Bactericidal and disinfective properties of disinfectant «Alkisept–100»


  • O.L. Tishyn
  • G.T. Kopijchuk
  • R.V. Khomiak
  • O.V. Khyrivskyy
  • M.M. Danko
Keywords: disinfectant «Alkisept–100», test-culture, bactericidal dilution, bactericidal concentration, phenol coefficient, protein index, test objects, disinfection

Abstract

The results of research bactericidal properties of new domestic disinfectant «Alkisept–100» that based on a glutaraldehyde, didecyldimethylammonium chloride, alkyldimethylbenzylammonium chloride and aminotrismethylenephosphonic acid. Was found bactericidal dilution (BD), bactericidal concentration (BC), phenol coefficient (FC) and protein index (PI). Most susceptible to the effect of disinfectant was test-cultures of P. vulgaris, where cell death came on the 10 and 30 minutes exposures at concentrations of 0.26 and 0.18%, respectively. The action of disinfection preparation for gram-positive bacteria S. aureus, vegetative cells of B. subtilis vegetative cells and cultures of S. typhimurium test-cell death drew under these conditions at concentrations of 0.72 and 0.52%, respectively. Gram-negative bacteria E. coli were more susceptible to disinfectant and cell death drew for these exposures at concentrations of 0.52 and 0.37 %, respectively. BD of this disinfectat were 1.96 and 1.4 higher times for E. coli and S. aureus, respectively, from BD phenol, and in the presence of protein activity investigated disinfection preparation is reduced to 2.74 times. The efficacy of disinfection preparation of microorganisms on surfaces in the decontamination of test objects. It is established that test cultures of E. coli and S. aureus (without organic impurities) at 0.1% concentration of disinfectant is ineffective. For spore form of B. subtilis disinfection preparation concentration at 4.0%. Is ineffective At the 1.0–1.5% and higher concentrations is effective for surface treatment of wood, metal and tile that are contaminated with organic substances and objects subject to veterinary controls, and for elimination spore forms of microorganisms at its working concentration of desinfectant should be 5.0% or higher, the exposure in 120 minutes or more. Proved that the disinfectant «Alkisept–100» in a production environment at a concentration of 1.0% by wet surface irrigation facilities for animals and working solution 0.25–0.3 liters per 1 square meter with an exposure of 2 hours, showing high disinfectant properties.

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PDF Downloads: 92
Published
2017-02-03
How to Cite
Tishyn, O., Kopijchuk, G., Khomiak, R., Khyrivskyy, O., & Danko, M. (2017). Bactericidal and disinfective properties of disinfectant «Alkisept–100». Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 19(73), 61-65. https://doi.org/10.15421/nvlvet7313