Histochemical method of diagnosis of mixed pasteurellosis and ascaridosis bird disease
For the first time was developed an effective and specific histochemical method for the diagnosis of mixed pasteurellosis and ascaridosis diseases of birds, which can be used in conjunction with bacteriological, serological and helminthocoproscopic studies, and in cases where it is impossible to diagnose serologic methods. The proposed method of histochemical diagnostics can be used for the detection of pasterelo-ascaridium carriers during mixed pasteurellosis and ascaridosis disease of birds. In the birds of the experimental group (clinically diseased bird), the pasteurial antigen in the histological preparations of the organs tissues after the histochemical study looked like local and diffuse clusters of oval and deep form of intense purple color and local clusters of oblong-shaped larvae with the presence of a head and a tail of blue and eggs of light purple color On histological sections of tissues and organs of the control group (clinically healthy bird), such changes were absent. For investigation of the organs of the experimental group (clinically diseased poultry), we have established the following areas of accumulation of pasteurial antigen and larvae and eggs of ascarids: in the heart – observed postponement of the antigen Pasteurella multocida in the form of strips throughout the area of the histological cut along the length of the muscle fibers and the loss of their delineation and fragmentation; in the liver – observed the diffuse location of the Pasteurella multocida antigen throughout the histological section in the lumen of the blood vessels and around them, the Disse spaces, between the hepatocytes; histochemically detected reduction of AsAT – by 84.3% and bilirubin – by 74.5%; in the muscular stomach – there was a diffuse postponement of the pasteurial antigen throughout the histologic section, but the largest accumulation was observed around the fibers of smooth muscle tissue of dark purple; in the spleen – the presence of Pasteurella multocida antigen throughout the histological section around lymphoid clusters, blood vessel walls, and intensive deposition of the antigen were observed around the splenic artery in the form of a deep, intense violet color; in the duodenum – observed diffuse deposition of the Pasteurella multocida antigen across the entire histologic area around the blood vessels, lymphatic follicles, villi of the mucous membrane, between the enterocytes, the mucosal muscle folds, the intramural ganglion nodules in the form of the deep form; Intestinal wall, blood vessels, licorice glands, larvae of ascarid species of Ascaridia galli species were observed blue, around granular grains around the dead larvae; Histochemically, decreased activity of alkaline phosphatase – by 70% and mucin – by 82.4%; in the kidneys – locally observed the presence of a Pasteurella multocida pasteur antigen around the intercellular veins, a capsule of the nephron, a circular oval-shaped distal tubule, a dark violet color; Histochemically, creatinine decreased by 45.4%.
Horalskyi, L.P., Khomych, V.T., & Kononskyi, O.I. (2016). Osnovy histolohichnoi tekhniky i mor-fofunktsionalni metody doslidzhennia u normi ta pry patolohii [Fundamentals of histological technique and morphofunctional methods of research in norm and at pathology]. Zhytomyr: Polissia (in Ukrainian).
Zon, H.A., Skrypka, M.V., & Ivanovska, L.B. (2009). Patolohoanatomichnyi roztyn tvaryn [Pathoanatomi-cal animals preparation]. Donetsk: Hlazunov R.O. (in Ukrainian).
Prudnikov, V.S., Birman, B.Ya., & Gromov, I.N. (2004). Patomorfologicheskaya diagnostika infektsionnyih bolezney ptits [Pathomorphological diagnosis of in-fectious diseases of birds]. Minsk: Biznesofset, 63–120 (in Russian).
Plys, V.M. (2018). The influence of symbionts of microbiocenosis on the indicators cytohemopoez at mixed pasteurellosis and ascaridosis disease bird. Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnologies. 20(83), 25–29. doi: 10.15421/nvlvet8305.
Plys, V.M. (2017). The influence conditionally pathogenic microflora on the development of pathological processes at the mixed pasteurellosis and ascaridosis diseases of poultry. Scientific Messenger LNUVMB, 19(78), 146–149. doi: 10.15421/nvlvet7829.
Plys, V.M. (2017). Patohistolohichni zminy pry pastere-lozno-askarydioznomu mikst zakhvoriuvanni ptytsi za hostroho ta khronichnoho perebihu [Pathogistolog-ical changes in mixed pasteurellosis and ascaridosis disease of birds in acute and chronic course]. Visnyk Zhytomyrskoho nats. ahroekoloh. un-tu, 1, 1(58), 144–153. http://ir.znau.edu.ua/handle/123456789/8079 (in Ukrainian).
Pototskyi, M.K., Omelianenko, M.M., & Pototska, L.M. (2007). Morfofunktsionalni doslidzhennia v normi i pry patolohii [Morphofunctional study of normal and pathological conditions]. Metodychni vkazivky dlia studentiv ta likariv veterynarnoi medytsyny – patomorfolohiv. Vyd. tsentr NAU. Kyiv (in Ukraini-an).
Ryibakov, S.S., Egorov, A.A., & Ryabokon, A.A. (2003). Primenenie immunogistohimicheskogo metoda s tse-lyu diagnostiki gubkoobraznoy entsefalopatii krupno-go rogatogo skota [The application of immunohisto-chemical method for the diagnosis of bovine spongi-form encephalopathy]. Vet. Meditsina, 12, 498–501 (in Russian).
Shutchenko, P.O. (2007). Imunohistokhimichnyi metod diahnostyky salmonelozu kurei [Immunohistochemi-cal method of diagnosis of chicken salmonellosis]. MTNZ Veterynarna medytsyna. Kharkiv, 88, 268–272 (in Ukrainian).
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