheskih issledovanij (lipidnyj i jenergeticheskij obmen). L.: Izd. Leningrad. univer. (in Russian).

Patel, S.D., Paroutsakis, E.T., Winter, J.N., Muller, W.M. (2000). The lactate issue revisited: novel feeding protocols to examine inhibition of cell proliferation and glucose metabolism in hematopoietic cell cultures. Biotechnol. Prog. 16(5), 885–892.

Abstract views: 127
PDF Downloads: 99
Published
2017-02-05
How to Cite
Vodopyanova, L., Yugay, K., Bobritskaya, O., & Antipin, S. (2017). Biochemical criteria for evaluation of functional status of dogs bone marrow. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 19(73), 37-39. https://doi.org/10.15421/nvlvet7308