The dynamics of leukocytes in piglets' blood at different stress periods of ontogenesis while feeding with feed additives «B-glukan» and «Biovir»
AbstractThe article provides the results of the research of the number of different types of leukocytes in blood of piglets at stress periods of ontogenesis while adding to their ration probiotic feed additives «B-Glukan» and «Biovir». There was formed three groups for conducting the research – Control (K), and two research (D1, D2), with 10 piglets in each group. Starting from 5th to 45th day age, piglets of the D1 group were fed with feed additive «B-Glukan», piglets of D2 group were fed with feed additive «Biovir» with the amount of 10 mg/kg of body mass per day. At the 28th day piglets were separated from sow. The material for the research was blood, which was taken in the morning, before the process of animals feeding, using the method of puncture of cranial vena cava at the 25th day of life (period before separation), at the 29th day of life (1 day after separation), at the 35th day of life (7 days after separation), at the 58th day of life (20 days after separation), at the 88th day of life (60 days after separation). The blood was determined by the absolute content of granulocytes, lymphocytes and monocytes using hematology analyzer MITIKH-18 (Switzerland). It is proven that while adding for D1 and D2 groups of piglets feed additives «B-Glukan» and «Biovir» in amount of 10 gr/kg body mass per day during 5th–45th day age promotes oxygen-transport function of blood, normalization of physiological state of an animals' organism in the period of adaptation to the stress action due to the process of separation, that can show the increase of leukocytes content in blood in 1.9 and 2.6 times (Р < 0.01), owing to granulocytes, lymphocytes and monocytes, in comparison with the animals of K group. Better result at the analysis of experimental material was obtained in piglets of D2 group, which were fed with feed additive «Biovir».
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