Studying of disinvasion action of the disinfectant Virosan for eggs Nematodes genus Trichuris parasitizing in sheep


Keywords: disinvasion, test-culture of eggs, sheep, Trichuris оvis, Trichuris skrjabini, Trichuris globulosa

Abstract

Today sheep breeding in the world is gaining important socio-economic significance. In the conditions of the Ukrainian market, the sector provides the population with food products, industry – raw materials for production. One of the criteria for the efficiency of the industry is the epizootic well-being of farms in relation to invasive diseases, especially the most common ones – nematodoses of the gastrointestinal tract of sheep, which cause areas of significant economic losses. One of the factors of transmission of invasive diseases are objects of the environment, contaminated with eggs and larvae of pathogens of parasitic diseases. Therefore, the disinvasion of these sites is considered one of the decisive measures in the fight against parasites, since the destruction of the invasive elements in the external environment leads to a break in the epizootic chain of parasitic diseases of sheep, including trichurosis. Due to the presence of species diversity of nematodes of the genus Trichuris, parasitizing in sheep, high resistance of their exogenous stages of development to the action of environmental factors and chemicals, it is important to study the disinvasive effect of modern chemical agents on eggs of trichurosis pathogens. The purpose of the work was to study in a laboratory the disinvasive properties of the means of domestic production “Virosan” in the case of eggs of nematodes of the genus Trichuris parasitizing in sheep. The results of the research revealed that Virosan (LTD “BioTestLab”, Ukraine), using its solutions in 0.5% and 1% concentrations for exposures of 10–60 minutes, exhibited pronounced disinvasive properties (DE – 91.80–100% respectively) in relation to the test culture of eggs nematodes of the genus Trichuris, isolated from sheep. The investigated drug causes destructive changes in the form of destruction of the egg shell on one side of the poles and the termination of the development of the embryo at various stages. It has been experimentally established that the use of the means for the test culture of eggs of trichuryses of sheep of different species has its own differences. High level of disinfestation efficiency is registered for the following modes of application of the product: for the species Trichuris globulosa – a solution of 0.25% concentration (exposure of 60 minutes), as well as 0.5% and 1.0% concentration (exposures 10, 30 and 60 minutes), DE is 93.48–100.00%; for the species T. skrjabini – a solution of 0.5% and 1.0% concentration for all proposed exposures DE is 92.31–100.00%; For the species T. ovis – for use of 0.5% and 1.0% of working solutions (exposure 30, 60 and 10–60 minutes) DE is 90.59–100.00%.

References

Baliuk, S., Medvedev, V., Kucher, A., Solovey, V., Levin, A., & Kolmaz, Y. (2017). Ukrainian chernozems as a factor in global food security and resilience of agriculture to climate change. Global symposium on soil organic carbon, 21–23 March 2017. Rome, Italy. http://www.fao.org/3/a-bs034e.pdf.

Braga, F.R., Araújo, J.V, Milani, J.A., Carvalho, R.O., Silva, A.R., Campos, A.K., & Tavela, A.O. (2008). Ovicidal activity of Paecilomyces lilacinus on Moniezia sp. eggs. Journal of Helminthology, 10, 241–243. doi: 10.1017/S0022149X08977488.

Braga, F.R., Araújo, J.V., Carvalho, R.O., Silva, A.R., Araujo, J.M., Tavela, A.O., Costa, P.R.S., & Campos, A.K. (2009). Ovicidal activity of different concentrations of Pochonia chlamydosporia chlamydospores on Taenia taeniaeformis eggs. World Journal of Microbiology and Biotechnology, 25(3), 533–535. doi: 10.1007/s11274-008-9920-2.

Brewster, J., Oleszkiewicz, J., Bujoczek, G., Reimers, R.S., Abu-Orf, M., Bowman, D., & Fogarty, E. (2003). Inactivation of Ascaris suum eggs in digested and dewatered biosolids with lime and fly ash at bench scale and full scale. Journal of Environmental Engineering and Science, 2, 395–400. doi: 10.1139/S03-041.

Byrka, V.I., Prykhodko, Yu.O., Mazannyi, O.V., & Hilieva, M.I. (2013). Osoblyvosti epizootolohii, diahnostyka ta borotba z trykhurozom i suputnimy invaziiamy dribnoi rohatoi khudoby pry sumisnomu utrymanni. Naukovi pratsi Pivdennoho filialu Natsionalnoho universytetu bioresursiv i pryrodokorystuvannia Ukrainy “Krymskyi ahrotekhnolohichnyi universytet”, 151, 136–143. http://nbuv.gov.ua/UJRN/Npkau_2013_151_24 (in Ukrainian).

Cherepanov, A.A., Kumbov, P.K., Grigor'ev, A.G., & Skira, V.N. (1999). Metodicheskie rekomendacii po ispytaniju i primeneniju sredstv dezinvazii v veterinari. Moskva: Rossijskaja akademija sel'-skohozjajstvennyh nauk (in Russian).

