Bakterycydic activity of plant eternal oils


  • V. Kovalenko State Scientific Control Institute Biotechnology and Strains of Microorganisms, Kyiv, Ukraine https://orcid.org/0000-0002-9244-6653
  • V. Garkavenko State Scientific and Research Institute of Laboratory Diagnostics and Veterinary and Sanitary Expertise, Kyiv, Ukraine https://orcid.org/0000-0003-4792-860X
  • O. Vishchur Institute of animal biology NAAS, Lviv, Ukraine https://orcid.org/0000-0003-4503-3896
  • S. Ponomaryova State Scientific-Research Control Institute of Veterinary Medicinal Products and Feed Additives, Lviv, Ukraine
  • I. Solovodzinska Lviv National Agrarian University, Dublyany, Ukraine
Keywords: vegetable oils, disinfection, test microorganisms, silver nanoparticles

Abstract

The aim of the research was to study the antibacterial effect of plant essential oils on the basis of nanoparticles of metals in vitro with the use of test microorganisms on test objects. In experiments, broths of S. aureus (strain P-209) were used. To prepare the broth culture, 25 cc of the nutrient medium was poured into the flask and 0.25 cc of the daily broth culture of microorganisms was added to it. A day later, broth culture was filtered through a sterile gauze-wool or paper filter. In a test tube with various dilutions of a disinfectant in 5 ml, 0.5 cm3 of 24-hour broth culture of the test microorganisms was applied. After 10 minutes holding the platinum loop from the flasks, samples were taken and transferred to Petri dishes from the MPA. The indicated types of work were conducted in compliance with the conditions of sterility. After 30 minutes, keeping the same interval, again took samples and carried out the next sowing on agar. After this, Petri dishes were placed in a thermostat with a temperature of 37 °С. Seeds were viewed 24 and 48 hours. A similar method was also used when conducting a study to determine the bactericidal effect of E. coli. The bactericidal activity of the drug “Barez” on the basis of nanoparticles of silver, benzalkonium chloride and essential oils in vitro with the use of test microorganisms on test objects was investigated. The drug has a high bactericidal effect on S. aureus, which exceeds the activity of phenol in 12.7 times, in comparison with E. coli – 24 times. By protein contamination of surfaces bactericide decreases in 1.9 times in relation to gram-positive microflora and in 1.4 times – in gram-negative. Complex disinfectant “Barez” starting from 0.05% concentration for 10 minutes completely inactivates E. soli and S. aureus on various materials with different structure of the surface. The investigated solutions of essential oils have a wide spectrum of antimicrobial action and can be used for disinfection in diseases of animals, the pathogens of which are equated with resistance to E. soli and S. aureus. Microorganisms, in prolonged contact with silver nanoparticles and essential oils, practically do not produce resistance to them, which is a significant advantage over antibiotics and can be used in humane and veterinary medicine.

Downloads

Download data is not yet available.

References

Bergonzelli, G.E., Donnicola, D., Porta, N., & Corthésy-Theulaz, I.E. (2003). Essential oils as components of a diet-based approach to management of Helicobacter infection. Antimicrobial Agent and Chemotherapy, 47(10), 3240–3246. doi: 10.1128/AAC.47.10.3240-3246.2003.

Borodina, A.V. (2004). Sravnitel'nyj analiz antimikrobnoj aktivnosti jefirnyh masel. Doneck: DMU, 13(1–2), 65–67 (in Russian).

Kovalenko, V.L. (2014). Metody kontroliu dezinfikui-uchykh zasobiv. Dovidnyk. K. (in Ukrainian).

Kovalenko, V.L., Napnenko, O.O., Chornyi, I., & Zagrebelnyi, A.V. (2018). The study of essential oil on antimicrobial activity and their composition towards microorganism. Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnologies. 20(87), 38–41. doi: 10.15421/nvlvet8707.

Nikolaevskij, V.V. (2000). Aromaterapija. M.:Medicina (in Russian).

Obrazhei, A.F., Kvachov, V.G., Ayshpur, O.E., & Sapeiko, V.P. (2008). Essential oils an alternative to antibiotics in respiratory infections treatment and prophylaxis in pigs. Veterynarna biotekhnolohiia. Biuleten, 12, 147–150.

Sartoratto, A., Machado, A.L.M., Delarmelina, C., Figuei-ra, G.M., Duarte, M.C.T. & Rehder, V.L.G. (2004). Composition and antimicrobial activity of essential oils from aromatic plants used in Brazil. Brazilian Journal of Microbiology, 35(4), 275–280. doi: 10.1590/S1517-83822004000300001.

Yakubchak, O.M., Khomenko, V.I., & Kovalenko, V.L. (2005). Rekomendatsii shchodo sanitarno-mikrobiolohichnoho doslidzhennia zmyviv z poverkhon test-obiektiv ta obiektiv veterynarnoho nahliadu i kontroliu. Metodychni rekomendatsii. K. (in Ukrainian).

Abstract views: 11
PDF Downloads: 4
Published
2018-12-10
How to Cite
Kovalenko, V., Garkavenko, V., Vishchur, O., Ponomaryova, S., & Solovodzinska, I. (2018). Bakterycydic activity of plant eternal oils. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 20(92), 113-116. https://doi.org/10.32718/nvlvet9223