Comparative Haematological and Biochemical Effects of Cocktail Vaccine (DHLPPi) and Abrus precatorius Seed Aqueous Extract on Canine Parvoviral Vaccinated and Unvaccinated Nigerian Local Dogs
Because of increasing incidence of resistance to infectious microorganisms, immunotherapy has been considered as an alternative/complementary to chemotherapy. More so Abrus precatorius leaf extract has been reported to have immunomodulatory effects in animals. In view of this, comparative haematology and biochemistry of cocktail vaccine (DHLPPi) and Abrus precatorius seed extract (APSE) was studied in rats. Sixteen (16) Nigerian local dogs of both sexes weighing 7.19 ± 0.46 kg, aged 15.44 ± 0.76 weeks old were divided into four groups of 4 each. Group 1, 2, 3 and 4 was administered 1 ml of normal saline (0.9%), APSE (2.72 mg/Kg), APSE (2.72 mg/Kg)+DHLPPi (1 ml) and DHLPPi (1 ml) respectively. Fourteen days after administration of the extract and vaccine, 3 ml of blood sample was collected from each of the dogs for haematology and serum biochemistry. The findings revealed significantly (P < 0.05)decreased packed cell volume, haemoglobin, erythrocytes, neutrophils, leucocytes, platelets and increased monocytes, decreased total protein, albumin, globulin, albumin/globulin ratio, alkaline phosphatase, alanine aminotransferase and aspartate aminotransferase in the group administered extract and extract/vaccine. Hence, the extract and extract/DHLPPi vaccine can be used in prevention chronic viral infection of dogs.
Adelowotan, O., Albinu, I., Adenipekun, E., & Odugbemi, T. (2008). The in vitro antimicrobial activity of Abrus precatorius (L) fabaceae extract on some clinical pathogens. Nigerian Postgraduate Medical Journal, 15(1), 32–37. https://www.ncbi.nlm.nih.gov/pubmed/18408781.
Anam, E.M. (2001). Anti-inflammatory activity of compounds isolated from the aerial parts of Abrus precatorius (Fabaceae). Phytomedicine, 8(1), 24–27. doi: 10.1078/0944-7113-00001.
Bhutia, S.K., Mallick, S.K., & Maiti, T.K. (2009). In vitro immunostimulatory properties of Abrus lectins derived peptides in tumor bearing mice. Phytomedicine, 16(8), 776–782. doi: 10.1016/j.phymed.2009.01.006.
Cheesbrough, M. (2005). District Laboratory Practice in Tropical countries. Part 1, low price edition, Cambridge, Cambridge University Press.
Cooper, E.L. (2004). Drug Discovery, CAM and Natural Products. Evid Based Complement Alternat Med., 1(3), 215–217. doi: 10.1093/ecam/neh032.
Ganesan, S., & Bhatt, R.Y. (2008). Qualitative Nature of Some Traditional Crude Drugs Available in Commercial Markets of Mumbai, Maharashtra. India Ethnobotanical Leaflets, 12, 348–360.
Garg, S.K. (2006). Abrus precatorius. In: Garg, S.K., Ed. Veterinary Toxicology, New Delhi, CBS publishers & Distributor, Darya Ganji.
Guyton, A.C., & Hall, J.E. (2007). Textbook of Medical Physiology, 11th edition, Saunders, An imprint of Elsevier, Philadelphia, Pennsylvania. http://jpkc.hactcm.edu.cn/2012yxslx/file/Textbook%20of%20Medical%20Physiology.pdf.
Jiang, X., Kanda, T., Nakamoto, S., Saito, K., Nakamura, M., Wu, S., et al. (2015). The JAK2 inhibitor AZD1480 inhibits hepatitis A virus replication in Huh7 cells. Biochemical and Biophysical Research Communications, 458(4), 908–912. doi: 10.1016/j.bbrc.2015.02.058.
