To studying the development of technological stress in the organism of piglets during the influence of the feed additives «Praimiks Bionorm K»
The article is devoted to increasing the preserving and survival of piglets in conditions of industrial cultivation, where their organism is exposed to constant effects of adverse technological factors. The state of stress that occurs in this case leads to a decrease in the rate of growth and resistance of the young to a variety of diseases and can ultimately cause death of animals. One of the most promising directions for preventing the negative effects of stress and increasing the overall resistance of the piglet organism is the search, development and introduction of dietary supplements that increase physiological processes in the body, improve metabolism, increase the energy of growth, increase the yield of meat products. The purpose of the work was to find out the physiological state of a piglet organism during the period of weaning at the influence of the biologically active feed supplement «Praimiks Bionorm K» in the conditions of the SEPC «Komarnivsky». The research was carried out on piglets of 5 – 60-day-old age of the Poltava beef breed. Technological stress was the weaning of piglets at 40 days of age and the group maintenance of them with a change in the structure of the ration during the growing season. Piglets of the experimental group, together with the main feed, were additionally fed with «Praimiks Bionorm K» in the amount of 9.0 g/100 kg of feed (manufacturer – biotechnology company «Ariadna» Odessa). According to the results of the conducted research, it was found that the process of adaptation of the organism of the piglets of the control group to the stress isolation after 5 days was accompanied by an increase in blood hemoglobin concentration in 1.35 times (P < 0.01), the number of leukocytes – by 1.21 times (P < 0.05) at the expense of rodent and segmental neutrophils and monocytes – 1.54 times (P < 0.01), with a decrease in the number of eosinophils – 3.0 times (P < 0.001) and lymphocytes – 1.15 times (P < 0.01), compared with the period before weaning. 20 days after weaning in the K group, there was a decrease in the number of eosinophils in the blood by 2.25 times in the blood and lymphocytes by 1.25 times (P < 0.01), in the increase in the cellular and segmental neutrophils by 2.15 and 1.90 times, monocytes – by 1.85 times (P < 0.01) in comparison with the period before weaning. Neutrophilia, lymphopenia and eosinopenia were the most pronounced and persistent signs of stress in the piglets of the K group that arose out of the regulatory influence of glucocorticoids. While using biologically active feed supplement «Praimiks-Bionorm K» 5 days after weaning in the blood of piglets, the content of hemoglobin increased by 1.26 times; after 20 days its concentration increased by 1.22 times, as well as the increase in the number of leukocytes in 1.22 times (P < 0.01), neutrophils of rod-nuclei – 2.0 times, segmental ones – 1.37 times (P < 0.01), compared to control. The obtained results indicate that the use of BAFA «Praimiks Bionorm-K» allows to prevent the development of stressful events in the piglet organism, improve the status of the cellular level of nonspecific resistance and the level of productivity during the period of weaning and at different stages of development of the stress reaction after weaning.
Gutyj, B., Leskiv, K., Shcherbatyy, A., Pritsak, V., Fedorovych, V., Fedorovych, O., Rusyn, V. & Kolomiiets, I. (2017). The influence of Metisevit on biochemical and morphological indicators of blood of piglets under nitrate loading. Regulatory Mechanisms in Biosystems, 8(3), 427–432. doi: 10.15421/021766.
Stojanovskyj, V.G., Matsjuk, O.I., Кolotnytskyy, V.A., Kolomijets, I.A. (2016). Quantitative composition of the microflora of piglets intestinal in different periods of stress weaning under the using of additives «B–glucan» and «Biovir». Scientific Messenger LNUVMBT named after S.Z. Gzhytskyj. 18, 2(66), 176–179. doi:10.15421/nvlvet6635.
Gleimer, M. & Parham, Р. (2003). Stress Management: MHC Class I and Class I-like Molecules as Reporters of Cellular Stress. Biological Psychiatry. 19(4), 469-477. doi: doi: 10.1016/S1074-7613(03)00272.
Panin, A.N. & Malik, N.I. (2006). Probiotiki – neotemlemyj komponent racionalnogo kormlenija zhivotnyh. Veterinarija, 7, 3–6 (in Russian).
O'Mahony, S.M., Marchesi, J.R., Scully, P., Codling, C., Ceolho, A.M., Quigley, E.M., Cryan, J.F. & Dinan, T.G. (2009). Life Stress Alters Behavior, Immunity, and Microbiota in Rats: Implications for Irritable Bowel Syndrome and Psychiatric Illnesses. Biological Psychiatry. 65(3), 263–267. https://www.ncbi.nlm.nih. gov/pubmed/18723164.
Rider, P., Voronov, E., Dinarello, C.A., Apte, R.N. & Cohen, I. (2017). Alarmins: Feel the Stress. Journal of Immunology. 198(4), 1395–1402. doi: 10.4049/jimmunol.1601342.
Vlizlo, V.V., Fedoruk, R.S. & Ratіch, I.B. (2012). Laboratornі metody doslіdzhen u bіologіі, tvarynnyctvі ta veterinarnіj medycynі: dovіdnyk (in Ukrainian).
Frick, L.R., Rapanelli, M., Bussmann, U.A., Klecha, A.J., Arcos, M.L., Genaro, A.M., & Cremaschi, G.A. (2009). Involvement of Thyroid Hormones in the Alterations of T-Cell Immunity and Tumor Progression Induced by Chronic Stress. Biological Psychiatry. 65(11), 935–942. https://www.ncbi.nlm.nih.gov/ pubmed/19167703.
Nallagatla, S.R., Toroney, R., & Bevilacqua, P.C. (2011). Regulation of innate immunity through RNA structure and the protein kinase PKR. Current Opinion in Structural Biology, 21(1), 119–127. doi: 10.1016/j.sbi.2010.11.003.
Kogelberg, H. & Feizi, Т. (2001). New structural insights into lectin-type proteins of the immune system. Current Opinion in Structural Biology. 11(5), 635-643. doi: 10.1016/S0959-440X(00)00259-1.
Snodgrass, R.G., Huang, S., Namgaladze, D., Jandali, O., Shao, T., Sama, S., Brüne, B., & Hwang, D.H. (2016.). Docosahexaenoic acid and palmitic acid reciprocally modulate monocyte activation in part through endoplasmic reticulum stress.The Journal of Nutritional Biochemistry. 32, 39–45. doi: 10.1016/j.jnutbio.2016.01.010.
Murray, P.J. & Wynn, T.A. (2011). Protective and pathogenic functions of macrophage subsets. Nature Reviews Immunology. 11, 723–737. doi: 10.1038/nri3073.
This work is licensed under a Creative Commons Attribution 4.0 International License.