Activity and isozyme content of aspartate transaminase in cow reproductive organs
AbstractActivity and isozyme content of aspartate transaminase in cow reproductive organs at different physiological states and ovarian hypofunction were studied. It was registered, that activity of aspartate transaminase (AST) in ovarian tissue and endometrium at corpus luteum state is 132.0 ± 8.23 and 172.9 ± 8.70 μm/min×mg of protein, lower on 26.3 and 5.5% at follicle growth and at early corpus luteum is 66.5 ± 2.10 and 147.1 ± 13.26 μm/min×mg of protein. Lowest AST activity is registered at hypofunction state (61.8 ± 4.57 і 146.1 ± 9.33 μm/min×mg of protein). In cow reproductive organ tissues and antral fluid from ovarian follicles was registered two bands of proteins, that have AST activity. In reproductive organs main part is mitochondrial isozyme (АSТ2; 78.8–97.7%) in antral fluid – cytosole (АSТ1; 72.8–88.2%). In ovarian and uterus tissues was established high enzyme activity at late corpus luteum, that characterizes intensive aspartate conversion to oxaloacetate and possible utilization of substeates to maintain energetic and sytnthetic needs in cow reproductive organs. Antral fluid of big follicles from ovarian at follicle growth and at state of late corpus luteum characterizes by high activity and content of AST, that points on follicle cell growth – granulose and oocyte. In ovarian tissue and endometrium at hypofunction lower activity and isozyme content point on substrate deficiency for transamination and violation of physiological functions.
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