Toxicological characteristics of the preparation FORTICEPTTM Hoof Oinment
Antibiotic resistance of the main infectious disease pathogens is one of the biggest problems of present time, which causes the need for searching for new antimicrobial medicinal substances and developing effective medicinal agents. One of the innovative medicinal preparations with the antimicrobial action, which is recommended for application for animals with hoof diseases, is ForticeptTM Hoof Oinment. Integration in the practice new medicinal preparations needs their strict toxicological control, which involves the exploring of acute and chronic toxicity and remote effects of possible side effect. The purpose of work was the determination of the acute toxicity parameters of the ForticeptTM Hoof Oinmentduring the oral administration to white laboratory mice and evaluation of the skin resorptive action of the preparation after it was administrated on rats’ skin. For determination of the acute toxicity there were used male laboratory mice with the average weight of the body 20 g – two groups with 10 animals in each. For the first group (the control one) with the help of the probe there was injected the distilled water (0.1 ml) into stomach. For the second group there was injected ForticeptTM Hoof Oinment (0.1 g), where the dose of the preparation is equal 500 mg/kg. For evaluation of the skin resorptive action of the preparation there were used 6 white rats with the average weight of the body 175 g. On the pre-prepared patch of skin there was administrated the preparation in the number that is equal 2857 mg/kg of body weight. For control there was leaved a free from preparation patch of bare skin. Exposition lasted for 4 hours. The indicators were explored in dynamics after 6, 24, 48 hours from the exposition started. After the research results there was established that ForticeptTM Hoof Oinment doesn’t cause death after its intragastric administration to the white laboratory mice in the number that is equal 5000 mg/kg of the body weight, that’s why depending on the degree of toxicity it belongs to the V toxicity class (Practically nontoxic). After one-time application of the preparation to the white rats in the number which is equal 2857 mg/kg of the body weight there wasn’t observes no death or pronounced changes in the behavior reactions, motor activity, state of the nervous system, amount of the consumed food and water. Therefore ForticeptTM Hoof Oinmentaccording to the results of the determination of the acute toxicity after its administration on the skin to the white rats depending on the degree of toxicity it belongs to the V toxicity class (Practically nontoxic). ForticeptTM Hoof Oinment doesn’t detect skin resorptive action, that points on the absence of toxic effects of the preparation due to its application on the skin.
World Health Organization (2012). The evolving threat of antimicrobial resistance : options for action. – Geneva: World Health Organization. http://www.who.int/iris/ handle/10665/44812.
Danchuk, V.V., Ishchenko, L.M., & Ishchenko, V.D. (2018). Vykorystannia polimeraznoi lantsiuhovoi reaktsii dlia vyznachennia heniv ß-laktamaz. Veterynarna medytsyna. Mizhvidomchyi tematychnyi naukovyi zbirnyk. Kharkiv, 104, 47–50 (in Ukrainian).
Feshchenko, Yu.I., Humeniuk, M.I., & Denysov, O.S. (2010). Antybiotykorezystentnist mikroorhanizmiv. Stan problemy ta shliakhy yii vyrishennia. Ukrainskyi khimioterapevtychnyi zhurnal, 1–2(23), 4–10. http://www.uf.ua/ua/terapevt/antybiotykorezystentnist-mikroorganizmiv-stan-problemy-ta-shlyahy-vyrishennya (in Ukrainian).
Kotsiumbas, I.Ia. et al. (2006). Doklinichni doslidzhennia veterynarnykh likarskykh zasobiv [za red. I.Ia. Kotsiumbasa]. Lviv: Triada Plius (in Ukrainian).
Ocenka vozdejstvija vrednyh himicheskih veshhestv na kozhnye pokrovy i obosnovanie PDU zagrjaznenija kozhi (metodicheskie ukazanija). Utv. MZ SSSR 01.11.1979, №2102–79. M., 22 (in Russian).
Abstract views: 11 PDF Downloads: 5