Sensitivity of L. casei and E. сoli to active substances of anti-parasitic preparations
The data of the influence of active substances of anthelmintic and antiprotozoal preparations on the growth of L. casei IMB B-7280 and E. coli 055K59 are provided in the article. Their minimal inhibitory concentrations were determined for these strains of microorganisms and the active substances with which possible simultaneous application of probiotic strain L. casei IMB В-7280 is established. With this aim, the effect on the growth of L. casei IMB B-7280 and E. coli 055K59 and the minimum inhibitory concentration of fenbendazole, levamisole and ivermectin, which are part of the anthelmintic preparations and amprolium, tylosin, sodium sulfadimexone and sodium sulfatyazole, which are active substances of antiprotozoal drugs, were investigated. The determination of the minimum inhibitory concentration of the active substances of antiparasitic agents against these strains of microorganisms was carried out in in vitro experiments by serial dilutions in a dense MRS environment and MPA, and a study of the effect on the growth by diffusion method, followed by measurement of growth retardation zones in millimeters. It is established that among active substances of anthelmintic preparations only phenbendazole caused growth retardation and only relative to L. casei IMB B-7280. Among the active substances of antiprotozoal drugs, sodium sulfatyazole was the most active, which inhibited growth as L. casei IMB-7280 and E. coli 055K59 № 3912/41. Thylosin was effective only in relation to L. casei IMB B-7280 and at the highest concentration of 0.03%, the growth retardation zone was 23.4 ± 0.92 mm. Sodium sulfadimetoxin caused the growth retardation of L. сasei IMB В-7280 only at the highest concentration. The minimum inhibitory concentration of active substances of anti-parasitic drugs was different for strains L. casei IMB B-7280 and E. coli 055K59 № 3912/4. The strongest inhibitory effect was shown by tylosin, which stopped the growth of L. casei IMB B-7280 and E. coli 055K59 № 3912/41 respectively at concentrations of 0.00125 and 50.0 mg/ml. Active substances such as amprolium, levamisole and ivermectin did not significantly inhibit the growth of L. casei, IMB B-7280 and E. coli 055K59 № 3912/41, since their minimal inhibitory concentration was in the range of 4000 to 6000 mg ml.
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