Ligha, A., Jaja, B., & Numbere, N. (2009). Protective effect of Abrus precatorius seed extract follow- ing alcohol induced renal damage. European Journal of Scientific Research, 25(3), 428–436.
Mann, A., Gbate, M., & Nda Umar, A. (2003). Medicinal and economic plants of nupeland. Jube Evans Books and publication, Bida, Nigeria.
Newman, D., & Cragg, G. (2007). Natural products as sources of new drugs over the last 25 years. Journal of Natural Products, 70(3), 461–477. doi: 10.1021/np068054v.
Omage, K., Azeke M. A., & Orhue, J.N.E. (2015). Implications of oral administration of extracts of Acalypha wilkesiana leaves on serum electrolytes, urea and creatinine in normal experimental rabbits. Biokemistri, 27(2), 56–62. https://www.ajol.info/ index.php/biokem/article/view/133061.
Ramnath, V., Kuttan, G., & Kuttan, R. (2002). Immunopotentiating activity of abrin, a lectin from A. precatorius Linn. Indian J Exp Biol., 40, 910–913. https://www.ncbi.nlm.nih.gov/pubmed/12597021.
Sacks, D.B. (2009). Carbohydrates. In: Burtis CA, Ashwood ER. (eds.). Tietz Textbook of Clinical Chemistry. 3rd ed. Philadelphia.
Saganuwan, A.S. (2009). Tropical plants with antihypertensive, antiasthmatic and antidiabetic value. Journal of Herbs Species and Medicinal Plants, 15(1), 24–48.
Saganuwan, S.A. (2010). A photo album of some medicinal plants of the Nigerian middle belt. J Herb Spice Med Plants, 16(3), 219–292.
Saganuwan, S.A. (2011). A modified arithmetical method of Reed and Muench for determination of a relatively ideal median lethal dose (LD50). Afr. J. Pharm. Pharmacol., 5(12), 1543–1546. doi: 10.5897/AJPP11.393.
Saganuwan, S.A., & Gulumbe, M.L. (2005a). In vitro antimicrobial activities testing of Abrus precatorius cold water leaf extract on Salmonella typhimurium, Escherichia coli and klebsiella pneumoniae. Science Technology Research, 4(3), 70–73.
Saganuwan, S.A., & Gulumbe, M.L. (2005b). In vitro antimicrobial activities testing of Abrus precatorius cold water leaf extract on Streptococcus pyogenes and Streptococcus pneumonia. Proceeding of 2nd Annual Conference Nigerian Society Indigene Knowledge Develop, CRUTECH, Obabra, Cross River, 93–97.
Saganuwan, S.A., & Ogalue, A.O. (2008). Delayed effects of ceftriaxone on haematological and biochemical parameters of Nigerian local turkey poults. J Res Agric 2008, 5(1), 57–61. https://pdfs.semanticscholar.org/9a4d/db49db7d6ffe439110efe4d560387f56034a.pdf.
Saganuwan, S.A., & Onyeyili, P.A. (2010). Biochemical effects of aqueous leaf extract of Abrus precatorius (Jecquirity bean) in Swiss albino mice. Herba polonica, 56(3), 63–80. https://www.cabdirect.org/ cabdirect/abstract/20113179014.
Saganuwan, S.A., & Onyeyili, P.A. (2016). The Paradox of Human Equivalent Dose Formula: A Canonical case study of Abrus precatorius aqueous leaf extract in monogastric animals. Macedonian Veterinary Review, 39 (1), 23–32. doi: 10.1515/macvetrev-2015-0061.
Saganuwan, S.A., Onyeyili, P.A., & Etuk, E.U. (2014). Immuno Modulatory Potentials and Histopathological Effects of Aqueous Extract of Abrus Precatorius Leaf in Mus Musculus. Journal of Hematology Research, 1, 54–62. doi: 10.12974/2312-5411.2014.01.02.3.
Abstract views: 6 PDF Downloads: 2