Circulating microflora and its resistance to antibacterial medications in the conditions of Farm “Pchani-Denkovich” of Zhydachiv district of Lviv region
The importance of the influence of microorganisms on the health of humans and animals is beyond doubt. In recent decades, a large amount of data on the interaction of the flora with the elements of the immune system has been accumulated. Therefore, it is important to identify any hazardous factors that must be prevented or neutralized. One of such factors is the circulating flora of the premises, the organism of the animal, its virulence and resistance to antibacterial drugs. Bacteriological studies included bacteriological culture on the nutrient environment, their identification and the study of antibioticsensitivity. According to the results of bacteriological studies of milk samples, it was found that in 25% of the studied samples Staphylococcus aureus cultures were found in different concentrations. In 4 samples, Proteus vulgaris was detected. Bacteriological studies of vaginal exudate from the cows after calving have shown that they have Escherichia coli, Escherichia coli haemolitica, Staphylococcus aureus, Streptococcus haemolyticus, Staphylococcus epidermidis, Enterobacter cloacae, Proteus vulgaris, mold fungi of the Aspergillus spp. The studies of the exudate from the nasal passages and the mouthof the calves found that all the tested samples contained Escherichia coli, Proteus vulgaris, Staphylococcus epidermidis, Enterococcus spp., Klebsiella pnemoniae and Aspergillus and Candida fungi. The results of calf excrement studies revealed the presence of a number of microorganisms: Escherichia coli, Escherichia coli haemolitica, Proteus vulgaris, Staphylococcus epidermidis, Enterobacter cloacae and Enterobacter faecalis in different percentages. In determining the sensitivity of isolated cultures to antibacterial drugs, it has been established that Staphylococcus aureus cultures are sensitive to all antibiotics; Proteus vulgaris show resistance to ampicillin, amoxicillin; Escherichia coli haemolytica is resistant to ampicillin.
Bean, N.H., & Griffin, P.M.G. (1990). Foodborne disease outbreaks in the US 1973–1987: Pathogens, vehicles, and trends. J. Food Prot. 53(9), 804–817. doi: 10.4315/0362-028X-53.9.804.
Mead, P.S., Slutsker, L., Dietz, V., McCraig, L.F., Bresee, J.S., Shapiro, C., Griffin, P.M., & Tauxe, R.V. (1999). Food-related illness and death in the United States. Emerg. Infect. Dis., 5(5), 607–625. doi: 10.3201/eid0505.990502.
Bryan, F.L. (2001). Reflections on a career in public health: Evolving foodborne pathogens, environmental health, and food safety programs. J. Environ. Health, 65(5), 14–24. https://www.ncbi.nlm.nih.gov/pubmed/12491850.
Dorn, C.R. (1993). Review of food borne outbreak of Escherichia coli O157:H7 infection in the western United States. J. Am. Vet. Med. Assoc. 203(11), 1583–1587. https://www.ncbi.nlm.nih.gov/pubmed/8288484.
Boyce, T.G., Swerdlow, D.L., & Griffin, P.M. (1995). Escherichia coli O157:H7 and the hemolyt-icuremic syndrome. N. Engl. J. Med. 333, 364–368. doi: 10.1056/NEJM199508103330608.
Holovko, A.M. (2009). Biolohichna ta henetychna bezpeka Ukrainy Mizhvid. nauk. temat. zbirnyk. “Veterynarna medytsyna”. Kharkiv, 92, 10–13 (in Ukrainian).
Vysockij, A.Je., & Baranovskaja, Z.N. (2002). Spravochnik po bakteriologicheskim metodam izyskanij v veterinarii. Izdatel'stvo Ministerstva sel'-skogo hozjajstva Respubliki Belarus' (in Russian).
Levkivska, N.D., Kurtiak, B.M., Levkivskyi, D.M., Padovskyi, A.I., Gutyj, B.V., & Semaniuk, V.I. (2017). Laboratorna diahnostyka infektsiinykh khvorob tvaryn bakterialnoi etiolohii. Lviv: SPOLOM (in Ukrainian).
Guardabassi, L. (2008). Guide to Antimicrobial Use in Animals. Blackwell Publishing. https://onlinelibrary. wiley.com/doi/book/10.1002/9781444302639.
Kisera, Y.V., Storchak, Y.G., & Bozhyk, L.Y. (2017). Vydovyi sklad tsyrkuliuiuchoi mikroflory ta yii stiikist do antybakterialnykh preparativ v umovakh PAF “Berezhnytsia” Zhydachivskoho raionu Lvivskoi oblasti Nauk. visnyk LNUVMB im. S.Z. Hzhytskoho, 19(78), 172–176. doi: 10.15421/nvlvet7835 (in Ukrainian).
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