Protein and amino acid metabolism in the muscles of broiler chickens cross COBB 500 during treatment feed additive «Humilid»


  • E.O. Myhaylenko
  • O.O. Dyomshyna
  • L.M. Stepchenko
Keywords: broiler chickens, bioactive feed additive «Humilid», ALT, AST, LDH, GTP, ALP, protein metabolism

Abstract

The article presents data on the study of the impact of feed additives «Humilid» indicators on protein and amino acid metabolism of muscles of broiler chickens cross the COBB 500.The study tested that birds which additived Humilid the water increase in the muscles of total protein, which represented the largest share of the cytosolic and mitochondrial fractions. In homogenate of muscle, the total amount of protein increased by 10% in cytosolic and 20% in mitochondrial, which makes it possible to assert that stimulate the synthesis of cytosolic proteins is influenced Humilid and stimulated the formation chondriome of myocytes. Also, the data indicate an intensification of the use of amino acids for protein biosynthesis and adaptive processes, confirmed by increased in muscle mitochondrial fraction 2 times activity of gamma-glutamyltranspeptidase, which is involved in the transport of amino acids and glutathione in mitochondria that seen as a protective mechanism. The research has shown increased 3 times in cytosolic fraction activity of alanine aminotransferase and the simultaneous decrease in lactate dehydrogenase. Calculate the ratio activity of LDH/ALT showed bias towards anaerobic conversion of glucose to glucose-alanine cycle, more efficient way of recovery and using of glucose.

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Published
2017-03-12
How to Cite
Myhaylenko, E., Dyomshyna, O., & Stepchenko, L. (2017). Protein and amino acid metabolism in the muscles of broiler chickens cross COBB 500 during treatment feed additive «Humilid». Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 19(77), 110-116. https://doi.org/10.15421/nvlvet7725