Influence of vitamins E and C on the indices of pseudoeozinofiles fagocytosis in chickens broilers blood of cross ROSS-308


Keywords: chicken broilers, vitamins, blood, phagocytic activity

Abstract

The purpose of the research was to clarify the effect of chicken broilers feeding in the composition of mixed fodder with Vitamins E and C on the cellular link of non-specific resistance of the organism and, in particular, the parameters of pseudoiesinophils phagocytosis in blood. The research was carried out on chickens broiler – 308 grown in the farm “Fedyuk M” in the Zolochiv district of Lviv region. The keeping of the chickens was cellular with free access to feed and water. Experiments were conducted in 4 groups of broiler chickens of 100 chicks in each according to the scheme: the control group was fed with the standard feed (SC) according to the existing norms recommended for the cross ROSS-308; the first experimental group in addition to the SC received vitamin Е 1g/10 kg of mixed fodder; the second experimental group received vitamin C 2.5 g/10 kg of mixed fodder; the third experimental groups in addition to the ration received Vitamin C 2.5 g/10 kg and Vitamin E 1 g/10 of mixed fodder. For carrying out of immunological researches in chickens blood was taken in different age periods: 27-, 34- and 41-day-old age. In the whole blood, the phagocytic activity of pseudoiesinophils (FA), phagocytic index (FI) and phagocytic number (FF) were determined. For research, the daily culture of Escherichia coli (strain VKM-125) was used. The stimulatory effect of vitamins E and C in the composition of mixed fodder for chicken broiler broilers on the indices of pseudoiesinophils phagocytosis of blood was determined. As evidenced by a significantly higher phagocytic activity, phagocytic index and phagocytic count of pseudoiselinophils in blood of chickens in the experimental groups by comparison with the control group. At the same time there was a direct correlation between phagocytic activity and phagocytic index and index in blood of chickens broiler in experimental groups. At the same time, the ability of blood pseudoiesinophils to phagocytosis of microbial cells was higher in chicken broilers, which were fed with vitamin E and C.

References

Aydemir, T., Oztürk, R., Bozkaya, L.A., & Tarhan, L. (2000). Effect of antiohidant vitamins A, C, E and trace elements Cu, Se on CuZn-SOD, GSH-Ph, CAT and LPO levels in chicken erythrocytes. Cell Biochem. Funct., 18(2), 109–115. doi: 10.1002/(SICI)1099-0844(200006)18:2<109::AID-CBF861>3.0.CO;2-2.

Chumachenko, V.J., Humachenko, V.C., Pavlenko, C.A., Humachenko, V.J., (2004). Investigation of the im-mune system, Factors influencing the resistance of an-imals. Veterinary Medicine of Ukraine, 5, 33–37.

Evstigneeva, R.P., Volkov, I.M., Chudinova, V.V., & Evstigneeva, R.P. (1998), Vitamin E as a universal an-tiohidant and stabilizer of biological membranes. Biol. Membranes, 15, 119–135.

Huberuk, V., Gutyj, B. V., & Hufriy, D. (2015). Influence of ursovit-adec and sodium selenite on the level of non- enzymic antioxidant defense of bulls organism for acute nitrate-nitrite toxicosis. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies, 17(1), 24–30. https://nvlvet.com.ua/index.php/journal/article/view/213.

Jiang, Y.H., McGeachin, R.B., & Bailey, C.A. (2000). Alpha-tocopherol, beta-carotene and retinol enrich-ment of chicken eggs. Poult. Sci., 73(7), 1137–1143. doi: 10.3382/ps.0731137.

Krasnikov, G.V. (2001). The decisive role of immunodefi-ciencies in modern poultry farming. Veterinary Medi-cine of Ukraine, 1, 14–15.

Lavryshyn, Y.Y., Varkholyak, I.S., Martyschuk, T.V., Guta, Z.А., Ivankiv, L.B., Paladischuk, О.R., Murska, S.D., Gutyj, B.V., & Gufriy, D.F. (2016). The biological significance of the antioxidant defense system of animals body. Scientific Messenger LNUVMBT named after S.Z. Gzhytskyj, 18, 2(66), 100–111. doi: 10.15421/nvlvet6622.

Martyshuk, T.V., Gutyj, B.V., & Vishchur, O.I. (2018). Indicators of functional and antioxidant liver status of rats under oxidative stress conditions and on the action of the liposomal drug “Butaselmevit”. Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnologies, 20(89), 100–107. doi: 10.32718/nvlvet8919.

Puthpongsiriporn, U., Schiedeler, S., Sell, J., & Beck, M. (2001). Effects of vitamin E and C supplementation on performance, in vitro lymphocyte proliferation, and antioxidant status of laying hens during heat stress. Poult. Sci., 80(8), 1190–2000. doi: 10.1093/ps/80.8.1190.

Ratych, I.B., Bishchur, O.I., Mudrak, D.I. (2006). Peculi-arities of the Immune Status in the Poultry and Mechanisms for its Provision. Nauk.-tehn. bullet Insti-tute of animal biology of UAAS and DNDKI vet prep. and feed. Supplements, 7, 12–16.

Rose, M.E. (1979). The immune system in birds. Journal of the Royal Society of Medicine, 72(9), 701–705. doi: 10.1177/014107687907200914.

Surai, P.F. (2000). Effect of selenium and vitamin E con-tent of the maternal diet on the antioxidant system of the yolk and the developing chick. Br Poult Sci., 41(2), 235–243. doi: 10.1080/713654909.

Vlizlo, V.V., Simonov, M.A., & Kaplinsky, V.V. (2006). Means for increasing the resistance of chickens. Veter-inary Medicine of Ukraine, 7, 42–44.
Published
2018-12-28
How to Cite
Romanovych, L., Kurtyak, B., Romanovych, M., Vishchur, O., & Mudrak, D. (2018). Influence of vitamins E and C on the indices of pseudoeozinofiles fagocytosis in chickens broilers blood of cross ROSS-308. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 20(92), 169-171. https://doi.org/10.32718/10.32718/nvlvet9235