Тhe dynamics of the physiological state changes and hematological indicators of the rainbow trout during the launch of the biofilter in the installations of the closed water utilization


Keywords: nitrite, rainbow trout, biofilter, methaemoglobin, hypoxia, toxicity

Abstract

The purpose of the work was to investigate the effect of nitrites on the development of fry of rainbow trout, the number of red blood cells, the level of hemoglobin and methemoglobin during the launch of a biofilter installation of closed water use. In the water from the biofilter reactor, the nitrite content was determined using the GBL test. The fish blood was taken from the tail vein (after tail cropping) with a thinly drawn pasteurized pipette into a heparin test tube. In blood, the number of red blood cells, hemoglobin and methemoglobin levels were determined according to generally accepted methods. The results of changes in the physiological state and hematological parameters of rainbow trout in water of closed water supply facilities are presented. It is shown that during the launch of the biofilters of ultrasound, the greatest death of the rainbow trout occurs from 16 to 20 days of the experiment and is 14.9% of the total fish. At the same time, all live fish floated in the upper layers of water, in ≈ 50% of the fish there was a nervous tremor of the body and fins, it massively swam to the place of water supply, in ≈ 90% of the fish marked the darkening of the body and fins that were sealed, at 90% The fish color of the gills changed from red to brown. It was established that since the start of the biofilter and up to 25 days of the experiment in the blood of rainbow trout, the number of red blood cells decreased from 1.32 ± 0.12 T/L to 0.94 ± 0.08 T/liter. At the same time, a probable decrease in the investigated index in the blood of the fish was probable in the period from 15 to 20 days (P ≤ 0.05) and from 20 to 25 days (P ≤ 0.01). With the decrease of nitrite load on fish, 26 days after the start of the biofilter, due to the activation of denitrification microbes and the reduction of nitrite levels in water, the number of red blood cells in the blood increased, compared with the previous period, but did not return to the initial values even at the end of the experiment. The level of methemoglobin in the blood of rainbow trout since the start of the biofilter has increased and was directly related to the level of nitrites in the water biofilter. At the start of the biofilter, the level of methemoglobin in the blood of fish was the lowest and indicates that even low levels of nitrites in water cause the transfer of blood from a small trout of hemoglobin to the metform due to the oxidation of iron chemistry (Fe +2 ® Fe +3). At the 10th day of the study, its concentration in blood, compared with the start of the biofilter, increased by 8.8%, 15 days – by 15.0%, 20 days – by 26.4%, 25 days – by 16.8%, 30 day – by 12.8% and 35 days – by 2.1%. Such changes in the content of methemoglobin in the blood may indicate an enhanced leakage in erythrocytes of oxidative reactions involving active forms of oxygen and the possible development of oxidative stress in the body.

Downloads

Download data is not yet available.

References

Asi, A.A., Relve, P.F., & Herem, X.-Ja.Je. (1985). Opredelenie optimal'noj proizvoditel'nosti rybovodnoj ustanovki s zamknutym ciklom vo-doobespechenija. Industrial'noe rybovodstvo v zamknutyh siste-mah: sb. nauch. trudov. M.: VNIIPRH, 10–14 (in Russian).

Cherkesova, D.U., & Shahnazarova, A.B. (2002). Toksicheskoe vozdejstvie nitritov na organizm ryb v Stavropol'skom krae. Kavkazskij ornitologicheskij vestnik. 14, 108–111 (in Russian).

Chezhian, A., Senthamilselvan, D., & Kabilan, N. (2012). Histological changes induced by ammonia and ph on the gills of fresh water fish Cyprinus carpio var. communis (Linnaeus). Asian Journal of Animal and Veterinary Advances, 7, 588–596. doi: 10.3923/ajava.2012.588.596

Doblander, C., & Lackner, R. (1997). Oxidation of nitrite to nitrate in isolated erythrocytes :A possible mechanissm for adaptation to enviromental nitrite. Canadian Journal of Fisheries and Aquatic Sciences. 54(1), 157–161. doi: 10.1139/f96-254

Grebenjuk, T.V., & Konstantinenko, G.V. (2015). Metodi ochistki vodi na ribovodnih pіdpriєmstvah v umovah viroshhuvannja ribi v ustanov-kah zamknutogo vodopostachannja. Vіsnik NTUU «KPІ». 28, 110–114 (in Russian).

Grynevych, N. (2016). Osoblyvosti vykorystannia biofiltriv z riznymy typamy napovniuvacha v ustanovkakh zamknutoho vodopostachannia v akvakulturi [Features of biofilters with defferent types of filler plants in closed water acuaculture]. Scientific Messenger LNUVMBT named after S.Z. Gzhytskyj. 18, 3(70), 57–61 (in Ukrainian) doi: 10.15421/nvlvet7013.

