The determination of mineral density indices of the thigh bone by densitometry data in rats, for experimental fumonizinotoxycosis
The article deals with the analytical methods that have allowed to provide detailed information on the material content of bones and structural organizations of both inorganic and organic bone components. The information provided in the present search may increase the knowledge of the influence of fumonisins on the structure and bone in general. On the basis of osteometric measurements, the geometry of the transverse section of the middle diaphysis is determined. They included a definition both the external and internal diameters of the transverse section of the middle of the diaphysis (both in the medial-lateral, and in the anterior-posterior area). The geometric properties are calculated: cortical cross-sectional area, average relative thickness of walls and cortical index. In addition, since during the force analysis, the bone was loaded in the anterior-posterior area, the vertical cortical index, the moment of inertia of the cross-section and the radius of rotation around the medial-lateral axis were calculated. According to the results of comprehensive studies, we found that the intoxication of animals with fumonisin did not affect the body weight and the weight of the femoral bones, although in the fumonisin-induced doses of rats there was a decrease in the mechanical and geometric properties of the bones. The values of mineral density of bone tissue did not differ between groups, but in the experimental group of animals that were fumonisin intoxicated, the mineral content of bone and the percentage of bone ash were lower than in the control group. A detailed mineral analysis of bones showed that the content of Ca, Cu, Fe, Mn, Sr and Zn in bones was significantly decreased in fumonisin intoxicated rats compared to the control group of animals. In addition, changes in the structure of the bone and mineral phase (reduction in the size of bone hydroxyapatite crystals) were noted. Seventy percent of the bones consist of inorganic mineral hydroxyapatite, which includes calcium phosphate, calcium carbonate, calcium fluoride, calcium hydroxide and citrate. This inorganic component is preferably crystalline, although it may be in amorphous forms. Replacement mechanisms occurring in bone hydroxyapatite include intercrystalline exchange and recrystallization due to the dissolution and reforming of crystals with the addition of new ions to the crystalline structure, which replace the Ca 2+ or adsorbed on the surface of the crystals. The mechanisms of replacement that was marked by changes in the group of animals from intoxicated with fumonisin, changes were noted in the structure of the bone and mineral phase, there was a decrease in the size of bone hydroxyapatite crystals.
Antonissen, G., Croubels, S., Pasmans, F., Ducatelle, R., Eeckhaut, V., Devreese, M., Verlinden, M., Haesebrouck, F., Eeckhout, M., De Saeger, S., Ant-linger, B., Novak, B., Martel, A., & Van Immerseel, F. (2015). Fumonisins affect the intestinal microbial ho-meostasis in broiler chickens, predisposing to necrotic enteritis. Vet. Res., 46, 98. doi: 10.1186/s13567-015-0234-8.
EC (European Commission), (2006). Commission Rec-ommendation No 576/2006 of 17 August 2006 on the presence of deoxynivalenol, zearalenone, ochratoxin A, T-2 and HT-2 and fumonisins in products intended for animal feeding. Off. J. Eur. Union, L229: 7.
EC (European Commission), (2016). Commission Rec-ommendation No 2016/1219 of 29 July 2016 amend-ing Recommendation 2006/576/EC as regards deox-ynivalenol, zearalenone and ochratoxin A in pet food. Off. J. Eur. Union, L208: 59.FDA (Food and Drug Administration), (2001). Guidance for Industry: Fumonisin levels in human foods and animal feeds. Federal Register, 66(208), 56688–56689.
Ferretti, J.L., Cointry, G.R., Capozza, R.F., & Frost, H.M. (2003). Bone mass, bone strength, muscle-bone inter-actions, osteopenias and osteoporoses. Mech. Ageing Dev., 124(3), 269–279. https://www.ncbi.nlm.nih.gov/ pubmed/12663124.
Gelderblom, W.C.A., Snyman, S.D., Lebepe-Mazur, S., Smuts, C.M., Van Der Westhuizen, L., Marasas, W.F.O., Victor, T.C., Knasmueller, S., & Huber, W. (1996). Hepatotoxicity and carcinogenicity of the fumonisins in rats. Adv. Exp. Med. Biol., 392, 279–296. https://link.springer.com/chapter/10.1007/978-1-4899-1379-1_24.
Hodgson, S., Thomas, L., Fattore, E., Lind, P.M., Alfven, T., Hellström, L., Håkansson, H., Carubelli, G., Fanelli, R., & Jarup, L. (2008). Bone mineral density changes in relation to environmental PCB exposure. Environ. Health Persp., 116, 1162–1166. doi: 10.1289/ehp.11107.
