Reproductive capacity and structural and functional status rats uterus that consumed feed containing 20% GM-soy
The article presents the dynamics of changes in female fertility of I, II and III generations of rats and morph functional state of rats uterus of I generation, which were fed with feed containing 20% traditional and genetically modified soy. Received three generations of rats that consumed feed containing 20% of GM and traditional soybeans. It was received generation of rats that consumed feed containing 20% of GM and traditional soybeans. The reproductive ability was evaluated by the fertility of animals. On 135 day of life 5 rat females from each group (60 days after birth) were taken out of the experiment through ether anesthesia, were decapitated, the uterus were taken for histological and histochemical research, which were fixed in 10% solution of neutral formalin, Karnua liquid, followed by pouring into paraffin. It was produced histoslices, stained with hematoxylin-eosin, by McManus combined with altsianic blue.
It was established that long-term feeding of feed containing 20% GM soya negatively affected the 1 generation of animals, as expressed lowering of reproductive capacity of female rats and tendentious decrease in the number of heads of newborns in the young rat nests. In the next two generations of female rats of II and III groups probable differences in the investigated indices were not found which is probably due to the inclusion of adaptive compensatory mechanisms to change feeding ration.
For histological examination of the rats uterus of the I generation, who consumed feed containing 20% soybean was set to reduce the secretion of glycans replacement, which has reduced barrier protection, damage of epithelial cells, development of atrophic processes in the myometrium and endometrium, cyst like extension of one uterine glands and vacuolic epithelial dystrophy of others, lymphoid-histiocytic infiltration of the lamina propria of the endometrium, indicating that the decline in morphological functional state of the uterus.
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