Gutyj, B., Leskiv, K., Shcherbatyy, A., Pritsak, V., Fedorovych, V., Fedorovych, O., Rusyn, V., & Kolomiiets, I. (2017). The influence of Metisevit on biochemical and morphological indicators of blood of piglets under nitrate loading. Regulatory Mechanisms in Biosystems. 8(3), 427–432. doi: 10.15421/021766

Gutyj, B., Nazaruk, N., Levkivska, A., Shcherbatyj, A., Sobolev, A., Vavrysevych, J., Hachak, Y., Bilyk, O., Vishchur, V., & Guta, Z. (2017). The influence of ni-trate and cadmium load on protein and nitric metabo-lism in young cattle. Ukrainian Journal of Ecology, 7(2), 9–13. doi: 10.15421/2017_14

Hrynevych, N.Ie. (2017). Vmist nitryfikuiuchykh orhanizmiv u vodi reaktora biofiltra ustanovky zamknutoho vodopo-stachannia za vykorystannia riznykh typiv napovniuvacha. Naukovyi visnyk LNUVMBT imeni S.Z. Gzhytskoho. 19(82), 184–187 (in Ukrainian).

Hrynevych, N.Ye. (2017). Mikroorhanizmy protsesiv nitrohennoho tsyklu u vodi reaktora biofiltra v ustanovkakh za-mknutoho vodopostachannia za vykorystannia riznykh napovniuvachiv. Naukovyi visnyk veterynarnoi medytsyny. 1(133), 131–136 (in Ukrainian).

Hrynevych, N.Ye., & Dyman, T.M. (2016). Sezonni zminy hidrokhimichnykh pokaznykiv vody za vykorystannia ustanovok zamknutoho vodopostachannia dlia vyroshchuvannia raiduzhnoi foreli. Nauko-vyi visnyk veterynarnoi medytsyny. 2(130), 33–39 (in Ukrainian).

Huberuk, V., Gutyj, B., Gufriy, D., Binkevych, V., Hariv, I., Binkevych, O., & Salata, R. (2017). Impact of anti-oxidants on enzym activities of glutatione system of bulls bodies antioxidant defense under acute nitrate and nitrite toxicity. Scientific Messenger LNUVMBT named after S.Z. Gzhytskyj, 19(77), 220–224.

Hutyi, B.V. (2004). Do metodyky vyvchennia vplyvu nitrativ na stan antyoksydantnoi systemy bychkiv. Naukovyi visnyk Lvivskoi natsionalnoi akademii veterynarnoi medytsyny imeni S.Z. Gzhytskoho. 2, 48–52.

Khariv, M., Gutyj, B., Butsyak, V., & Khariv, I. (2016). Hematolohichni pokaznyky orhanizmu shchuriv za umov oksydatsiinoho stresu ta za dii liposomal’noho preparatu [Hematological indices of rat organisms un-der conditions of oxidative stress and liposomal preparation action]. Biological Bulletin of Bogdan Chmelnitskiy Melitopol State Pedagogical University, 6(1), 276–289 (in Ukrainian). doi: 10.15421/201615

Kushakovskij, M.S. (1970). Metgemoglobinemii. Spravochnik po funkcional'noj diagnostike. Medicina, 423–427 (in Russian).

Lapirova, T.B. (2016). Vlijanie nitrit-ionov na nekotorye pokazateli krovi plotvy (Rutilus rutilus L.) pri kratko-srochnom vozdejstvii. Voda: himija i jekologija. 2, 83–87 (in Russian).

Martyshuk, T.V., Gutyj, B.V., & Vishchur, O.I. (2016). Level of lipid peroxidation products in the blood of rats under the influence of oxidative stress and under the action of liposomal preparation of «Bu-taselmevit», Biological Bulletin of Bogdan Chmelnitskiy Melitopol State Pedagogical University. 6 (2), 22–27. doi: 10.15421/201631

Mazik, P.M., Hinman, M.L., Winkelmann, D.A., Klaine, S.J., Simco, B.A., & Parker N.C. (1991). Influence of Nitrite and Chloride Concentrations on Survival and Hematological Profiles of Striped Bass. Transactions of the American Fisheries Society. 120(2), 247–254. doi: 10.1577/1548-8659(1991)120<0247:IONACC>2.3.CO;2

Metodicheskie ukazanija po provedeniju gematologicheskogo obsledovanija ryb (1999). M.: Minsel'hozprod (in Russian).

