Determination of sensitivity production strains Bacillus anthracis UA–07 to antibiotics


  • I. Rublenko Bila Tserkva National Agrarian University, Soborna sq., 8/1, Bila Tserkva, Kievska obl., 09117
  • V. Skripnik The State Scientific Research Institute of Laboratory Diagnostic and Veterinary Sanitary Expertise, Donetska Str., 30, Kyiv, 03151
Keywords: anthrax, sensitivity, stability, resistance, growth zone, Bacillus anthracis UA–07 antibiotics concentration, paper disc treatment

Abstract

Long-term use of antibiotics leads to antibiotic resistance in pathogens. In addition, there is a possibility of permanent genetic changes in strains due to environmental influences. Therefore there is a need for periodic study vaccine strains for sensitivity to antibiotics in order to study and control of their passport characteristics. The aim of our research was necessary to determine the sensitivity of the strain of Bacillus anthracis UA-07 to antibiotics. The results of studies of sensitivity of strain Bacillus anthracis UA–07 to 50 antibiotics were determined by the size of the diameter of the zones of growth retardation. Zone growth delay of 15 mm was considered a sign of a weak antibiotic sensitivity, zone 15–24 mm – a sign of sensitivity and area of more than 24 mm - a sign of the high sensitivity of the strain to this antibiotic. Lack of stunted growth areas points to the insensitivity of the strain to this antibiotic. The research results show that the strain of Bacillus anthracis UA–07 is highly sensitive to ofloxacin (Of5), ampicillin / sulbactam (A/S10/10), linezolid (LZ30), amoxicillin (AMX30), norfloxacin (NX10), cefalotin (CEP30), tetracycline (TE30). Not affect the development of the studied microorganism antibiotics, cefepime (SPM30), nystatin (NS100U) tsefuroksyn (CXM30) tsefeksym (CFM5), sulfadiazine (SZ300), metronidazole (MT4), cefazolin (Cz30).

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Abstract views: 127
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Published
2017-02-04
How to Cite
Rublenko, I., & Skripnik, V. (2017). Determination of sensitivity production strains Bacillus anthracis UA–07 to antibiotics. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 19(73), 84-88. https://doi.org/10.15421/nvlvet7318