DEVELOPMENT OF METHODS AND EVALUATION OF LONG–TERM STORAGE OF DOG'S BONE MARROW CELLS


  • L. A. Vodopyanova Kharkiv state zooveterinary academy, Kharkov
  • O. M. Bobrytska Kharkiv state zooveterinary academy, Kharkov
  • K. D. Yugay Kharkiv state zooveterinary academy, Kharkov
  • I. O. Zhukova Kharkiv state zooveterinary academy, Kharkov
Keywords: bone marrow cells, preservation of cells, cryopreservation, cryoprotectants, cell viability, DMSO, glycerol, polyethylenoxide

Abstract

It is known that the biological objects are stored frozen for a long time. The process of bone marrow cryopreservation without cryoprotectants  is very unfavorable for the cells, it causes the use of cryoprotectants during freezing. Action cryoprotectants reduce the negative effects of freezing and thawing, and stores the cells prior to transplantation, but incorrectly selected cryoprotectant may adversely affect the safety and the level of energy processes in the cells. The effect of cryoprotectants and freezing–thawing on the structural and morphological status of dogs bone marrow. Final concentrations of cryoprotectants used: DMSO–10%, 7%, 5%, PEO–400 – 10%, 15%, 20%, glycerol – 10%, 20%, 30%. These results characterize 7% DMSO as a cryoprotectant most effective for a lot of types of bone marrow cells of dogs, especially for cells in the early stages of differentiation. DMSO stored for more than 80% of bone marrow cells of dogs. PEO – 400 is able to maintain cells during cryopreservation, but the final stages of the process, amount of cells is greatly reduced. Glycerol in the concentrations tested, was less effective of the studied cryoprotectants.

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Author Biographies

L. A. Vodopyanova, Kharkiv state zooveterinary academy, Kharkov
PhD, associate professor, lecturer
O. M. Bobrytska, Kharkiv state zooveterinary academy, Kharkov
PhD, associate professor, lecturer
K. D. Yugay, Kharkiv state zooveterinary academy, Kharkov
PhD, associate professor, lecturer
I. O. Zhukova, Kharkiv state zooveterinary academy, Kharkov
doctor of veterinary science, Professor

References

Belous, A. M. Grischenko V. I. (1994). Kriobiologiya. – Kiev: Nauk. dumka, 430. (in Russian).

Pushkar, N. S., Tsutsaeva, A. A., Itkin, Yu. A., Shrago, M. I. (1984). Konservirovanie kostnogo mozga pri ultranizkih temperaturah s PEO–400: Metod. rekomendatsii. – M., 11. (in Russian).

Tsutsaeva, A. A., Agranenko, V. A., Fedorova, L. I. (1983). Kriokonservirovanie kletochnyih suspenziy. – Kiev: Nauk. dumka, 240. (in Russian).

Fuller, B. J. (2004). Cryoprotectants: the essential antifreezes to protect role in the frozen state / Fuller B. J. // Cryoletters. Vol. 25, № 6. – P. 375–388.

Kushida, T. (2000). A new method for bone marrow harvesting / Kushida T., Inaba M., Ikebukuro K., Ngahama T. et al. // Stem cells. – Vol. 18, № 6. – P. 453–456.

Sppurr, E. E.(2002). Cryopreserved human haematopoietic stem cells retain engraftment potential after extended (5–14 years) criostorage / Sppurr E. E., Wiggins N. E., Marsden K. A., Lowenthal R. M., Ragg S. S. // Cryobiology.– Vol. 44, №3 – P. 210–217.

Van de Ouweland, F. (1982). Enrichement and cryopreservation of bone marrow progenitor cells for autologous reinfusion / Van de Ouweland F., De Witte T., Geerdink P., Haanen C. // Cryobiology. Vol. 19, № 3. – P. 292–298.

Willhite, D. H. (1984). Dimethyl sulfoxide / Willhite D. H., Katz P. I. // J.Appl. Toxicol. – Vol. 4, №.3. – P. 155–159.

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PDF Downloads: 41
Published
2016-03-13
How to Cite
Vodopyanova, L. A., Bobrytska, O. M., Yugay, K. D., & Zhukova, I. O. (2016). DEVELOPMENT OF METHODS AND EVALUATION OF LONG–TERM STORAGE OF DOG’S BONE MARROW CELLS. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 18(1), 12-15. Retrieved from https://nvlvet.com.ua/index.php/journal/article/view/36