Nazaruk, N.V., Gutyj, B.V., & Gufrij, D.F. (2015). Vplyv metifenu ta vitamiksu se na aktyvnist' ami-notransferaz syrovatky krovi bychkiv za nitratno–kadmijevogo navantazhennja. Naukovyj visnyk L'vivs'kogo nacional'nogo universytetu veterynarnoi' medycyny ta biotehnologij im. G'zhyc'kogo. 17, 1(1), 121–126 (in Ukrainian).

Nazaruk, N.V., Hutyi, B.V., Murska, S.D., & Hufrii, D.F. (2016). Vplyv metifenu ta vitamiksu SE na riven vitaminiv A i E u krovi bychkiv za nitratno-kadmiievoho navantazhennia. Visnyk Sumskoho ahrarnoho universytetu. Seriia «Veterynarna medytsyna». 6(38), 27–30 (in Ukrainian).

Opekunova, M.G. (2016). Bioindikacija zagrjaznenij: ucheb. Posobie. – SPb. – Izd-vo S.-Peterb. un-ta.

Saoud, I., Naamani, S., Ghanawi, J., & Nasser, N. (2014). Effects of Acute and Chronic Nitrite Exposure on Rabbitfish Siganus rivulatus Growth, Hematological Parameters, and Gill Histology. J Aquac Res Devel-opment. 5(6), 263. doi:10.4172/2155-9546.1000263

Staryk, L.I., Gutyj, B.V., Vasiv, R.O., Gufrij, S.D., & Murs'ka, D.F. (2012). Vplyv sukupnogo vvedennja pirydoksynu gidrohlorydu z askorbinovoju kyslotoju na biohimichni ta morfologichni pokaznyky krovi bychkiv pry gostromu nitratno-nitrytnomu toksykozi. Naukovyj visnyk L'vivs'kogo nacional'nogo universytetu veterynarnoi' medycyny ta biotehnologij im. G'zhyc'kogo. 14, 2(1), 306–312 (in Ukrainian).

Svobodova, Z., Machova, J., Poleszczuk, G., Hjda, J., Hamaakova, J., & Kroupova, H. (2005). Nitrite Poi-soning of Fish in Aquaculture Facilities with Water-recirculating Systems. Acta Veterinaria Brno. 74, 129–137. doi: 10.2754/avb200574010129

Tilak, K.S., Lakshmi, S.J., & Susan, T.A. (2002). The toxicity of ammonia, nitrite and nitrate to the fish, Catla catla (Hamilton). Journal of environmental biology. 23(2), 147–149.

Vedel, N.E., Korsgaard, B., & Jensen, F. (1998). Isolated and combined exposure to ammonia and nitrite in rainbow trout: effects on electrolyte status, blood res-piratory propertiers and brain glutamine/glutamate concentrations. Aquatic Toxicology. 41(4), 325–342 doi: 10.1016/S0166-445X(97)00071-4

Veldre, I.A., & Rooma, M.Ja. (1990). Toksicheskoe dejstvie nitritov na ryb. Jekologija. 1, 71–73 (in Rus-sian).

Weirich, C.R., Tomasso, J.R., & Smith, T.I.J. (1993). Toxicity of Ammonia and Nitrite to Sunshine Bass in Selected Environments. Journal of Aquatic Animal Health. 5(1), 64–72. doi: 10.1577/1548-8667(1993)005<0064:TOAANT>2.3.CO;2

William, M., Lewis, Jr., & Morris, D. (1986). Toxicity of nitrite to fish: a review. Transactions of the American Fisheries Society. 111, 183–195. doi: 10.1577/1548-8659(1986)115<183:TONTF>2.0.CO;2

Zorriehzahra, M.J., Hassan, M.D., Gholizadeh, M., & Saidi, A.A. (2010). Study of some hematological and biochemical parameters of Rainbow trout (Oncorhynchus mykiss) fry in western part of Mazandaran province, Iran. Iranian Journal of Fisher-ies Sciences. 9(1), 185–198. http://jifro.ir/article-1-32-en.html

Zubina, N.F. (1967). Dinamika nakoplenija gemoglobina v ontogeneze raduzhnoj foreli. Obmen veshhestv i biohimija ryb, 171–176 (in Russian).

Yamagata, Y., & Niwa, M. Nitrite toxicity to eels. Aguaculture. 27(1), 5–11

Abstract views: 124
PDF Downloads: 120
Published
2018-02-24
How to Cite
Grynevych, N. (2018). Тhe dynamics of the physiological state changes and hematological indicators of the rainbow trout during the launch of the biofilter in the installations of the closed water utilization. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 20(83), 19-24. https://doi.org/10.15421/nvlvet8304