Formation mechanisms of immune cells protection in pigs under the influence of «Imunolak»


  • A. Kokarev Dnipropetrovsk state agrarian-economic university
  • D. Masiuk Dnipropetrovsk state agrarian-economic university
Keywords: leukocytes, leucogram, lymphocytes, resistance, newborn piglets, T-helper and T-suppressor, B lymphocytes, NK-lymphocytes, 0-lymphocytes, imunolak

Abstract

The article presents data about the features of cellular mechanisms of immune protection in neonatal pigs for drug action «Imunolak». The aim was to determine features of the formation mechanisms of cellular immune defense sucking pigs by the action of the drug «Imunolak» in the system «mother-fetus-newborn». This work was done in the SIC of biosafety and environmental control of agriculture resources of Dnipropetrovsk state agrarian-economic university and in the PrAT «Agro-Soyuz» («Agro-Soyuz», JSC) Synel’nykovskoe district of Dnepropetrovsk region. For the experiment were formed research and control groups of sows hybrids of white and Landrace breeds on the pair’s analogues principle. Each group consisted of 10 animals. Sows of research group were injected preparation «Imunolak» at 60, 75 and 90 days of gestation (TU 21.2-00493675-006:2013) at a dose of 0.05 mg of active ingredient per 1 kg of body weight, and the animals of the control group were injected 0.9% solution of NaCl. Blood samples were taken from these pigs are was derived from these sows before and 4 hours after ingestion of colostrum and 3, 7, 14 and 23 days of life. The number of leukocytes in whole blood were determined by an automatic hematology analyzer «PCE-90 VET», made in USA. Value of different forms of leukocytes determined in blood smears stained with Romanovsky-Giemsa. Determination of T lymphocytes, including T-helper and T-suppressor, conducted in the reaction of spontaneous rosette formation with sheep erythrocytes according to Jondal. et al (1972). Number of B-lymphocytes and NK-lymphocytes was determined in the reaction of spontaneous rosette formation of erythrocytes on the surface are adsorbed monoclonal antibodies against cellular receptors of lymphocytes CD 22 (B-cells) and CD 16 (NK-cells). Level 0-lymphocytes was determined by subtracting the total amount of lymphocytes T, B and NK-lymphocytes. The results characterize the physiological processes of formation of cellular mechanisms of immune protection in pigs of the early postnatal period and the effectiveness of their drug correction Immunotropic «Imunolak» in the system «mother-fetus-newborn». It is established that the use of sow gestating in the second half of the drug «Imunolak» promotes physiological increase in the number of leukocytes in furthering the first week of life, on average, 45.8%. This is due to the increase in the percentage of lymphocytes by 23.7%. Change is the result of recent increase in the absolute number of T lymphocytes, B lymphocytes and NK-lymphocytes average respectively 2.06, 1.98 and 2.31 times, and the relative decrease in the number of neutrophils 17.5%.

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Published
2017-03-06
How to Cite
Kokarev, A., & Masiuk, D. (2017). Formation mechanisms of immune cells protection in pigs under the influence of «Imunolak». Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 19(77), 214-219. Retrieved from https://nvlvet.com.ua/index.php/journal/article/view/1211