The resistance of isolated bacteria out of the dental plaque of dogs to antibiotics
The dental plaque may be defined as a biotope of the oral cavity, where the microflora exists in two versions: parietal and cavitary (planktonic). It represents a biofilm in which associations of microorganisms are gathered in microcolonies which are surrounded by a protective matrix and are attached to a biotic or abiotic surface. The water channels go through the biofilm and carry nutrients and products of microorganisms' vital functions are washed away by the flow of saliva. Microorganisms in a biofilm demonstrate high resistance to antimicrobial agents because of the fact that substances only with low molecular weight are allowed to pass through. Therefore, the aim of the research was to determine the resistance of planktonic and biofilm microflora isolated out of the dental plaque of dogs with chronic catarrhal gingivitis to antibiotics. Research materials include the washings out of the teeth selected by a sterile cotton swab, which was put into a 1cm3 test tube of the sterile solution with 0.5% mass fraction of sodium chloride. Primary sowings of the material for the detection of Micrococcus and Staphylococcus were carried out on MPA containing 7% sodium chloride and 5% of the blood of cattle, Streptococcus – on the Garro environment, Enterococcus – on Enterococagar, Corynebacterium – on MPA with 5% of the blood of cattle, Acinetobacter – on the King B environment for not fermented microorganisms and was grown at 37°C, Pseudomonas spp. – on the environment which contains 0.2% of N-cetylperdine chloride, E. coli – on the Endo environment. Identification of the selected microorganisms was carried out due to the determinant of bacteria Berge. Sensitivity of the selected microorganisms to antibiotics was researched by the Kirby Bauer Disk Diffusion Method, and its sensitivity to microorganisms in the biofilm by Stewart P.S. It was found that among the planktonic forms of the dental plaque microorganisms were sensitive to ampicillin + sulbactam – 91.0, enrofloxacin – 82.0, tylosin – 83.3, ceftiofur – 79.3, vancomycin – 75.2, gentamicin and doxycycline – 66.2, cefazolin – 64.6, ceftriaxone – 64.0, oxacillin – 63.5 and cefuroxime – 60.0% of cultures.Oxacillin, tylosin, cefazolin and enrofloxacin are recommended to reduce the risk of bacterial antimicrobial resistance in case of the detection of Staphylococcus spp. in dental plaques, ampicillin + sulbactam, ceftiofur – in case of the detection of Corynebacterium spp., cefuroxime and ceftriaxone in case of the detection of Streptococcus spp., gentamicin – Micrococcus spp., vancomycin – Enterococcus spp. and doxycycline – in case of the detection of Acinetobacter spp. in dental plaques.
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