Biofilm-forming ability of coccus forms of the caecal microflora of laying hens when using the probiotic and nanonutrition cobalt


Keywords: laying hens, cecum, nanocobalt, probiotic, biofilm, Staphylococcus spp., Enterococcus spp., Streptococcus spp.

Abstract

The use of the feed supplement on the basis of probiotic microorganisms of the genus Lactobacillus in combination with nano-cobalt preparations in a dose of 0.08 mg/kg liveweight in laying hens caused the most significant reduction in the proportion of cow's forms of the microflora of the colon (Staphylococcus spp., Enterococcus spp., Streptococcus spp.) that formed high-density biofilms by increasing the percentage of these microorganisms with low and medium biofilm-forming ability. After 14 days of use of probiotic and nanocobalt at a dose of 0.08 mg/kg, an increase in the percentage of Staphylococcus spp. microorganisms, which formed low and medium density biofilms, respectively, was 7.2 and 18.2%, due to the reduction of those with high biofilm formation ability. Such a redistribution of the ability of the studied microorganisms to form a biofilm remained after 28 days of the experiment. However, the number of microorganisms of Staphylococcus spp., which formed low density biofilms, was maximum i.e. 46.2%. Instead, the number of microorganisms of Staphylococcus spp., which formed high density biofilms, was minimal and amounted to 12.6%. The indicated trend persists after the end of application of the additive after 14 days. When using probiotic and nano-cobalt at a dose of 0.08 mg/kg, the highest reliability (Р < 0.001) of changes in the bio-film-forming ability of microorganisms Enterococcus spp. was established, namely, its reduction. At the same time, the percentage of microorganisms that formed low-density biofilms was the highest in 28 days of use (by 5.1%) and 14 days after the end of feeding of the additive (by 7.1%). At the same time, the percentage of microorganisms Enterococcus spp. with a high biofilm-forming ability gradually decreased and reached a minimum of 28 days of the experiment (5.6%). On the 14th day after stopping the feeding of the supplement, it reached 9.8%. With an increase in the dose of nanocobalt up to 0.8 mg/kg, significant changes were observed only on the 28th day of feeding and 14 days after the end of the feeding of the feed additive, namely: a decrease in the number of microorganisms Enterococcus spp. with a high biofilm production capacity of 3.4% and 4.8%, respectively. Regarding microorganisms of Streptococcus spp. the most visible effect could be observed with the use of probiotic in a complex with nano-cobalt in a dose of 0.08 mg/kg, namely: by 17.4%, the number of microorganisms with high bio-plating ability with a gradual increase in the percentage of those that had a low (10.2%) and average (by 7.2%) biofilm capacity. After the application of the suppressant was discontinued for 14 days, the corrected changes were maintained.

References

Pavlova, N.V., Kirzaev, F.S., & Lapinskajte, P. (2006). The value of intestinal normal microflora of birds for their organism. H. zootech. 10, 37–40.

Scupham, A.J. (2007). Succession in the intestinal microbiota of preadolescent turkeys. FEMS Microbiology Ecology. 60(1), 136–147. doi: 10.1111/j.1574-6941.2006.00245.x

Vecherskii, M.V., Kuznetsova, T.A., Kostina, N.A., Gorlenko, M.V., Golichenkov, M.B., Umarov, M.M., & Naumova, E.I. (2014). Role of microbiocenosis of the gastrointestinal tract in the nutrition of grouse. Biology Bulletin. 41(3), 281–285. doi: 10.1134/S1062359014030108.

Round, J.L., & Mazmanian, S.K. (2009). The gut microbiota shapes intestinal immune responses during health and disease. Nat Rev Immunol. 9(5), 313–323. doi: 10.1038/nri2515.

Lukovsʹka, O.I. (2015). Mikrobotsenoz kyshechnyku perepeliv porody «Faraon» za vykorystannya preparativ «Activo» i «Propoul». Biolohiya tvaryn. 17(4), 181 (in Ukrainian).

Scupham, A.J. (2009). Campylobacter colonization of the Turkey intestine in the context of microbial community development. Appl. Environ. Microbiol. 75(11), 3564–3571. doi:10.1128/AEM.01409-08.

Lee, Y.K., & Mazmanian, S.K. (2010). Has the microbiota played a critical role in the evolution of the adaptive immune system? Science. 330(6012), 1768–1773. doi: 10.1126/science.1195568.

Stanley, D., Hughes, R.J., & Moore, R.J. (2014). Microbiota of the chicken gastrointestinal tract: influence on health, productivity and disease. Appl. Microbiol. Biotechnol. 98(10), 4301–4309. doi: 10.1007/s00253-014-5646-2.

Mohd Shaufi, M.A., Sieo, C.C., Chong, C.W., Gan, H.M., & Ho, Y.W. (2015). Deciphering chicken gut microbial dynamics based on high-throughput 16S rRNA metagenomics analyses. Gut Pathogens. 7, 4. doi: 10.1186/s13099-015-0051-7.

Dibner, J.J., Richards, J.D., & Knight, C.D. (2008). Microbial imprinting in gut development and health. J Appl Poult Res. 17(1), 174–188. doi: 10.3382/japr.2007-00100.

Kaminsʹka, M.V., Stefanyshyn, O.M., Huralʹ, S.V., Popyk, I.M., Ponkalo, L.I., & Boretsʹka, N.I. (2015). Osoblyvosti formuvannya mikrobotsenozu kyshkovyka pekinsʹkykh broylernykh kachok. Silʹsʹkohospodarsʹka mikrobiolohiya. 21, 72–76 (in Ukrainian).

Baldi, F., Bianco, M.A., Nardone, G., Pilotto, A., & Zamparo, E. (2009). Focus on acute diarrhoeal disease. World J. Gastroenterol. 15(27), 3341–3348. doi: 10.3748/wjg.15.3341.

Kukhtyn, M., Berhilevych, O., Kravcheniuk, K., Shynkaruk, O., Horiuk, Y., & Semaniuk, N. (2017). The influence of disinfectants on microbial biofilms of dairy equipment. EUREKA: Life Sciences. 5, 11–17. doi: 10.21303/2504-5695.2017.00423.
Published
2018-04-26
How to Cite
Turko, I., & Ushkalov, V. (2018). Biofilm-forming ability of coccus forms of the caecal microflora of laying hens when using the probiotic and nanonutrition cobalt. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies, 20(87), 60-64. https://doi.org/10.15421/nvlvet8712