Oxidative processes intensity and quallity of bull semen when adding microelements nanosuccinate compounds
AbstractWe investigated influence of organic compounds (succinates) of Mn2+, Сu2+ and Zn2+ on the intensity of oxidative processes in sperm and the survival of spermatozoa in bull ejaculates diluted by lactose-yolk-glycerin diluent. Respiration activity in control is 2.7 ± 0.44 ng-atom О/min×0,1 ml of semen, and when adding microelements succinate compounds changes and depends on dose and role of microelement in spermatozoa metabolism. When adding 0,06 mg/l Zn2+-succinate, respiration activity is on control level. Dose increase to 0.6 mg/l leads to decrease oxygen consumption on 44.5%, and at highest dose – 3.0 mg/l respiration activity is 1.2 ± 0.33 ng-atom О/min×0,1 ml of semen. Impact strength of Zn2+-succinate increasing content in diluted bull sperm on respiration activity is medium (η = 0.529). Analogically, when adding 0.004 mg/l Сu2+- and 0.01 Mn2+-succinate respiratory activity is on control levels: respiration intensity at maximal doses is lower on 59.3 and 66.7% (Р < 0.01–0.001). Impact strength of Сu2+ and Mn2+-succinate increasing concentration in diluted bull sperm on respiration activity is medium (η = 0.544 and 0.600). Simultaneously, addition of increasing doses of microelements succinates decreases redox ability on 0.05–0.11 mV/min×0.1 ml of semen. Thus, the number of sperm samples which revealed enhancing the potential of environment increases. Correlation between redox ability and increase of microelement succinates in diluted semen is η = 0.544–0.831. When adding Zn2+-succinate in dose 0,06 mg/l succinate dehydrogenase activity has a decreasing tendensy (on 16.6%; 44.2 ± 8.03 UI/h×0.1 ml of semen), and higher conсentrations lowers on 9.8–20.4% (Р > 0.05) comparing to control. Analogical changes are registered when adding Mn2+-succinate, but at maximal dose (1.0 mg/l) decrease is higher (30.3% comparing to control (Р < 0.05). Сu2+-succinate has a negative effect on succinate dehydrogenase activity – independently from dose activity is lower than in control and is: at 0.04 mg/l – on 27.3% and at 0.4 mg/l – on 58.3% (P < 0.05). When adding low doses of succinate microelements to diluent, spermatozoa survival is on control level (152.0–168.0 hours). Addition of 0.1 mg/l Мn2+-succinates increases spermatozoa 12 hours to 172.0 hours, and at maximal doses of Zn2+- і Сu2-succinate survival lowers on 12 hours comparing to control.
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