Industrial tests of different methods of soil samples testing for the presence of eggs of nematodes – pathogens of parasitic diseases of sheep

Keywords: soil research, efficiency, contamination, egg nematodes, efficiency of the method


Under current conditions, livestock farming, where a large number of animals are kept in restricted areas, creates all the prerequisites for intensive parasitic contamination of the environment. The objects of the environment, including the soil, are an important link in the development of pathogens of parasitic diseases. Out of all objects, soil plays a major role in the emergence of invasive diseases, since it is the main factor in the transmission of diseases. In this regard, the issue of studying contamination of soil with invasive elements remains relevant. Knowledge of the level of soil contamination with invasive elements enables to develop strategies for conducting economic activity taking into account its actual pollution and to plan well-grounded measures to overcome invasive diseases. However, it remains important to choose an effective research method, which today has a large number. In this regard, the purpose of our research was to find out the sensitivity of the methods of soil study on nematode eggs, in particular regarding the digestive system strontium, and the species Trichuris sp., S. papillosus, S. ovis and A. bovis. The obtained data indicate that the use of well-known (Romanenko-Hudzhabidze, Dolbin et al.) and advanced methods of sanitary-parasitological research proved to be suitable for the establishment of soil contamination by eggs of nematodes. However, the most effective method was the improved method, as it allows to detect the greatest number of nematode eggs in the samples under investigation (by 30.41 and 68.21%, as compared with the methods of Dolbin et al. and Romanenko-Gudzhabidze, respectively). In addition, the proposed method is more effective than the number of eggs per kg of soil (30.41 and 64.68%, respectively, for Dolbin et al. and Romanenko-Gudzhabidze). The high efficiency of the new method is also confirmed by the indicators of the ommogram, according to which it allows to find in the samples of soil eggs the strontium of the digestive organs, in particular Nematodirus sp., as well as nematodes of species Trichuris sp., S. papillosus, S. ovis, A. bovis. Their number was higher than Dolbin et al. and Romanenko-Gudzhabidze. Thus, the data obtained have an important theoretical and practical significance, since they allow a well-grounded choice of the method of soil study to establish its contamination with nematode eggs, taking into account the effectiveness of a particular species of the parasite.


Download data is not yet available.


Amoah, I.D., Singh, G., Stenström, T.A., & Reddy, P. (2017). Detection and quantification of soil-transmitted helminths in environmental samples: A review of current state-of-the-art and future perspectives. Acta Tropica, 169, 187–201. doi: 10.1016/j.actatropica.2017.02.014.

Barwick, R.S., Mohammed, H.O., White, M.E., & Bryant, R.B. (2000). Detection of Cryptosporidium parvum and Cryptosporidium muris in soil samples. Biology and Fertility of Soils, 31(5), 385–390. doi: 10.1007/s003749900185.

Collender, P.A., Kirby, A.E., Addiss, D.G., Freeman, M.C., & Remais, J.V. (2015). Methods for Quantification of Soil-Transmitted Helminths in Environmental Media: Current Techniques and Recent Advances. Trends in Parasitology, 31(12), 625–639. doi:

Dada, B.J.O. (1979). A new technique for the recovery of Toxocara eggs from soil. Journal of Helminthology, 53(2), 141–144. doi: 10.1017/s0022149x00005873.

Dolbin, D.A., Lutfullin, M.H., & Sokolina, F.M. (2014). Obsledovanija pochvy na jajca gel'mintov. Rossijskij parazitologicheskij zhurnal, 2, 70‒76 (in Russian).

Drab, R.R., Guschuk, I.V., Safonov, R.V., Byalkovsky, A.V., & Brezetska, O.I. (2017). Monitoring the epidemic process at geohelminthism among children in Rivne region. CHILD`S HEALTH, 12(3), 360–365. doi: 10.22141/2224-0551.12.3.2017.104227.

Dubey, J.P. (1998). Toxoplasma gondii Oocyst Survival under Defined Temperatures. The Journal of Parasitology, 84(4), 862–865. doi: 10.2307/3284606.

Dubná, S., Langrová, I., Jankovská, I., Vadlejcha, J., Pekárb, S., Nápravníka, J., & Fechtner, J. (2007). Contamination of soil with Toxocara eggs in urban (Prague) and rural areas in the Czech Republic. Veterinary Parasitology, 144(1–2), 81–86. doi: 10.1016/j.vetpar.2006.09.023.