Cutillas, C., German, P., Arias, P., & Guevara, D.C. (1995). Trichuris ovis and Trichuris globulosa: morphological, biometrical and genetic studies. Experimental Parasitology, 81, 621–625. doi: 10.1006/expr.1995.1159.

Dubná, S., Langrová, I., Jankovská, I., Vadlejcha, J., Pekárb, S., Nápravníka, J., & Fechtner, J. (2007). Contamination of soil with Toxocara eggs in urban (Prague) and rural areas in the Czech Republic. Veterinary Parasitology, 144(1–2), 81–86. doi: 10.1016/j.vetpar.2006.09.023.

Evstaf'eva, V.A., Mel'nichuk, V.V., Sharavara, T.A., Sirenko, E.V., Makarevich, N.A., Kucenko, Ju.P., & Hlevnaja, G.S. (2018). Osobennosti jembrional'nogo razvitija jaic nematod Trichuris skrjabini (Baskakov 1924), parazitirushhih u ovec. Agrarnaja nauka Evro-Severo-Vostoka, 1(62), 65–69. doi:10.30766/2072-9081.2018.62.1.65-69. (in Russian).

Gaasenbeek, C.P.H., & Borgsteede, F.H.M (1998) Studies on the survival of Ascaris suum eggs under laboratory and simulated field conditions. Veterinary Parasitology. 5(2–3), 227–234. doi: 10.1016/S0304-4017(97)00198-2.

Goncharenko, I.V., Vinnichuk, D.T., & Bogdanova, N.V. (2018). Sochetanie molochnogo skotovodstva i ovcevodstva ‒ real'naja perspektiva ili vynuzhdennaja mera? Ukrainskij zhurnal jekologii, 8(1), 838–844. doi: 10.15421/2017_282. (in Russian).

Hinks, M.I., & Thomas, R.J. (1974). A new record of the occurrence of Trichuris skrjabini Baskakov, 1924 in sheep in Britain. Journal of Helminthology, 48(1), 33–38. doi: 10.1017/S0022149X00022574.

Katakam, K.K., Mejer, H., Dalsgaard, A., Kyvsgaard, N.C., & Thamsberg, S.M. (2014). Survival of Ascaris suum eggs in liquid manure at different ammonia concentrations and temperatures. Veterinary Parasitology, 204, 249–257. doi: 10.1016/j.vetpar.2014.05.017.

Klymenko, O.S., Mykhailiutenko, S.M., Kruchynenko, O.V., & Lytvynenko, A.I. (2018). Porivniannia terapevtychnoi efektyvnosti ta dezinvaziinoi aktyvnosti preparativ za helmintoziv svynei u vyrobnychykh umovakh pryvatnykh hospodarstv Poltavskoi oblasti. Visnyk sumskoho natsionalnoho universytetu: Seriia Veterynarna medytsyna, 1(42), 116–120 (in Ukrainian).

Kuchai, J.A., Chishti, M.Z., Zaki, M.M., Ahmad, J., Ra-sool, M., Dar, S.A., & Tak, H. (2011). Prevalence of nematode parasites in Sheep of Ladakh-India. Jour-nal of Agricultural Extension and Rural Development, 3(13), 229–231. doi: 10.5897/JAERD11.099.

Kucher, L. (2018). Estimation of potasium reserves in zonal chernozemic soils of Ukraine’s forest-steppe. Polish Journal of Soil Science, 51(1), 83–91. doi: 10.17951/pjss/2018.51.1.83.

Mamedova, M.M. (2011). Zagrjaznennost' jajcami trihocefaljusov pochv zapadnoj chasti Azerbajdzhana. Agrarnaja nauka Azerbajdzhana, 2, 195–198 (in Russian).

Mamedova, M.M. (2012). Vyzhivaemost' jaic trihoce-faljusov v pochve, udobrennoj svezhim ovech'im navozom. Trudy оbshhestva zoologov Azerbajdzhana, 2, 129–133 (in Russian).

Mamedova, M.M., & Fataliev, G.G. (2006). Razvitie jaic Trichocephalus ovis v razlichnyh tipah pochv. Veterinarnaja medicyna, 4, 28–32 (in Russian).

Melnychuk, V.V., & Yuskiv, I.D. (2018). Trykhuroz svynei: monohrafiia. Ukrpromtorhservis, Poltava (in Ukrainian).

Moazeni, M., Saadaty Ardakani, Z.S., Saharkhiz, M.J., Jalaei, J., Khademolhoseini, A.A., Shams Esfand Abad, S., & Mootabi Alavi, A. (2017). In vitro ovicidal activity of Peganum harmala seeds extract on the eggs of Fasciola hepatica. Journal of Parasitic Diseases, 41(2), 467–472. doi: 10.1007/s12639-016-0830-1.

