The characteristics of modern methods for the determination of raw protein in feeds and in plant materials
Todays in laboratory and manufacturing practice of veterinary medicine of Ukraine, for the detertimations of raw protein in feed and in plant raw are used as classic and as modern methods depending from the aim and the task of research. Thats why the article describes the modern methods of the determination of content of nitrogen and the calculation of raw protein in feeds for animals and in plant materials, in particular, photometric, classical – Kjeldahl method, modern – Dumas and spectrometric methods (the method of infrared spectrometry in the near range (NIR-spectrometry). Despite of the facts, that the Kjeldahl method remains generally accepted in the determination of the protein and is most often used in research, but the modern methods – the Dumas method and NIR-spectrometry are widely adopted in laboratory practice. The main advantage of these methods is the speed of the analysis (the one definition takes only a few minutes), practically there is no sample preparation, and the most important is that the toxic chemicals and catalysts are not used.
Thus, despite the fact that the Kjeldahl method remains generally accepted in the determination of the protein, and it is most frequently used in research, but modern methods, the Dumas method and NIR-spectrometry, are widely implemented in laboratory practice. The main advantage of these methods is the speed of conducting the analysis (one definition only takes a few minutes), there is practically no sample preparation, and most importantly, toxic chemicals and catalysts are not used.
Busenko, О.Т., Stolyk, В.D., Mogilnii, О.I. (2015). Technologia virobniztva produczii tvarinniztva: Pidruchnik. К.: Vischa osvita (in Ukrainian).
Кostenko, В.М., Panko, В.В., Sirovatko, К.М. (2008). Praktikum z godivli silskohospodarskich tvarin. Chimichnii sklad, оzinka pozhivnosti ta yakosti kormiv. Chastina І. Vinniza: RVB VDAO (in Ukrainian).
DSTU ISO 5983:2003. Коrmi dla tvarin (2005). Viznachenna vmistu azotu i obchislenna vmistu sirogo bilka. Меtod Kjeldahla (ISO 5983: 1997, IDT). Кiev, Derzhspozhyvstandard Ukraini (in Ukrainian).
GOST R 54390-2011. Producti pizhevii (2013). Оpredelenija obzheho soderzanija azota putem sziganija po medodu Duma i raschet soderzhanija belka. Chast 2. Zernovii, bobovie i molotie zernovie producti (ISO/TS 16634.2: 2004). Моskva, Standardinform (in Russian).
Bekker, Yu. (2009). Spectroskopija. М.: Technosphere (In Russian).
Sadchikova, N.P., Arzamastsev, A.P., Titova, A.V. (2008). Sovremennoe sostojanie problem primenenija IK-spectoskopii v pharmazevticheskom analize lekarstvennikh sredstv. Khimiko-Pharmacevtichnii zurnal. 8, 26–30 (In Russian).
Scheeren, T.W., Schober, L.P., Schwarte, L.A. (2012). Monitoring tissue oxygenation by near infrared spectroscopy (NIRS): background and current applications. Journal of Clinical Monitoring and Computing. 26(4), 279–287.
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