Kotsyumbas, l., Brezvyn, O., Rudyk, G., Guta, Z., & Gutyj, B. (2016). Effect of fumonisin toxicosis on rats. Pasze przemysłowe. Lublin, 3/4, 108–115.
Ledoux, D.R., Brown, T.P., Weibking, T.S., & Rot-tinghaus, G.E. (1992). Fumonisin toxicity in broiler chicks. J. Vet. Diagn. Invest., 4, 330–333. doi: 10.1177/104063879200400317.
McKean, C., Tang, L., Tang, M., Billam, M., Wang, Z., Theodorakis, C.W., Kendall, R.J., & Wang, J.S. (2006). Comparative acute and combinative toxicity of aflatoxin B1 and fumonisin B1 in animals and hu-man cells. Food Chem. Toxicol., 44, 868–876. doi: 10.1016/j.fct.2005.11.011.
Placinta, C.M., D’Mello, J.P.F., & Macdonald, A.M.C. (1999). A review of worldwide contamination of cere-al grains and animal feed with Fusarium mycotoxins. Anim. Feed Sci. Technol., 78(1–2), 21–37. doi: 10.1016/S0377-8401(98)00278-8.
Reeves, P.G., Nielsen, F.H., & Fahey Jr., G.C. (1993). AIN-93 Purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J. Nurt., 123, 1939–1951. doi: 10.1093/jn/123.11.1939.
Riley, R.T., & Voss, K.A. (2006). Differential sensitivity of rat kidney and liver to fumonisin toxicity: organ-specific differences in toxin accumulation and sphin-goid base metabolism. Toxicol. Sci., 92(1), 335–345. doi: 10.1093/toxsci/kfj198.
Rodríguez-Estival, J., Álvarez-Lloret, P., Rodríguez-Navarro, A.B., Mateo, R. (2013). Chronic effects of lead (Pb) on bone properties in red deer and wild boar: relationship with vitamins and D3. Environ. Pollut., 174, 142–149. doi: 10.1016/j.envpol.2012.11.019.
Ross, P.F., Rice, L.G., Osweiler, G.D., & Nelson, P.E. (1992). A review and update of animal toxicoses associated with fumonisin contaminated feeds and production of fumonisins by Fusarium isolates. Mycopathologia, 117(1–2), 109–114. https://www.ncbi.nlm.nih. gov/pubmed/1513366.
Šegvić, M., & Pepeljnjak, S. (2001). Fumonisins and their effects on animal health a brief review. Vet. Arhiv., 71, 299–323.
Tomaszewska, E., Dobrowolski, P., Kostro, K., Jakubczak, A., Taszkun, I., Jaworska-Adamu, J., Żmuda, A., Rycerz, K., Muszyński, S. (2015). The ef-fect of HMB and 2-Ox administered during pregnancy on bone properties in primiparous and multiparous minks (Neivison vison). Bull.Vet. Inst. Pulawy, 59, 563–568. doi: 10.1515/bvip-2015-0084.
Tomaszewska, E., Dobrowolski, P., Muszyński, S., Kostro, K., Taszkun, I., Żmuda, A., Blicharski, T., Hułas-Stasiak, M. (2017). DON-induced changes in bone homeostasis in mink dams. J. Vet. Res., 61(3), 357–362. doi: 10.1515/jvetres-2017-0047.
Tomaszewska, E., Dobrowolski, P., Winiarska-Mieczan, A., Kwiecień, M., Tomczyk, A., Muszyński, S., & Radzki, R. (2016). Alteration in bone geometric and mechanical properties, histomorphometrical parame-ters of trabecular bone, articular cartilage, and growth plate in adolescent rats after chronic co-exposure to cadmium and lead in the case of supplementation with green, black, red and white tea. Environ. Toxicol. Pharmacol., 46, 36–44. doi: 10.1016/j.etap.2016.06.027.
Tomaszewska, E., Muszyński, S., Dobrowolski, P., Winiarska-Mieczan, A., Kwiecień, M., Tomczyk-Warunek, A., Ejtel, M., Świetlicka, I., & Gładyszewska, B. (2018). White tea is more effective in preservation of bone loss in adult rats co-exposed to lead and cadmium compared to black, red or green tea. Ann. Anim. Sci., In press. doi: 10.2478/aoas-2018-0026.
Voss, K.A., Chamberlain, W.J., Bacon, C.W., Herbert, R.A., Walters, D.D., & Norred, W.P. (1995). Species chronic feeding study of the mycotoxin FB1 in B6C3F1 mice and Fischer 344 rats. Fundam. Appl. Toxicol., 24(1), 102–110. doi: 10.1093/toxsci/24.1.102.
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