Histological changes in dogs at coronaviral infection


  • V. Lisova
  • O. Dubinenko
Keywords: dogs, coronavirus infection, histological changes, serous-fibrinous jejuno-ileitis, hemorrhage, hyperplasia, lymphoid nodes, serous-hemorrhagic lymphadenitis

Abstract

The article presents the results of the study of histological changes in organs and tissues of dogs for coronavirus infection. The histological study of the pathological material of cadavers (n =5) dogs of different breeds and sex between the ages of 2 to 6 months, who died with diarrheal syndrome. The study of presence of coronavirus, without other assiociants, in these cases had previously been confirmed in the polymerase chain reaction analysis of fecal samples. The made histological sections were stained with hematoxylin and eosin according to standard prescriptions. The general histological structure and microstructural changes in histological preparations were studied under a light microscope. Histological studies have been confirmed and supplemented with pathoanatomical diagnoses, established after autopsies of dead bodies of dead animals. The most pronounced lesions and typical change in all dead dogs fixed contact in the small intestine (jejunum and to the ileum) and the regional lymph nodes to it, as well as in the spleen. It is shown that the morphological manifestations of coronavirus infection in dogs following features are on the microscopic level: the presence of exudative inflammation in the small intestine in the form serous-fibrinous jejuno-ileitis; hyperplasia of single and congested lymphoid nodes of the mucous membrane of the small intestine; hyperplasia and serous-hemorrhagic lymphadenitis mesenteric lymph nodes; foci of hemorrhage and hemorrhagic heart attacks in the spleen parenchyma; hyperplasia lymphoid nodes of the spleen; foci of hemorrhage in the serous membrane of the small intestine. Also, non-specific, but constant morphological features, which arose as a result of circulatory disorders and heart failure were: passive venous congestion of the liver and kidney; degenerative processes in the liver and kidney parenchyma. Consequently, local and general immunological processes develop during the coronaviral infection. Therefore, additional diagnostic markers appear hyperplastic and inflammatory changes of regional seats to the reproduction of the virus lymphoid organs.

Downloads

Download data is not yet available.

References

Godsall, S.A., Clegg, S.R., Stavisky, J.H. (2010). Epidemiology of canine parvovirus and coronavirus in dogs presented with severe diarrhoea to PDSA. Pet Aid hospitals. Veterinary research. 167, 196–201.

Radzykhovsʹkyy, M.L. (2016). Monitorynh enterytiv virusnoyi etiologiyi u sobak [Monitoring enteritis viral etiology in dogs].Scientific Messenger of National University of Veterinary Medicine and Biotechnologies named after S.Z. Gzhytsky. 18, 1(65), 138–142 (in Ukrainian).

Decaro, N., Buonavoglia, C. (2008). An update on canine coronaviruses: viral evolution and pathobiology. Veterinary Microbiology. 10, 132(3–4), 221–234.

Zaki, A.M., van Boheemen, S., Bestebroer, T.M., Osterhaus, A.D., Fouchier, R.A. (2012). Isolation of a Novel Coronavirus from a Man with Pneumonia in Saudi Arabia. N Engl J Med. 367, 1814–1820. Available at : http://www.nejm.org/doi/full/10.1056/NEJMoa1211721

Erles, K., Brownlie, J. (2008). Canine Respiratory Coronavirus: An Emerging Pathogen in the Canine Infectious Respiratory Disease Complex. Veterinary Clinics of North America: Small Animal Practice. 38(4), 815–825.

Decaro, N., Mari, V., von Reitzenstein, M. (2012). A pantropic canine coronavirus genetically related to the prototype isolate CB/05. Vet Microbiol. 159(1–2), 239–244.

Zappulli, V., Caliari, D., Cavicchioli, L. (2008). Systemic fatal type II coronavirus infection in a dog: Pathological findings and immunohistochemistry. Research in Veterinary Science. 84(2), 278–282.

Goralskij, L.P., Homych, V.T., Kononskij, O.I. (2011). Osnovy histologichnoyi tehniky i morfofunkcionalni metody doslidjen u normi ta pry patologiyi [Foundations of histological engineering and morphofunctional methods of research in norm and pathology]. Jytomir, Ukrainian: Polissya (in Ukrainian).

Abstract views: 48
PDF Downloads: 40
Published
2017-04-19
How to Cite
Lisova, V., & Dubinenko, O. (2017). Histological changes in dogs at coronaviral infection. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 19(78), 154-157. https://doi.org/10.15421/nvlvet7831