The use of molecular biological research methods in applied fields of veterinary science
AbstractThe article deals with the information on the use of small amounts of molecular research methods in applied fields of veterinary science. Is also considered the main approaches and directions in the sector work of molecular biological research GNIKI of veterinary medicines and feed additives. The objects of researches of molecular biology are specific macromolecule – especially proteins and nucleic acids, the simplest form of living – isolated cell nucleus, organelles of cells, chromosomes, viruses and bacteria. The use of gene technology to analyze food quality for people and feeding stuffs for animal is due to the need for sensitive, rapid and accurate method. Using the PCR method is common for indication and identification of the causative agents of food poisoning. Based on the PCR it was developed methods for analyzing the products, which were made for the use of transgenic microorganisms. The actual use of the PCR is the determination of clinical strains antibiotic resistance of infectious diseases, creating the possibility of quantifying results account to control the dynamics infectious process, the correct choice of treatment and evaluating the effectiveness of used medicines. An important direction of the application of DNA technology is to identify products obtained after using genetic engineering techniques. Thereby, by means of molecular biological research methods the sector of molecular biological studies can perform the following tasks: detection, identification and quantification of the content of genetically modified organisms; identification of plant species of animal protein in premixes, fodder additives and animal feed; conducting monitoring investigations the content of genetically modified organisms and ruminant DNA in premixes, feed additives and animal nutrition according to an annual monitoring plan of feed, feed additives and premixes; in the future diagnosing infectious (bacteria) and parasitic exciter in farm animals and determination of antibiotic resistance in clinical strains of infectious diseases.
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PCR technology: principle and applications for DNA amplification; ed. H.Erlich. New York: W.H. Freeman and Company,1992.
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