Influence of probiotic and phytobiotic on parameters of T- and B-cell immunity in weaned piglets with non-contagious gastroenteritis

  • B.O. Lukashchuk Lviv National University of Veterinary Medicine and Biotechnologies
Keywords: weaned piglets, antibiotic, probiotic, phytobiotic, gastroenteritis, immune system, T-lymphocytes, T-total, T-active, T-helper, T-suppressor, B-lymphocytes, immunoregulatory index


In the use of colostral protective factors and insufficient activity of piglets immune system, against the background of immunodeficiency after weaning from sows developing diseases, often caused by conditionally-pathogenic or pathogenic microorganisms, including non-contagious gastroenteritis. The article presents analyzes of T- and B-cell immunity parameters in weaned piglets with non-contagious gastroenteritis and after their treatment with probiotic TOYOCERIN 10⁹ and phytobiotic EXTRACT™ 6930 as part of the basic diet in combination with antibiotic (10 % solution of enrofloxacin hydrochloride) in the modern pig farm. It was established that in piglets after weaning with non-contagious gastroenteritis decreased of T- and B-cell immunity parameters. In particular, in blood decreased number of total and active T-lymphocytes, T-helpers and T-suppressors also decreased number of B-lymphocytes, which may indicate the development in these immunodeficient condition. It was established positive effect of probiotic and phytobiotic in combination with antibiotic on T-cell immunity of pigs, as indicated by the increased number of total T-lymphocytes in blood to the clinical healthy animals level. A similar tendency was observed in the number of T-helper cells, their number was higher in groups of weaned piglets which added probiotic TOYOCERIN 10⁹ and phytobiotic EXTRACT™ 6930. As to the immunoregulatory index, which is a quantitative ratio of T-helper to T-suppressor, there was no significant alteration of values in control and experimental groups of pigs. It was established positive effect of probiotic and phytobiotic in humoral immunity of piglets by increasing number of B-lymphocytes to the level of clinical healthy animals which indicate increased body ability to the active synthesis of protective antibodies. The use of probiotic TOYOCERIN 10⁹ and phytobiotic EXTRACT™ 6930 for weaned piglets with non-contagious gastroenteritis normalized number of T- and B-lymphocytes, which are the leading immunocompetent blood cells that characterize the level of animal body defense and condition of specific immunity. Perspectives for further research will be directed at determining the therapeutic effectiveness of probiotic TOYOCERIN 10⁹ and phytobiotic EXTRACT™ 6930 for weaned piglets with non-contagious gastroenteritis.


Download data is not yet available.


Schubot, R.M., Tizard, I.R. (2004). Veterinary immunology: An Introduction. 7th ed. – Philadelphia.

Salyga, N. (2009). Rozvytok imunnoi' systemy u porosjat. Visnyk L'vivs'kogo universytetu. Serija biologichna. 51, 3–14 (in Ukrainian).

Bulter, J., Sinkora, M., Wertz, N. (2006). Development of the neonatal B and T cell repertoire in swine: implications for comparative and veterinary immunology. Vet. Res. 37, 417–441.

Sinkora, J., Rehakova, Z., Sinkora, M. (2002). Early development of immune system in pigs. Vet. Immunol. Immunopathol. 87, 301–306.

Karput', I.M., Babina, M.P., Nikoladze, M.G., Babina, T.V. (2005). Diagnostika i profilaktika vozrastnyh i priobretennyh immunnyh deficitov. Vescі nacyjanal'naj akadjemіі navuk Belarusі. Seryja agrarnih navuk. 1, 67–70 (in Russian).

Vondruskova, H., Slamova,R., Trckova, M. (2010). Alternatives to antibiotic growth promoters in prevention of diarrhoea in weaned piglets: a review. Veterinarni Medicina. 55(5), 199–224.

Vishchur, O.I., Gutyj, B.V., Hufrii, D.F. (2015). Imunnyi status, sposoby otsinky i metody korektsii u teliat rannoho viku: monohrafiia. Lviv: SPOLOM (in Ukrainian).

Kocjumbas, I.Ja., Gunchak, V.M., Stec'ko, T.I. (2013). Problemy vykorystannja antymikrobnyh preparativ dlja stymuljuvannja rostu produktyvnyh tvaryn ta al'ternatyvy i'h zastosuvannju. Naukovo-tehnichnyj bjuleten' Instytutu biologii' tvaryn i Derzhavnogo naukovo-doslidnogo kontrol'nogo instytutu vetpreparativ ta kormovyh dobavok. 14(3–4), 381–389 (in Ukrainian).

Kuz'menko, O.A., Chernjuk, S.V. (2012). Vplyv prebiotyka ta kormovogo antybiotyka na imunologichni pokaznyky krovi ta mikrobnyj status travnogo kanalu svynej. Zbirnyk naukovyh prac' VNAU. 4(62), 10–14 (in Ukrainian).

Todoriuk, V.B., Gutyj, B.V., Khomyk, R.I., Vasiv, R.O. (2016). Influence of ferrovet 7.5% and ferosel T on the concentration of mineral substances in the blood serum of piglets suffering from Iron deficit anemia. Scientific Messenger LNUVMBT named after S.Z. Gzhytskyj. 18, 3(71), 139–143.

Ariza-Nieto, C., Bandrick, M., Baidoo, S.K. (2011). Effect of dietary supplementation of oregano essential oils to sows on colostrum and milk composition, growth pattern and immune status of suckling pigs. Journal of Animal Science. 89, 1079–1089.

Yuan, S.L., Piao, X.S., Li, D.F. (2006). Effects of dietary Astragalus polysaccharide on growth performance and immune function in weaned pigs. Animal Science. 62, 501–507.

Li, Z.J., Yi, G.F., Yin, J.D. (2008). Effects of organic acids on growth performance, gastrointestinal pH, intestinal microbial populations and immune responses of weaned pigs. Asian-Australasian Journal of Animal Sciences. 21, 252–261.

Heo, J.M., Opapeju, F.O., Pluske, J.R., Kim, J.C. (2012). Gastrointestinal health and function in weaned pigs: a review of feeding strategies to control post-weaning diarrhoea without using in-feed antimicrobial compounds. Journal of Animal Physiology and Animal Nutrition. 97, 207–237.

Levchenko, V.I., Zajarnjuk, V.P., Papchenko, I.V. (2005). Hvoroby svynej: pidruchnyk. Bila Cerkva (in Ukrainian).

Koval'chuk, Ja.Ja., Vishhur, O.I., Vlizlo, V.V. (2009). T- i V-limfocyty krovi porosjat pry vidluchenni i'h vid svynomatok ta za umov zgodovuvannja biomasy drizhdzhiv Saccharomices cerevisia. Biologija tvaryn. 11(1/2), 287–292 (in Ukrainian).

Abstract views: 113
PDF Downloads: 74
How to Cite
Lukashchuk, B. (2017). Influence of probiotic and phytobiotic on parameters of T- and B-cell immunity in weaned piglets with non-contagious gastroenteritis. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 19(73), 173-177.