Mun, S., Lee, Y.-W., & Yoon, J. (2012). Ovicidal activities of supercritical CO2 and N2O on Ascaris suum eggs. Journal of Industrial and Engineering Chemistry, 18(1), 504–508. doi: 10.1016/j.jiec.2011.11.032.

Naidoo, D., Archer, C., Louton, B., & Rodda, N. (2016). Testing household disinfectants for the inactivation of helminth eggs on surfaces and in spills during pit latrine emptying. Water S.A. 42(4), 560–570. doi: 10.4314/wsa.v42i4.06.

Nasonova, M.V. (1974). Ustojchivost' jaic vlasoglavov svinej k vozdejstvijurazlichnyh faktorov vneshnej sredy. Nauchnye trudy voronezhskogo sel'-skohozjajstvennogo instituta, 60, 55–60 (in Russian).

Olehnovich, N.I., & Jatusevich, A.I. (2011). Trihocefalez svinej: monografija. Vitebsk: Vitebskaja gosudarstvennaja akademija veterinarnoj mediciny (in Russian).

Pecson, B.M., & Nelson, K.L. (2005). Inactivation of Ascaris suum Eggs by Ammonia. Environmental Science & Technology, 39(20), 7909–7914. doi: 10.1021/es050659a.

Prokopenko, K.O., & Udova, L.O. (2017). Silske hospodarstvo Ukrainy: vyklyky i shliakhy rozvytku v umovakh zminy klimatu. Ekonomika i prohnozuvannia. 1, 92–107. doi: 10.15407/eip2017.01.092. http://nbuv.gov.ua/UJRN/econprog_2017_1_8. (in Ukrainian).

Rocha, G.C., Harter-Lailheugue, S., Le Bailly, M., Ara´ujo, A., Ferreira, L.F., Serra-Freire, N.M., & Bouchet F. (2006). Paleoparasitological remains revealed by seven historic contexts from “Place d'-Armes”, Namur, Belgium. Memórias do Instituto Oswaldo Cruz – Rio de Janeiro, 101(2), 43–52. doi: 10.1590/S0074-02762006001000008.

Romero, N.C., Mendoza, G.D., Bustamante, L.P., Yanez, S., & Ramirez, N. (2010). Contamination and viability of Toxocara sp. in feces collected from public parks, streets and dogs in Tejupilco at the subhumid tropic of Mexico. Journal of Animal and Veterinary Advances, 9(23), 2996–2999. doi: 10.3923/ javaa.2010.2996.2999.

Shamhalov, M.V., Adzieva, H.M., & Shamhalov, V.M. (2007). Izuchenie srokov razvitija i vyzhivaemosti jaic i lichinok trihocefal vo vneshnej srede v jeksperimental'nyh uslovijah ravninnoj zony Dagestana. Jug Rossii. Jekologija, razvitie, 4, 75–78 (in Russian).

Stets, G. & Voloshyna, N. (2016). Toxocara canis – bioindicator of parasitic soil contamination of technogenically transformed territories. The Advanced Science Journa, 2016(03), 41–44. doi: 10.15550/ASJ.2016.03.041.

Voloshyna, N.O. (2010). Vyznachennia efektyvnosti nanochastok mahniiu na yaitsia Ascaris suum (Goeze, 1782) na test-obiektakh. Visnyk Lvivskoho universytetu. Seriia biolohichna, 52, 163–166.

Von Son-de Fernex, E., Alonso-Díaz, M.A., Mendoza de Gives, P., Valles-de la Mora, B., Zamilpa, A., & González-Cortazar, M. (2016). Ovicidal activity of extracts from four plant species against the cattle nematode Cooperia punctata. Veterinaria México OA. 3(2), 1–14. doi: 10.21753/vmoa.3.2.365.

Yevstafieva, V.O. (2009). Vyprobuvannia dezinfektantiv za askaroznoi invazii svynei. Visnyk Poltavskoi derzhavnoi ahrarnoi akademii. 1, 101–103 (in Ukrain-ian).

Yevstafieva, V.А., Yuskiv, I.D., & Melnychuk, V.V. (2015). An Investigation of Embryo and Eggshell De-velopment in Trichuris suis (Nematoda, Trichuridae) under Laboratory Conditions. Vestnik zoologii, 50(2), 173–178. doi: 10.1515/vzoo-2016-0020.

Yuskiv, I.D., & Melnychuk, V.V. (2015). Efektyvnist vykorystannia riznykh test-kultur yaiets helmintiv shchodo vstanovlennia dezinvaziinykh vlastyvostei khimichnykh zasobiv. Visnyk Poltavskoi derzhavnoi ahrarnoi akademii, 4, 58–60 (in Ukrainian).
Published
2018-09-08
How to Cite
Melnychuk, V., & Yuskiv, I. (2018). Studying of disinvasion action of the disinfectant Virosan for eggs Nematodes genus Trichuris parasitizing in sheep. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies, 20(88), 16-23. https://doi.org/10.32718/nvlvet8803