The study of essential oil on antimicrobial activity and their composition towards microorganism


Keywords: essential oils, microorganisms, benzalkonium chloride, nanoparticles, bactericidal agent.

Abstract

In the course of the research, the microorganisms that often caused disease for animals were used, according to the literature, were genetically predisposed to resistance to traditional antibiotics and needed to find new effective means to combat them. It was used different antibiotic substances benzalkonium chloride, silver nanoparticles, and promising in this direction, essential oils of plants: fir, eucalyptus. The article provides information on the selection and study of the efficacy of substances, components with essential oils necessary for the development of complex bactericidal agents and the study of their antimicrobial activity against microorganisms. In determining the antimicrobial activity of essential oils, test cultures were used: Microccoccus luteus ATCC9341, Microccoccus lisogenicus, B. subtilis AATCC6633, B.cereus ATCC6633, S. aureus 209-P, S. zooepidemicus, Clebsiella spp, Salmonella cholerae suis, Pasterella multocida from the museum of State Scientific and Control Institute of Biotechnology and strains of microorganisms. The determination of fungicidal acity was performed on microscopic molds of Penicillium citrinum, Penicillium urticae, Aspergillus flavus, Aspergillus ochraceus. In experiments with Staphilococcus aureus, Streptococcus zooepidemicus, Pasteurella multocida, the antibiotic action of essential oils of eucalyptus and puff was confirmed and the possibility of its enhancement by the addition of quaternary ammonium compounds was confirmed. According to the results, the use as an alternative to antibiotics and disinfectants of preparations based on vegetable essential oils in the form of aerosols not only improves the number of animals from respiratory infections of bacterial origin, but also significantly improves the ecological conditions of the work of personnel in livestock buildings, promotes treatment and prevention respiratory diseases. The investigations have proved the expediency of using vegetable essential oils for the design of highly effective drugs and the possibility of enhancing their antimicrobial activity by quaternary ammonium compounds and silver nanoparticles, as well as a complex preparation «Asept» for aerosol application in the presence of animals.

References

Boiko, O.P., Sen, O.M., Boiko, P.K., Kurtiak, B.M., Pundiak, T.O., & Sobko, G.V. (2017). Characteristics of morphological signs and physiological properties of salmonel stems, isolated from birth and television. Scientific Messenger LNUVMB, 19(78), 129–135. doi:10.15421/nvlvet7826.

Gutyj, B., Grymak, Y., Hunchak, V., Mysak, A., Nazaruk, N., Brezvyn, O., Hariv, I., Shcherbatyy, A., Semeniv, B., Bushueva, I., Parchenko, V., & Kaplaushenko, A. (2018). Preclinical searches of the preparation Thireomagnile. Ukrainian Journal of Ecology. 8(1), 688–695. doi: 10.15421/2018_267.

Hanaki, H., & Hiramatsu, K. (2001). Detection methods of glycopeptide-resistant Staphylococcus aureus. Susceptibility testing. Methods in Molecular Medicine. 48, 85–91 doi: 10.1385/1-59259-077-2:85.

Homzykov, O.M. (2006). Profilaktyka ta likuvannia hostrykh respiratornykh zakhvoriuvan teliat aerozoliamy aktyvnykh biolohichnykh preparativ. Epizootolohiia i profilaktyka infektsiinykh khvorob velykoi rohatoi khudoby: Mizhnarodna nauk.-prakt. konf.: Tezy dopovidei. K.: NAU, 25 (in Ukrainian).

Kovalenko, V.L., & Nedosiekov, V.V. (2011). Kontseptsiia rozrobky ta vykorystannia kompleksnykh dezinfektantiv dlia veterynarnoi medytsyny: Monohrafiia (in Ukrainian).

Kurtyak, B.M., Romanovych, M.S., Pundyak, T.O., Romanovych, М.М., Romanovych, L.V., & Sobko, G.V. (2017). Ecological features of episotic processes. Scientific Messenger LNUVMB, 19(78), 108–111. doi:10.15421/nvlvet7822.

Kysera, Ya.V., Storchak, Yu.G., & Gutyj, B.V. (2018). Experimental study of immunoprophylactic anti-pneumococcal medicine and its immunogenic properties. Ukrainian Journal of Ecology. 8(1), 307–316. doi: 10.15421/2018_216.

Levkivska, N., Gutyj, B., & Levkivskyj, D. (2016). Comparative effectiveness therapeutic and prophylactic preparations when applying 3% of ethanol–water emulsion of propolis and antibiotics for catarrhal pneumonia in calves. Scientific Messenger LNUVMBT named after S.Z. Gzhytskyj. 18, 2(66), 116–121. doi:10.15421/nvlvet6624.

Lorian, V. (1996). Antibiotics in laboratory medicine. Baltimore: Williams and Wilkins.

Vasilyeva, T.B. (2016). The monitoring of epizootic situation of colibacteriosis in Ukraine during 2004 – 2015. Scientific Messenger LNUVMBT named after S.Z. Gzhytskyj, 18, 2(66), 30–34 doi:10.15421/nvlvet6607.

Weidemann, B. (1998). Evaluation of data from susceptibility testing. International journal of antimicribial agents. 10, 218–219.
Published
2018-04-26
How to Cite
Kovalenko, V., Napnenko, O., Chornyi, I., & Zagrebelnyi, A. (2018). The study of essential oil on antimicrobial activity and their composition towards microorganism. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies, 20(87), 38-41. https://doi.org/10.15421/nvlvet8707