Histological and histochemical changes in the dogs liver at poisoning isoniazid

Keywords: isoniazid, dogs, poisoning, liver, histology, histochemistry


The article presents the results of macroscopic, histological and histochemical studies of dogs liver for isoniazid poisoning. The pathoanatomical section of 18 corpses of dogs of all ages and breeds has been carried out, fragments of different parts of the liver have been selected. Liver samples were fixed in a 10% aqueous solution of neutral formalin, Carnoua and Buena liquids. The dewatering in ethanol of increasing concentration was conducted, sealed in paraffin. The sections were made on a microtome MC-2 in the thickness of 7 microns, stained with hematoxylin and eosin, according to the technique of McManus, Pearles. The produced histoplasts were examined under a light microscope Leica DM 2500 (Switzerland), photocycled by the Leica DFC450C camera using the Leica Application Suite Version 4.4 software. Macroscopically, the liver in all studied corpses of dogs is enlarged, the edges are rounded, inhomogeneously colored (from dark cherry to light brown), loose consistency, a light bloody fluid is secreted on the cut, the structure of the organ is smoothed out. Optically light in the liver of most dogs for isoniazid poisoning noted profound violations of all structures of angioarhitectonics, stroma and parenchyma of the organ. Prevalent non-fibrotic changes in the endothelium, dilatation and deformation of central veins and sinusoidal mesh with the formation of cells filled with blood plasma. The expressed dystrophy and necrobiosis of hepatocytes and Kupferov cells were combined with erythrocyte hemolysis, which was reflected by the deposition of hemosiderin in the cytoplasm of macrophages and bilirubin in hepatocytes. In rare cases, in the liver of poisoned dogs, acute congestive hyperemia, diapedeous hemorrhages in peripartal regions and non-fibrotic changes in hepatocytes were observed. On the basis of conducted morphological studies, it can be argued that derivatives of isoniazid compounds in the dog's organism have a pronounced hepatotoxic effect characterized by a severe violation of blood and lymph microcirculation and all metabolic processes in liver cells, manifested by dystrophic-non-fibrotic processes in the body.


Download data is not yet available.


Meyer, H., & Mally, J. (1912). On hydrazine derivatives of pyridine carbonic acids. Monatshefte Chemie verwandte Teile anderer Wissenschaften (German), 33, 393–414.

Kotsyumbas, H.I., Dancovych, R.S., & Vretsona, N.P. (2018). Pathomorphology and diagnosis of dogs poisoning with isoniazid. Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnologies. 20(83), 108–114. doi: 10.15421/nvlvet8321.

Schmid, D.R., Lee, J.A., Wismer, T.A., Diniz, P.P.V.P., & Murtaugh, R.J. (2017). Isoniazid toxicosis in dogs: 137 cases (2004–2014). JAVMA, 251(6), 689–695. https://www.ncbi.nlm.nih.gov/pubmed/28857697.

Hirtz, J. (1968). Les Methodes analytiques dans les recherches sur le metabolism des medicaments. Print book : French P., 153–154.

Korzhevs'kij, D.Je., & Giljarov, A.V. (2010). Osnovy gistologicheskoj tehniki. SPb.: SpecLit, 16–20 (in Russian).

Pirs, Je. (1962). Gistohimija teoreticheskaja i prikladnaja. M.: Inostr. lit. (in Russian).

Luzhnikov, E.A., & Kostomarova, L.G. (1989). Ostrye otravlenija. M.: Medicina, 155–156 (in Russian).

Schwab, C.E., & Tuschl, H. (2003). In vitro studies on the toxicity of isoniazid in different cell lines. Human and Experimental Toxicology, 22(11), 607–615. doi: 10.1191/0960327103ht401oa.

Kovalenko, V.M., Voronina, A.K., Shaiakhmetova, H.M. ta in. (2004). Vplyv eks perymentalnoi polivitaminoi kompozytsii na protsesy detoksykatsii izoniazydu v pechintsi shchuriv za umov yoho tryvaloi dii. Eksperymentalna i klinichna medytsyna, 1, 20–23 (in Ukrainian).

Abstract views: 10
PDF Downloads: 12
How to Cite
Kotsyumbas, H., & Vretsona, N. (2018). Histological and histochemical changes in the dogs liver at poisoning isoniazid. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 20(92), 197-202. https://doi.org/10.32718/nvlvet9241