Gaasenbeek, C. P., & Borgsteede, F.H. (1998). Studies on the survival of Ascaris suum eggs under laboratory and simulated field conditions. Veterinary Parasitology, 75(2–3), 227–234. doi: 10.1016/s0304-4017(97)00198-2.

Kotelnikov, G.A. (1984). Gelmintologicheskie issledovanija zhivotnykh i okruzhajushchej sredy. Moscow: Kolos (in Russian).

Lassen, B., Lepik, T., & Bangoura, B. (2013). Persistence of Eimeria bovis in soil. Parasitology Research, 112(7), 2481–2486. doi: 10.1007/s00436-013-3413-4.

Lélu, M., Gilot-Fromont, E., Aubert, D., Richaume, A., Afonso, E., Dupuis, E., Gotteland, C., Marnef, F., Poulle, M. L., Dumètre, A., Thulliez, P., Dardé, M.L., & Villena, I. (2011). Development of a sensitive method for Toxoplasma gondii oocyst extraction in soil. Veterinary Parasitology, 183 (1–2), 59–67. doi: 10.1016/j.vetpar.2011.06.018.

Loh, A.G., & Israf, D.A. (1998). Tests on the centrifugal flotation technique and its use in estimating the prevalence of Toxocara in soil samples from urban and suburban areas of Malaysia. Journal of Helminthology, 72(01), 39. doi: 10.1017/s0022149x0000095x.

Novozhilov, K.A., & Chernikova, E.A. (2014). Ak-tual'nost' i sovershenstvovanie sanitarno-gel'mintologicheskikh metodov issledovaniya pochvy. Meditsinskaya parazitologiya i parazitarnye bolezni, 1, 5859 (in Russian).

Novozhilov, K.A. (2014). Optimizacija metodov sanitar-no-parazitologicheskih issledovanij obektov sredy obitanija cheloveka: diss. ... kand. med. nauk: 03.02.11. Moskva, 99 (in Russian).

Oge, H., & Oge, S. (2000). Quantitative comparison of various methods for detecting eggs of Toxocara canis in samples of sand. Veterinary Parasitology, 92(1), 75–79. doi: 10.1016/s0304-4017(00)00276-4.

Roeber, F., Jex, A.R., & Gasser, R.B. (2013). Impact of gastrointestinal parasitic nematodes of sheep, and the role of advanced molecular tools for exploring epidemiology and drug resistance – An Australian perspective. Parasites & Vectors, 6(1), 153. doi: 10.1186/1756-3305-6-153.

Romero, C., Mendoza, G.D., Bustamante, L.P., Yanez, S., & Ramirez, N. (2010). Contamination and Viability of Toxocara sp. in Feces Collected from Public Parks, Streets and Dogs in Tejupilco at the Subhumid Tropic of Mexico. Journal of Animal and Veterinary Advances, 9(23), 2996–2999. doi: 10.3923/javaa.2010.2996.2999.

Sroka, J., Karamon, J., Dutkiewicz, J., Wójcik-Fatla, A., & Cencek, T. (2018). Optimization of flotation, DNA extraction and PCR methods for detection of Toxoplasma gondii oocysts in cat faeces. Annals of Agricultural and Environmental Medicine, 25(4), 680–685. doi: 10.26444/aaem/97402.

Steinbaum, L., Njenga, S.M., Kihara, J., Boehm, A.B., Davis, J., Null, C., & Pickering, A.J. (2016). Soil-Transmitted Helminth Eggs Are Present in Soil at Multiple Locations within Households in Rural Kenya. PLOS ONE, 11(6), e0157780. doi: 10.1371/journal.pone.0157780.

Stets, G., & Voloshyna, N. (2016). Toxocara canis – Bioindicator of Parasitic Soil Contamination of Technogenically Transformed Territories. The Advanced Science Journal, 2016(3), 41–44. doi: 10.15550/asj.2016.03.041.

Zilberman, A., Zimmels, Y., Starosvetsky, J., Zuckerman, U., & Armon, R. (2009). A Two-Phase Separation Method for Recovery of Cryptosporidium Oocysts from Soil Samples. Water, Air, and Soil Pollution, 203(1–4), 325–334. doi: 10.1007/s11270-009-0015-y.

Abstract views: 21
PDF Downloads: 15
How to Cite
Melnychuk, V., & Yuskiv, I. (2019). Industrial tests of different methods of soil samples testing for the presence of eggs of nematodes – pathogens of parasitic diseases of sheep. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 21(94), 9-14.