DETERMINATION OF A CRITICAL ZONE OF ETHYLENE GLYCOL SOLUTION OF CRYSTAL FORMATION IN A WIDE SPECTRUM OF FREEZE-THAWING RATES


  • A. S. Salina Institute of animal science UААS of Ukraine, Kharkov
Keywords: ethylene glycol, sucrose, critical zone, solution of crystal formation, freezing rates, ultra-high rates of cooling

Abstract

The «visual» method established dependence of extent of turbidity of the environment on concentration of ethylene glycol (0¸70% [v/v]) and of freeze-thawing rates (10¸16×103 0С/min). The regression equation which simplifies determination of the minimum concentration of vitrifications solution of the cryoprotector containing ethylene glycol and providing transparency of the environment at the set heat exchange rates is received. The minimum concentration of vitrification solution of a cryoprotector for cryoconservation of mammals embryos (53 % [v/v]) consisting of ethylene glycol and sucrose (in the ratio 2:1) which provides transparency of the environment at a rate of 6´103 0С/min. is established.


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Author Biography

A. S. Salina, Institute of animal science UААS of Ukraine, Kharkov
c.b.s.

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Published
2015-03-06
How to Cite
Salina, A. S. (2015). DETERMINATION OF A CRITICAL ZONE OF ETHYLENE GLYCOL SOLUTION OF CRYSTAL FORMATION IN A WIDE SPECTRUM OF FREEZE-THAWING RATES. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 17(1), 150-156. Retrieved from https://nvlvet.com.ua/index.php/journal/article/view/285
Section
Physiological-biochemical and biotechnological ways of animal productivity increasing