The impact of cell cultures transplantation on the course of experimental diabetes mellitus in animals
As we know from the literature data, type 1 diabetes mellitus occurs due to the loss of β-cells of island of Langerhans, manufacturing insulin, by the organism, which causes its deficiency. The treatment of this type of diabetes, even at early stages, lies in the substitution therapy together with a careful control of blood glucose which may last for life. It is for this reason that the cellular technologies as an alternative method of treatment of this pathology are growing more and more relevant.
Objective of the study: to study the impact of allogenic transplantation of the cultures of bone marrow, lipid tissue and pancreas cells on the course of experimentally generated diabetes mellitus in animals.
Task: 1. To obtain the cultures of bone marrow, lipid tissue and pancreas cells in rats. 2. To compare the impact of allogenic transplantation of the cultures of bone marrow, lipid tissue and pancreas cells in the course of experimentally generated diabetes mellitus in rats. 3. To study the impact of pancreas cells culture on the course of experimental diabetes mellitus in cats.
The studies were conducted using clinically healthy animals (30 males of white non-pedigree rats with body weight of 200–250 g, aged 4–5 months; 9 white non-pedigree junior rats aged 12 days; 4 mongrel cats aged 15–17 months) and missed fetuses of cats remained after obstetric aid.
Alloxan diabetes was generated by means of single subcutaneous injection of alloxane monohydrate in the dose of 150 mg/kg in the form of 5% solution on citrate buffer (pH 4.5) after preliminary 24-hour absolute diet with free access to water.
The cultures of bone marrow and pancreas cells were obtained from the bone marrow of tubular bones and pancreas of puppies aged 12 days correspondingly, lipid tissue – from rats aged 4–5 months. The culture of cats’ pancreas cells was obtained from the pancreas of cat fetuses. Cell culture process was carried out according the standard method in CO2- incubator. Glucose level in blood serum was determined by means of electrochemical analysis.
The results of the study. The authors studied the impact of allogenic transplantation cells culture of bone marrow, lipid tissue and pancreas on the course of experimentally generated diabetes mellitus in rats. The study revealed the decrease of glucose level in the blood of the animals under investigation at cell material transplantation. The most efficient culture for experimental diabetes mellitus therapy is the culture of pancreas cells which has become the cause of its further study in cats.
During allogenic transplantation cells culture of pancreas in cats the authors observed abrupt decrease of glucose level in the blood of the animals under investigation immediately after cell transplantation with the further approximation to the initial state.
The experimental models presented the positive effect of cell culture transplantation providing the grounds for the further implementation of this diabetes mellitus therapy method in the clinical practice.
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